Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells

Cristóbal, José Ignacio; Duque, Javier; Usón-Casaús, Jesús M; Chacón, Rafael Barrera; Nieto, Esther López; Pérez-Merino, Eva M.

doi:10.3390/ani12202798

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…Moreover, the safe administration and the anti-inflammatory capacity of allogeneic adipose-derived mesenchymal stem cells (MSCs) in the treatment of CIE in dogs has been described in previous research (Pérez-Merino et al 2015 ; Cristóbal et al 2021 , 2022 ). Although the antioxidant properties of the MSCs, exerted either by decreasing the activity of oxidizing agents or promoting the antioxidant defenses, have been demonstrated in different diseases, such as gastrointestinal inflammation, and ischemic injuries (Eiro et al 2022 ), the variation in oxidative stress after the application of this novel cell therapy in dogs with CIE remains unexplored.…”

Section: Introductionmentioning

confidence: 89%

Oxidative stress in dogs with chronic inflammatory enteropathy treated with allogeneic mesenchymal stem cells

Cristóbal,

Duque,

Usón-Casaús

et al. 2023

Vet Res Commun

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The search for new biomarkers in patients with chronic inflammatory enteropathy (CIE) is ongoing in the human and veterinary medicine fields. Oxidative stress biomarkers (malondialdehyde [MDA], reduced glutathione [GSH], and albumin) have been studied in humans with chronic enteropathies, but among them, only albumin has been studied in dogs with CIE. Moreover, the effect of mesenchymal stem cell (MSCs) treatment with or without prednisone on these parameters has never been studied in dogs with CIE. These parameters were compared between healthy dogs (n = 12) and dogs with CIE, and before and 1, 3, 6, and 12 months after the treatment with MSCs alone (n = 9) or together with prednisone (n = 11). The relationship between the Canine Inflammatory Bowel Disease Activity Index (CIBDAI) and oxidative stress was evaluated. Albumin was the only parameter that significantly differed between dogs with CIE and healthy dogs (p = 0,037). Differences were observed only in albumin values after combined treatment with MSCs and prednisone. No differences were observed in MDA and GSH after treatment with MSCs with or without prednisone. Albumin could help stage canine CIE, as well as its prognosis, as has already been demonstrated, although it is essential to evaluate this parameter for its antioxidant capacity, and therefore it could be a good biomarker of oxidative stress in this pathology. However, the treatment with MSCs seems unable to modify any of the analyzed oxidative stress parameters.

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Section: Introductionmentioning

confidence: 89%

Oxidative stress in dogs with chronic inflammatory enteropathy treated with allogeneic mesenchymal stem cells

Cristóbal,

Duque,

Usón-Casaús

et al. 2023

Vet Res Commun

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“…12−20 Still, studies are limited and mainly focus on their use as prognostic factors or markers of disease, and the SII is mentioned in only one study. 20 To our knowledge, only one study reports the changes in the NLR and PLR associated with a surgical procedure (ovariohysterectomy) in cats, concluding that the NLR is an effective marker of stress and inflammation caused by surgery. 21 In the field of human surgery, the preoperative and postoperative determinations of these blood inflammatory markers have been employed as prognostic factors for complications, outcome and survival in cardiac, 22−24 oncologic, 25−27 digestive 28−31 and orthopaedic surgery.…”

Section: Introductionmentioning

confidence: 97%

“…In veterinary medicine, the neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and systemic immune‐inflammation index (SII) have been increasingly recognised as indicators of a systemic inflammatory response 12 . − 20 Still, studies are limited and mainly focus on their use as prognostic factors or markers of disease, and the SII is mentioned in only one study 20 . To our knowledge, only one study reports the changes in the NLR and PLR associated with a surgical procedure (ovariohysterectomy) in cats, concluding that the NLR is an effective marker of stress and inflammation caused by surgery 21 …”

Section: Introductionmentioning

confidence: 99%

Using complete blood count‐derived inflammatory markers to compare postoperative inflammation in dogs undergoing open or laparoscopic ovariectomy

et al. 2023

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Background The inflammatory response triggered in dogs after laparoscopic ovariectomy (LapOV) or ovariectomy through mini‐celiotomy (COV) has never been compared using the neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and systemic inflammatory index (SII). Methods Bitches underwent LapOV (n = 25) or COV (n = 25). NLR, PLR and SII were calculated 1 hour before ovariectomy (T0) and 2−3, 6−8 and 20−24 hours (T1−T3) after surgery. Results Surgical time was longer in the LapOV group. Changes over time were observed in the NLR, PLR and SII in both groups (p < 0.001). PLR at baseline and T1 (p = 0.03 and 0.01) and NLR, PLR and SII at T2 (p = 0.01, 0.01 and 0.009) were higher in the LapOV group than in the COV group, but they did not differ at T3. Limitations The overrepresentation of Greyhounds in the LapOV group and the short‐term follow‐up are the study's main limitations. Conclusion Although an inflammatory peak was observed 6−8 hours after COV or LapOV, it was higher after the laparoscopy. However, there was no difference in the bitches' inflammatory status 24 hours after surgery.

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“…The PLR correlates with duration of ICU stay in dogs with severe hemorrhage, and PLR and NLR correlate with illness severity 9 . In canine inflammatory bowel disease, PLR and NLR are markers of inflammation and could be useful in predicting treatment response 10‐13 . Similarly to human medicine, both white blood cell counts and ratios have predictive and prognostic value in dogs and cats with cancer 14‐20 .…”

Section: Introductionmentioning

confidence: 99%

“… 9 In canine inflammatory bowel disease, PLR and NLR are markers of inflammation and could be useful in predicting treatment response. 10 , 11 , 12 , 13 Similarly to human medicine, both white blood cell counts and ratios have predictive and prognostic value in dogs and cats with cancer. 14 , 15 , 16 , 17 , 18 , 19 , 20 The NLR is a useful tool for differentiating between dogs with meningoencephalitis of unknown origin from other forebrain diseases.…”

Section: Introductionmentioning

confidence: 99%

Retrospective evaluation of hematological ratios in canine parvovirosis: 401 cases

González‐Domínguez,

Cristobal‐Verdejo,

López‐Espinar

et al. 2023

Veterinary Internal Medicne

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BackgroundThe utility of neutrophil‐to‐lymphocyte ratio (NLR), platelet to‐lymphocyte ratio (PLR) and monocyte‐to‐lymphocyte ratio (MLR) as prognostic indicators has not been investigated in canine parvovirosis (CPV).HypothesisTo evaluate whether these hematological ratios obtained at hospital admission in CPV are associated with outcome or duration of hospitalization.Animals.Four hundred one client‐owned dogs presented with CPV. Methods‐Retrospective multicenter cohort study. Medical records were reviewed to identify dogs with CPV. Data regarding signalment, complete blood count at admission, duration of hospitalization and outcome were collected.ResultsOf the 401 dogs included in the study, 336 (83.8%) survived to discharge. The median (25th and 75th percentiles) PLR in nonsurvivors (336.56 [159.84‐635.77]) was significantly higher than in survivors (217.65 [117.67‐389.65]) (P = .003). The area under the receiver‐operating characteristic curve for nonsurvival was 0.615 (95% CI [0.593‐0.691], P = .003). A cut off of 700 showed a 21.5% sensitivity and 90% specificity for nonsurvival. No association was observed between hospitalization duration and either hematological ratios or total WBC counts. The median (25th and 75th percentiles) lymphocyte count was below reference interval in all dogs and was significantly lower in the dogs which died (0.82 × 109/L [0.5‐1.87]) than in survivors (1.27 × 109/L [0.73‐2.22]) (P = .005). The median (25th and 75th percentiles) monocyte count however was lower in survivors (0.38 × 109/L [0.29‐1.59]), than in nonsurvivors (0.73 × 109/L [0.1‐2]) (P = .002).ConclusionsEvaluation of PLR at hospital admission might be a useful marker of disease severity and could have prognostic value in dogs with CPV.

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Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells

Cited by 6 publications

References 46 publications

Oxidative stress in dogs with chronic inflammatory enteropathy treated with allogeneic mesenchymal stem cells

Oxidative stress in dogs with chronic inflammatory enteropathy treated with allogeneic mesenchymal stem cells

Using complete blood count‐derived inflammatory markers to compare postoperative inflammation in dogs undergoing open or laparoscopic ovariectomy

Retrospective evaluation of hematological ratios in canine parvovirosis: 401 cases

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