Complementation and recombination between indole utilising tryptophan-3 mutants of Neurospora crassa

Ahmad, Mukhtar; Choudhury, K U; Islam, Shaikhul

doi:10.1038/hdy.1969.85

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Mutants of this type have been described in N . erassa (Ahmad, Choudhury & Islam, 1969) and Saccharomyces cereiisiae (Manney et al, 1969). It appears that, as in the above fungi, both tryptophan synthetase activites in C. lagopus are coded in the same genetic region.…”

Section: Discussionmentioning

confidence: 99%

Tryptophan Biosynthesis in Coprinus lagopus: A Genetic Analysis of Mutants

Tilby

1976

Journal of General Microbiology

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, S U M M A R YThirty-one tryptophan-requiring mutants of Coprinus lagopus have been assigned by genetic and complementation analyses to four loci designated trp-I, trp-2, trp-3 and trp-4. The trp-I and trp-3 loci were located in group 111 and trp-2 in group G of the linkage map. The trp-4 locus showed no linkage to the other trp loci or to markers in three additional linkage groups tested. From auxanographic tests and a study of accumulated biosynthetic intermediates, the enzymes controlled by each locus have been provisionally assigned. The trp-2 and trp-3 loci both appear necessary for anthranilate synthetase activity since mutants accumulated no intermediates. Only the trp-j? mutant could utilize anthranilic acid, therefore the trp-2 locus must also be involved in a subsequent step in the pathway. The trp-4 mutants utilized indole and accumulated anthranilic acid, and hence this locus is involved in the conversion of anthranilic acid to indoleglycerol phosphate. The trp-r mutants utilized only tryptophan and accumulated indoleglycerol phosphate and anthranilic acid. They are therefore blocked in the final steps of the pathway catalysed by tryptophan synthetase.

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Section: Discussionmentioning

confidence: 99%

Tryptophan Biosynthesis in Coprinus lagopus: A Genetic Analysis of Mutants

Tilby

1976

Journal of General Microbiology

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“…Complementation maps (4, 5, 9 and references therein, 582). Finestructure maps (5,540,582,1049). Reviewed as example of gene fusion (218).…”

Section: (S04)mentioning

confidence: 99%

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

259

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I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

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Rekombination

Abel

1971

Fortschritte Der Botanik

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Complementation and recombination between indole utilising tryptophan-3 mutants of Neurospora crassa

Cited by 4 publications

References 13 publications

Tryptophan Biosynthesis in Coprinus lagopus: A Genetic Analysis of Mutants

Tryptophan Biosynthesis in Coprinus lagopus: A Genetic Analysis of Mutants

Chromosomal loci of Neurospora crassa

Rekombination

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