Complementary seminovaginal microbiome in couples

Mändar, Reet; Punab, Margus; Borovkova, Natalja; Lapp, Eleri; Kiiker, Riinu; Korrovits, Paul; Metspalu, Andres; Krjutškov, Kaarel; Nõlvak, Hiie; Preem, Jens‐Konrad; Oopkaup, Kristjan; Salumets, Andres; Truu, Jaak

doi:10.1016/j.resmic.2015.03.009

Cited by 173 publications

(204 citation statements)

References 39 publications

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“…Regarding the associations between bacteria and clinical parameters (Table S5), we surprisingly showed a high number of correlations between age and bacteria – vaginal bacteria appeared to be associated with younger age, and environmental bacteria with older age, which can be explained with the different sexual activity rate. This confirms the results of our previous study showing a high concordance between semen and vaginal samples within a couple . Bacteria that were associated with lower sperm quality did not form a clear pattern; however, many Gram‐negative anaerobes were involved.…”

Section: Discussionsupporting

confidence: 90%

Seminal microbiome in men with and without prostatitis

et al. 2017

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Objectives: To profile the seminal microbiome applying next generation sequencing. Methods: Semen samples of 67 men were involved in the study (21 men with and 46 men without prostatitis). Seminal microbiomes were profiled applying the method that uses combinatorial sequence tags attached to polymerase chain reaction primers that amplify the ribosomal ribonucleic acid V6 region. Amplified polymerase chain reaction products were sequenced using an Illumina paired-end protocol on HiSeq2000 platform. Results: The most abundant phylum in semen was Firmicutes, comprising nearly half of the sequences found (median 41.7%, quartiles 28.5-47.2%) followed by Bacteroidetes, Proteobacteria and Actinobacteria. The counts of lactobacilli were higher in healthy men than prostatitis patients (27% [20.2-34.6%] vs 20.2% [4.9-25.0%]; P = 0.05), especially for Lactobacillus iners. Proteobacteria comprised higher proportions in prostatitis patients than healthy men. The species richness was higher in prostatitis patients than healthy men (inverted Simpson index 13.5 AE 5.8 vs 10.3 AE 4.0). Conclusions: The semen of chronic prostatitis patients contains fewer healthsupporting lactobacilli, and has higher species diversity than that of healthy men. Firmicutes (especially lactobacilli), Bacteroidetes, Proteobacteria and Actinobacteria comprise the highest proportion of seminal microbiome.

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Section: Discussionsupporting

confidence: 90%

Seminal microbiome in men with and without prostatitis

et al. 2017

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“…This difficulty is further compounded by the slow natural history of the disease, with time from acute HPV infection to high-grade CIN taking years to decades. In addition, numerous other confounders can impact results including smoking [46] and vaginal intercourse without the use of barrier contraception [47], which have both been associated with depletion of Lactobacillus spp. The impact of other sexual practices such as oral intercourse, use of lubricants and having multiple sexual partners is poorly understood, and the information gathered in the current observational studies is very heterogeneous as highlighted in Table 1.…”

Section: Limitations Of Current Literature and Areas For Future Studymentioning

confidence: 99%

“…Neither combined nor progesterone-only preparations were shown to be more protective than the other. Besides the use of hormonal contraceptives, other environmental factors known to influence VMB composition include smoking [46] and recent intercourse [47], both of which are associated with reduced relative abundance of L. crispatus and increase species diversity. Vaginal douching, particularly after menstruation, has been shown to significantly increase the risk of BV [48], and cessation of the practice may reduce the risk of BV [49].…”

Section: Introductionmentioning

confidence: 99%

The vaginal microbiota, human papillomavirus infection and cervical intraepithelial neoplasia: what do we know and where are we going next?

et al. 2016

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The vaginal microbiota plays a significant role in health and disease of the female reproductive tract. Next-generation sequencing techniques based upon the analysis of bacterial 16S rRNA genes permit in-depth study of vaginal microbial community structure to a level of detail not possible with standard culture-based microbiological techniques. The human papillomavirus (HPV) causes both cervical intraepithelial neoplasia (CIN) and cervical cancer. Although the virus is highly prevalent, only a small number of women have a persistent HPV infection and subsequently develop clinically significant disease. There is emerging evidence which leads us to conclude that increased diversity of vaginal microbiota combined with reduced relative abundance of Lactobacillus spp. is involved in HPV acquisition and persistence and the development of cervical precancer and cancer. In this review, we summarise the current literature and discuss potential mechanisms for the involvement of vaginal microbiota in the evolution of CIN and cervical cancer. The concept of manipulation of vaginal bacterial communities using pre- and probiotics is also discussed as an exciting prospect for the field of cervical pathology.

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“…A recent meta‐analysis and two studies (which did not exclude ‘true’ STIs or BV, and only included M. hominis culture positive samples) have suggested an association of M. hominis with infertility in men. However, M. hominis is strongly associated with several ‘true’ STIs that can cause infertility as well as with BV, which is common in women, and two recent studies indicated that sexual partners share their genital tract microbiome, suggesting that molecular detection in men is likely to reflect the carriage in their female sexual partner . BV is more common in women with infertility and is associated with tubal factor infertility as well as with poor implantation of the embryo as suggested by a study of women undergoing in vitro fertilization (IVF) .…”

Section: Menmentioning

confidence: 99%

Should we be testing for urogenital Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum in men and women? – a position statement from the European STI Guidelines Editorial Board

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et al. 2018

Acad Dermatol Venereol

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At present, we have no evidence that we are doing more good than harm detecting and subsequently treating Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum colonizations/infections. Consequently, routine testing and treatment of asymptomatic or symptomatic men and women for M. hominis, U. urealyticum and U. parvum are not recommended. Asymptomatic carriage of these bacteria is common, and the majority of individuals do not develop any disease. Although U. urealyticum has been associated with urethritis in men, it is probably not causal unless a high load is present (likely carriage in 40-80% of detected cases). The extensive testing, detection and subsequent antimicrobial treatment of these bacteria performed in some settings may result in the selection of antimicrobial resistance, in these bacteria, 'true' STI agents, as well as in the general microbiota, and substantial economic cost for society and individuals, particularly women. The commercialization of many particularly multiplex PCR assays detecting traditional non-viral STIs together with M. hominis, U. parvum and/or U. urealyticum has worsened this situation. Thus, routine screening of asymptomatic men and women or routine testing of symptomatic individuals for M. hominis, U. urealyticum and U. parvum is not recommended. If testing of men with symptomatic urethritis is undertaken, traditional STI urethritis agents such as Neisseria gonorrhoeae, Chlamydia trachomatis, M. genitalium and, in settings where relevant, Trichomonas vaginalis should be excluded prior to U. urealyticum testing and quantitative species-specific molecular diagnostic tests should be used. Only men with high U. urealyticum load should be considered for treatment; however, appropriate evidence for effective treatment regimens is lacking. In symptomatic women, bacterial vaginosis (BV) should always be tested for and treated if detected.

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Complementary seminovaginal microbiome in couples

Cited by 173 publications

References 39 publications

Seminal microbiome in men with and without prostatitis

Seminal microbiome in men with and without prostatitis

The vaginal microbiota, human papillomavirus infection and cervical intraepithelial neoplasia: what do we know and where are we going next?

Should we be testing for urogenital Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum in men and women? – a position statement from the European STI Guidelines Editorial Board

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