Complete Biosynthetic Pathway of the Phosphonate Phosphonothrixin: Two Distinct Thiamine Diphosphate-Dependent Enzymes Divide the Work to Form a C–C Bond
Abstract:Phosphonates often exhibit biological activities by mimicking the phosphates and carboxylates of biological molecules. The phosphonate phosphonothrixin (PTX), produced by the soil-dwelling bacterium Saccharothrix sp. ST-888, exhibits herbicidal activity. In this study, we propose a complete biosynthetic pathway for PTX by reconstituting its biosynthesis in vitro. Our intensive analysis demonstrated that two dehydrogenases together reduce phosphonopyruvate (PnPy) to 2-hydroxy-3phosphonopropanoic acid (HPPA) to … Show more
“…The biosynthetic pathway of phosphonothrixin 36 , a natural phosphonate from Saccharothrix species ST-888, which induces chlorosis of leaves, has been elucidated. 30 The pathway revealed a unique C–C bond forming reaction, performed by two thiamine diphosphate-dependent enzymes that result in acetyl group transfer.…”
A personal selection of 32 recent papers is presented covering various aspects of current developments in bioorganic chemistry and novel natural products such as chlorfortunone A from Chloranthus fortunei.
“…The biosynthetic pathway of phosphonothrixin 36 , a natural phosphonate from Saccharothrix species ST-888, which induces chlorosis of leaves, has been elucidated. 30 The pathway revealed a unique C–C bond forming reaction, performed by two thiamine diphosphate-dependent enzymes that result in acetyl group transfer.…”
A personal selection of 32 recent papers is presented covering various aspects of current developments in bioorganic chemistry and novel natural products such as chlorfortunone A from Chloranthus fortunei.
Carbon–carbon (C–C) bond formation can be accomplished by various highly chemo-, regio- and/or stereoselective enzymatic reactions, sometimes through the catalytic promiscuity of enzymes.
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