Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA

Misrahi, Micheline; Atger, Michel; d'Auriol, L; Loosfelt, Hugues; Meriel, Cécile; Fridlansky, F.; Guiochon‐Mantel, Anne; Galibert, Francis; Milgröm, Edwin

doi:10.1016/0006-291x(87)91416-1

Cited by 395 publications

(127 citation statements)

References 19 publications

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“…four messengers from 1.8 to 8 kb in the chicken (Conneely et al, 1986(Conneely et al, , 1987 and six from 2.5 to 11.4 kb in man (Misrahi et al, 1987;Wei et al, 1988). The origin and functions of these multiple steroid receptor mRNAs are not yet well known.…”

Section: Discussionmentioning

confidence: 99%

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Pakdel

Féon

Gac

et al. 1991

Molecular and Cellular Endocrinology

160

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SummaryWe have previously described the cloning, sequencing and in vitro expression of a full-length rainbow trout estrogen receptor cDNA (rtER cDNA). This full cDNA randomly labelled was used to study the estrogen induction of hepatic rtER mRNA in correlation with vitellogenin (Vg) mRNA in different physiolo~cal situations.In this paper, we show that in the liver two mRNA species are under hormonal control and their level increases about g-fold after estrogen stimulation.These two mRNAs are expressed and induced in the liver as early as the hatching stage in correlation with the expression of Vg mRNA. A long-term analysis of rtER mRNA after estradiol (E2) injection shows a transient induction of the nuclear ER and its mRNA which recover to the basal level after 2 weeks. Nevertheless, a memory effect was observed on the expression of the Vg gene which does not appear to be directly related to the estrogen receptor level.

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Section: Discussionmentioning

confidence: 99%

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Pakdel

Féon

Gac

et al. 1991

Molecular and Cellular Endocrinology

160

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“…Total RNA was isolated from samples by using modified acid guanidinium-thiocyanatephenol-chloroform extraction procedures (Chomczynski & Sacchi 1987) ) and rat (M20133) ) androgen receptor, hu-man and rabbit (M12674, X03635, M11457) (Fuqua et al 1990;Greene et al 1986aGreene et al , 1986b and rat (Y00102) Koike et al 1987) estrogen receptor, human and rabbit (M15716) Misrahi et al 1987) and rat (S64044) (Park & Mayo 1991) progesterone receptor, and human and rabbit (M10277) (Nakajima-Iijima et al 1985) and rat b-actin (V01217, J00691) (Nudel et al 1983). The PCR conditions and number of cycles, which were selected after communication with Dr. Wilson (androgen receptor), computer analysis (Oligo 4.0 software, National Biosciences; and NCBI BLAST) and extensive experimentation, are shown in Table 2.…”

Section: Molecular Biological Proceduresmentioning

confidence: 99%

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Wickham

Gao

Toda

et al. 2000

Acta Ophthalmologica Scandinavica

311

213

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ABSTRACT.Purpose: Previous research has demonstrated that sex steroids exert a significant influence on the structure and function of numerous ocular tissues. To begin to explore the underlying basis of this hormone action, we examined whether various anterior and posterior tissues of the eye contain androgen, estrogen and progesterone receptor mRNAs. Methods: Tissue samples were obtained from adult male and female rats, rabbits and humans, processed for the isolation of total RNA and analyzed by RT-PCR, agarose gel electrophoresis and Southern blot hybridization. All PCR amplifications included positive and negative controls. Results: Our findings showed that androgen, estrogen and/or progesterone receptor mRNAs are present in the lacrimal gland, lacrimal gland acinar epithelial cells, meibomian gland, lid, palpebral and bulbar conjunctivae, cornea, iris/ciliary body, lens, retina/uvea, retina/choroid and retinal pigment epithelial cells of rats, rabbits or humans.Conclusions: Our findings demonstrate that sex steroid receptor mRNAs exist in a variety of ocular tissues and suggest that these sites may represent target organs for androgens, estrogens and/or progestins.

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“…This period, so-called window of implantation, lasts for 5 days starting on day 20 (day LH C7-11) of a 28-days menstrual cycle (Navot et al 1991, Wilcox et al 1999. On target cells, P 4 exerts its effects primarily through the nuclear P 4 receptor (PGR) (Tsai & O'Malley 1994), a member of the superfamily of ligand-activated transcription factors (Misrahi et al 1987). PGR is a ligand activated transcription factor (Chabbert-Buffet et al 2005) which upon P 4 binding activates its gene regulatory functions (Graham & Clarke 1997).…”

Section: Introductionmentioning

confidence: 99%

Effect of single post-ovulatory administration of mifepristone (RU486) on transcript profile during the receptive period in human endometrium

et al. 2016

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Progesterone regulates uterine function during the luteal phase and is essential for the acquisition of endometrial receptivity. The objective of the present study was to identify endometrial transcripts whose expression is altered during the window of implantation after the administration of 200 mg of the antiprogestin mifepristone, 48 h after the LH peak (LHC2, LHC0ZLH peak), and to determine the relationship of these transcripts with those regulated during the acquisition of receptivity. Endometrial samples were obtained in LHC7 from seven women of proven fertility, each one contributing with one cycle treated with placebo and another with mifepristone. Additionally, endometrial samples were obtained in LHC2 and LHC7 during a single untreated spontaneous cycle from seven normal fertile women as a reference. DNA microarrays were used to identify transcripts significantly regulated (defined as R2.0-fold change with false discovery rate below 1% using t-test) with the administration of mifepristone vs placebo, or during the transition from pre-receptive to receptive (LHC2 vs LHC7). Approximately 2000 transcripts were significantly regulated in both comparisons (mifepristone vs placebo and LHC2 vs LHC7), but only 777 of them were coincident and displayed opposite regulation except for 25. The mRNA level for eight selected genes regulated by mifepristone was confirmed by real-time RT-PCR. We conclude that not all changes in endometrial transcript levels occurring in the transition from LHC2 to LHC7 seem to be regulated by the progesterone receptor and w37% of the genes whose transcript levels changed by effect of mifepristone could be associated with the acquisition of receptivity.

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Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA

Cited by 395 publications

References 19 publications

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Effect of single post-ovulatory administration of mifepristone (RU486) on transcript profile during the receptive period in human endometrium

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