Complementary patterns of expression of , and in the blood-forming system of the chick embryo

ETS1 lowers capillary endothelial cell density at confluence and induces the expression of VE-cadherin

“…In situ hybridization studies of ets1 and VE-cadherin expression in the mouse embryo showed a strong correlation between the expression of both genes in the endothelial lineage (Breier et al, 1996;Grevin et al, 1993;Maroulakou et al, 1994;Vandenbunder et al, 1989). Ets1 and VEcadherin are both expressed in the yolk sac mesoderm at the time of blood islands formation around E7.5.…”

“…In vivo, Ets1 transcripts are expressed in endothelial precursors during vasculogenesis and in endothelial cells during angiogenesis in the embryo (Maroulakou et al, 1994;Pardanaud and Dieterlen-LieÁ vre, 1993;Queva et al, 1993;Vandenbunder et al, 1989). Ets1 expression is not detected in quiescent endothelial cells in adult tissues (Wernert et al, 1992), but it is induced in these cells during the formation of new blood vessels in solid tumors Wernert et al, 1992), in the granulation tissue during wound-healing (Wernert et al, 1992), and after denudation of the aortic endothelium (Tanaka et al, 1998).…”

“…Expression is also increased by angiogenic factors such as tumor necrosis factor a, ®broblast growth factor-2 ( 1 FGF-2), transforming growth factor b and vascular endothelial growth factor/vascular permeability factor (VEGF) (Iwasaka et al, 1996;Wernert et al, 1992). Ets1 expression is not restricted to the endothelium since its mRNA are also found in the somites and migrating neural crest cells in the embryo (Vandenbunder et al, 1989), and in stromal ®broblasts of invasive tumors Wernert et al, 1994). Nevertheless, the expression of Ets1 transcripts is associated with the activation of endothelial cells and the induction of angiogenesis, and these observations suggest that Ets1 plays a role in endothelial cells during the establishment of new blood vessels.…”

“…Nevertheless, the expression of Ets1 transcripts is associated with the activation of endothelial cells and the induction of angiogenesis, and these observations suggest that Ets1 plays a role in endothelial cells during the establishment of new blood vessels. Among Ets members, erg and¯i1 are coexpressed with ets1 in endothelial cells in the embryo (Vandenbunder et al, 1989;Dhordain et al, 1995;Macias et al, 1998;Mager et al, 1998), suggesting that these factors could play a redundant role with Ets1 during angiogenesis.…”

Transformation of Madin-Darby canine kidney (MDCK) epithelial cells by Epstein-Barr virus latent membrane protein 1 (LMP1) induces expression of Ets1 and invasive growth

“…Physiologically, Ets1 is expressed in various mesodermal derivatives, such as endothelial cells and mesenchymal cells (Vandenbunder et al, 1989;Queva et al, 1993;Kola et al, 1993), but is also induced in dissociating epithelial cells, during epithelial-mesenchymal transitions occurring during early development and oncogenic transformation to acquire invasive features (Gilles et al, 1996;Fafeur et al, 1997). Expression of Ets1 in tumor cells has been found to correlate with the grade of invasiveness.…”