1999
DOI: 10.1006/bbrc.1998.9951
|View full text |Cite
|
Sign up to set email alerts
|

Complementation of thefol2Deletion inSaccharomyces cerevisiaeby Human andEscherichia coliGenes Encoding GTP Cyclohydrolase I

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
14
0

Year Published

2004
2004
2022
2022

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 10 publications
(14 citation statements)
references
References 16 publications
0
14
0
Order By: Relevance
“…FOL2 encodes GTP cyclohydrolase I, which catalyzes the initial step in the biosynthesis of the pteridine moiety of folate ( Fig. 1) [22,23]. In tomatoes and Arabidopsis, overexpression of the homologous gene results in a two-to four-fold increase in folate levels [24][25][26].…”
Section: Resultsmentioning
confidence: 99%
“…FOL2 encodes GTP cyclohydrolase I, which catalyzes the initial step in the biosynthesis of the pteridine moiety of folate ( Fig. 1) [22,23]. In tomatoes and Arabidopsis, overexpression of the homologous gene results in a two-to four-fold increase in folate levels [24][25][26].…”
Section: Resultsmentioning
confidence: 99%
“…Yeast strains and media: All the S. cerevisiae strains used in this study derived from the haploid strain 971/6a (MATa ade2-1 his3-11,15 leu2-3,112 ura3-1 can1 fol2D), which shows auxotrophy for folinic acid, and the isogenic strain 971/6c (FOL2) as wild-type control. Yeast cells were grown in SD medium (0.67% DIFCO yeast nitrogen base without amino acids, 2% glucose or galactose) plus appropriate supplements, as previously reported (Mancini et al 1999). When needed, folinic acid (Sigma, Italy) was added at a ¢nal concentration of 50 mg/ml.…”
Section: Methodsmentioning
confidence: 99%
“…The mutations ÁG693 (patient D35), 614T>G (patient D37) and 595C>G (patients D89/90) were introduced by PCR site-directed mutagenesis into the human GCH1 coding region, cloned under control of the yeast GAL1 promoter in plasmid pLA732, carrying the selection marker URA3 (described in Mancini et al 1999). To this purpose, the entire GCH1 coding region of plasmid pLA732 was ampli¢ed by Pfu polymerase (Stratagene, M-Medical, Italy), using the speci¢c primer (P2) carrying the desired mutation (OLP100, OLP102 and OLP114, respectively) (Table 1), in association with the OLP47 primer (below).…”
Section: Pcr Site-directed Mutagenesismentioning
confidence: 99%
See 2 more Smart Citations