Complete Amino Acid Sequence of a Subunit from Rapeseed Protein

Bhushan, Ravi; Tyagi, Anil K.

doi:10.1007/bf03263027

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“…In addition, comprehensive reports on the isolation, composition and properties of peanut proteins have been written by Bhushan and co-workers (Bhushan and Martens, 1999;. Complete amino acid sequence of a subunit of storage protein of rapeseed has also been reported (Bhushan and Tyagi, 1998). Keeping in view the literature reports on glycinin and the work going on in this laboratory (Bhushan and Arya, 2007), experimental studies have been carried out to determine the number and kinds of subunits of glycinin and its molecular species, glycinin I and glycinin II.…”

Section: Introductionmentioning

confidence: 96%

Subunit structure of glycinin and its molecular species based on RP‐HPLC, gel electrophoresis and SEC studies

Bhushan

Arya

2008

Biomedical Chromatography

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Glycinin and its molecular species (glycinin I and glycinin II) were separated and isolated. The number and kind of subunits of glycinin, glycinin I and glycinin II were determined. Studies were carried out under different experimental conditions using slab gel electrophoresis, size-exclusion chromatography and reversed-phase high performance liquid chromatography. Gel electrophoresis was done using both continuous and discontinuous system and under varying concentrations of resolving gel. In addition, the subunits were separated by reversed phase using gradient program. Glycinin and glycinin II were found to have 12 subunits each while glycinin I showed six subunits. Molecular weight and weight ratio in each case were also determined.

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Section: Introductionmentioning

confidence: 96%