Complete and Draft Genome Sequences of Six Members of the
            <i>Aquificales</i>

Reysenbach, Anna‐Louise; Hamamura, Natsuko; Podar, Mircea; Griffiths, Emma; Ferreira, Susana; Hochstein, Rebecca; Heidelberg, John F.; Johnson, Justin; Mead, David A.; Pohorille, Andrew; Sarmiento, M Pedro José; Schweighofer, Karl J.; Seshadri, Rekha; Voytek, Mary A.

doi:10.1128/jb.01645-08

Cited by 69 publications

(74 citation statements)

References 8 publications

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“…All Campylobacter species for which genome sequence data are available, along with the related species Wolinella succinogenes (2), contain pglB orthologues, with three phylogenetically related Campylobacter species (Campylobacter concisus, Campylobacter curvus, and Campylobacter gracilis) containing two pglB orthologues. A number of environmental species from the Deltaproteobacteria and Epsilonproteobacteria, including sulfate-reducing bacteria and species isolated from deep-sea thermal vents, also contain pglB orthologues (4,27,33,36).…”

Section: Resultsmentioning

confidence: 99%

Characterization of N-Linked Protein Glycosylation in Helicobacter pullorum

et al. 2010

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The first bacterial N-linked glycosylation system was discovered in Campylobacter jejuni, and the key enzyme involved in the coupling of glycan to asparagine residues within the acceptor sequon of the glycoprotein is the oligosaccharyltransferase PglB. Emerging genome sequence data have revealed that pglB orthologues are present in a subset of species from the Deltaproteobacteria and Epsilonproteobacteria, including three Helicobacter species: H. pullorum, H. canadensis, and H. winghamensis. In contrast to C. jejuni, in which a single pglB gene is located within a larger gene cluster encoding the enzymes required for the biosynthesis of the N-linked glycan, these Helicobacter species contain two unrelated pglB genes (pglB1 and pglB2), neither of which is located within a larger locus involved in protein glycosylation. In complementation experiments, the H. pullorum PglB1 protein, but not PglB2, was able to transfer C. jejuni N-linked glycan onto an acceptor protein in Escherichia coli. Analysis of the characterized C. jejuni N-glycosylation system with an in vitro oligosaccharyltransferase assay followed by matrix-assisted laser desorption ionization (MALDI) mass spectrometry demonstrated the utility of this approach, and when applied to H. pullorum, PglB1-dependent N glycosylation with a linear pentasaccharide was observed. This reaction required an acidic residue at the ؊2 position of the N-glycosylation sequon, as for C. jejuni. Attempted insertional knockout mutagenesis of the H. pullorum pglB2 gene was unsuccessful, suggesting that it is essential. These first data on N-linked glycosylation in a second bacterial species demonstrate the similarities to, and fundamental differences from, the well-studied C. jejuni system.

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Section: Resultsmentioning

confidence: 99%

Characterization of N-Linked Protein Glycosylation in Helicobacter pullorum

et al. 2010

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“…Other bacterial groups in which all autotrophic members are believed to use the rTCA cycle are the Aquificales and the Epsilonproteobacteria. Enzymatic evidence has been obtained for several strains of Aquificales and Epsilonproteobacteria (Shiba et al 1985;Beh et al 1993;Hügler et al 2005Hügler et al , 2007Takai et al 2005), and the sequenced genomes also provide evidence for the comprehensive use of this pathway in these groups (Deckert et al 1998, Nakagawa et al 2007, Sievert et al 2008b, Reysenbach et al 2009). The operation of the rTCA cycle has also been reported for members of other bacterial groups, including the magnetotactic Alphaproteobacterium Magnetococcus sp.…”

Section: Reductive Tricarboxylic Acid Cyclementioning

confidence: 95%

Beyond the Calvin Cycle: Autotrophic Carbon Fixation in the Ocean

Hügler

Sievert

2011

Annu. Rev. Mar. Sci.

531

486

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Organisms capable of autotrophic metabolism assimilate inorganic carbon into organic carbon. They form an integral part of ecosystems by making an otherwise unavailable form of carbon available to other organisms, a central component of the global carbon cycle. For many years, the doctrine prevailed that the Calvin-Benson-Bassham (CBB) cycle is the only biochemical autotrophic CO2 fixation pathway of significance in the ocean. However, ecological, biochemical, and genomic studies carried out over the last decade have not only elucidated new pathways but also shown that autotrophic carbon fixation via pathways other than the CBB cycle can be significant. This has ramifications for our understanding of the carbon cycle and energy flow in the ocean. Here, we review the recent discoveries in the field of autotrophic carbon fixation, including the biochemistry and evolution of the different pathways, as well as their ecological relevance in various oceanic ecosystems.

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“…For most of these, only genome sequences are available. 4,5) Here we report on the thiosulfate oxidation of Hydrogenobacter thermophilus strain TK-6 and its sox genes as compared with ones described for other bacteria.…”

mentioning

confidence: 99%