Complete amino acid sequence of human placental protein 14: a progesterone-regulated uterine protein homologous to beta-lactoglobulins.

Abstract

Placental protein 14 (PP14), also known as progestagen-dependent endometrial protein and pregnancyassociated endometrial a2-globulin, is synthesized by the human secretory endometrium and decidua. We have isolated from a human decidual cDNA library clones corresponding to PP14 and deduced its entire amino acid sequence. PP14 contains 180 amino acids, 18 of which correspond to a putative signal peptide. The predicted molecular weight of the pre-PP14 is 20,555 and that of the mature protein is 18,787. PP14 is encoded by a 1-kilobase-pair mRNA that is expressed in human secretory endometrium and decidua but not in postmenopausal endometrium, placenta, liver, kidney, and adrenals. The 162-residue-long sequence of PP14 is highly homologous to .8-lactoglobulins, with a 53.4% identity with the amino acid sequence of horse fi-lactoglobulin I. The four cysteinyl residues (positions 66, 106, 119, and 160) responsible for intramolecular disulfide bridges in .-lactoglobulins are all conserved in PP14.

Binding of Zona Binding Inhibitory Factor-1 (ZIF-1) from Human Follicular Fluid on Spermatozoa

“…The HZI decreased in a dose-dependent manner as the concentration of 125 I-ZIF-1 or cold ZIF-1 increased. There is a discrepancy in the efficiency of spermatozoa zona pellucida binding inhibition of glycodelin-A between this report and two previous studies (5,11). Several possibilities might account for this.…”

The lipocalin protein family: structure and function

“…The major protein product expressed between the late luteal phase of the menstrual cycle and the first trimester of pregnancy is a progesterone-regulated lipocalin called PP14 [89,90] ; although first isolated from placenta, its principal site of synthesis is the secretory glandular epithelium of the endometrium and the decidua [91]. PP14 is also present in high concentrations in amniotic fluid, uterine luminal fluid, and at lower levels in plasma, but is not expressed by other tissues [89].…”

Gender-specific Glycosylation of Human Glycodelin Affects Its Contraceptive Activity

“…analysis of CNBr-digested glycodelin-S was performed using an on-line microbore reverse-phase high performance liquid chromatography system (Brownlee C 18 Aquapore column) coupled to a VG BioQ triple quadrupole electrospray mass spectrometer. The sample was dissolved in 0.1% trifluoroacetic acid (solvent system A) for injection on the column.…”

“…To address this anomaly, we have initiated comparative structure/function studies of GdA and GdS. GdA, whose protein sequence has been determined by cDNA sequencing (18), is a 162-amino acid glycoprotein that gives a 28-kDa band upon SDS gel electrophoresis (16). GdS shows a major band at 27 kDa and a minor band at 30 kDa when analyzed in the same manner (19).…”