Complementary analysis of tissue homogenates composition obtained by Vis and NIR laser excitations and Raman spectroscopy

Staniszewska-Slezak, Emilia; Malek, Kamilla; Barańska, Małgorzata

doi:10.1016/j.saa.2015.03.086

Cited by 18 publications

(6 citation statements)

References 42 publications

(146 reference statements)

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“…In particular, tumors and organs were homogenized to share the same weight‐to‐volume for a fair comparison (Figures S27 and S28, Supporting Information). [ 65 ] Blood showed the highest MBA‐peak intensity at 0.5 h post‐injection with a fast signal decrease at later time points, suggesting that RGD/DOX–pAS@AuNCs rapidly accumulated in organs/tumors and cleared from circulation. More importantly, ex vivo SERS imaging demonstrated the highest MBA‐peak intensity in tumors compared to other organs at 2 h post‐injection, and the signal in tumors remained relatively high even at 24 h post‐injection, indicating that RGD/DOX–pAS@AuNCs preferentially targeted HeLa cell‐constructed tumors (Figure 3e).…”

Section: Resultsmentioning

confidence: 99%

A Multilayered Mesoporous Gold Nanoarchitecture for Ultraeffective Near‐Infrared Light‐Controlled Chemo/Photothermal Therapy for Cancer Guided by SERS Imaging

Yin

Xia

et al. 2023

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NPs) with unique optical properties have emerged as one of the most exciting tools in nanomedicine, capable of achieving multiple tasks such as optical imaging, [2] targeted chemotherapy, [3] and localized photothermal therapy (PTT), [4][5][6] for efficient tumor theragnostics and preventing tumor recurrence. Prior to the therapy, the solid tumor can be spatially visualized by NP-based imaging techniques for precise diagnosis. Among various NPenhanced imaging technologies, surfaceenhanced Raman spectroscopy (SERS) has exhibited great potential for ultrasensitive biological imaging with advantages of low-cost, non-ionizing excitation light, the unique vibrational spectrum, ultra-narrow spectral line-width, high resistance to photo bleaching, and high signal-to-noise ratio. [7,8] Especially, it has been employed for tracking the biodistribution of injected NPs in vivo systems. For instance, Campbell et al. used Raman imaging to evaluate the biodistribution and clearance kinetics of plamonic NPs together with other techniques, including positron emission tomography (microPET), hyperspectral imaging, and inductively coupled plasma mass spectrometry (ICP-MS) over 48 h post-ingestion, [9] suggesting the reliability of translating high-sensitivity Raman contrast imaging into clinical practice. Therefore, there is high demand for developing strong NP-constructed SERS tags that show great prospects for effective optical guidance of tumor identification and imaging. Surface-enhanced Raman scattering (SERS) imaging has emerged as a promising tool for guided cancer diagnosis and synergistic therapies, such as combined chemotherapy and photothermal therapy (chemo-PTT). Yet, existing therapeutic agents often suffer from low SERS sensitivity, insufficient photothermal conversion, or/and limited drug loading capacity. Herein, a multifunctional theragnostic nanoplatform consisting of mesoporous silicacoated gold nanostar with a cyclic Arg-Gly-Asp (RGD)-coated gold nanocluster shell (named RGD-pAS@AuNC) is reported that exhibits multiple "hot spots" for pronouncedly enhanced SERS signals and improved nearinfrared (NIR)-induced photothermal conversion efficiency (85.5%), with a large capacity for high doxorubicin (DOX) loading efficiency (34.1%, named RGD/DOX-pAS@AuNC) and effective NIR-triggered DOX release. This nanoplatform shows excellent performance in xenograft tumor model of HeLa cell targeting, negligible cytotoxicity, and good stability both in vitro and in vivo. By SERS imaging, the optimal temporal distribution of injected RGD/ DOX-pAS@AuNCs at the tumor site is identified for NIR-triggered local chemo-PTT toward the tumor, achieving ultraeffective therapy in tumor cells and tumor-bearing mouse model with 5 min of NIR irradiation (0.5 W cm −2 ). This work offers a promising approach to employing SERS imaging for effective noninvasive tumor treatment by on-site triggered chemo-PTT.

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Section: Resultsmentioning

confidence: 99%

A Multilayered Mesoporous Gold Nanoarchitecture for Ultraeffective Near‐Infrared Light‐Controlled Chemo/Photothermal Therapy for Cancer Guided by SERS Imaging

Yin

Xia

et al. 2023

Small

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“…Figure 3 illustrates results of uni-and multivariate analysis (k-means cluster analysis) performed for Raman images of the cells. Raman distribution images were constructed by integration of the high-wavenumber region (2800-3030 cm −1 ) showing the presence of the stretching modes of the CH/CH2/CH3 groups of all organic components in the cells and by integration of the 780-800 cm −1 region indicative of the nucleus (breathing vibrations of thymine and cytosine with the O-P-O backbone mode) ( Figure 3A) [44]. KMC analysis was performed in order to segregate pixel Raman spectra into two main subcellular compartments, i.e., cytoplasm and nuclei ( Figure 3B).…”

Section: Electronic and Raman Features Of Isolated Eosinophils And Nementioning

confidence: 99%

Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Dorosz

Grosicki

Dybaś

et al. 2020

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Leukocytes are a part of the immune system that plays an important role in the host’s defense against viral, bacterial, and fungal infections. Among the human leukocytes, two granulocytes, neutrophils (Ne) and eosinophils (EOS) play an important role in the innate immune system. For that purpose, eosinophils and neutrophils contain specific granules containing protoporphyrin-type proteins such as eosinophil peroxidase (EPO) and myeloperoxidase (MPO), respectively, which contribute directly to their anti-infection activity. Since both proteins are structurally and functionally different, they could potentially be a marker of both cells’ types. To prove this hypothesis, UV−Vis absorption spectroscopy and Raman imaging were applied to analyze EPO and MPO and their content in leukocytes isolated from the whole blood. Moreover, leukocytes can contain lipidic structures, called lipid bodies (LBs), which are linked to the regulation of immune responses and are considered to be a marker of cell inflammation. In this work, we showed how to determine the number of LBs in two types of granulocytes, EOS and Ne, using fluorescence and coherent anti-Stokes Raman scattering (CARS) microscopy. Spectroscopic differences of EPO and MPO can be used to identify these cells in blood samples, while the detection of LBs can indicate the cell inflammation process.

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“…Additional band assignments in the fingerprint region can be found in previous studies. 31,32 In order to assess whether the suppression of the SERS signal at 642 cm −1 was due to the loss of GSH during filtration or due to a pH change, the filtrate was acidified with perchloric acid. As shown in Fig.…”

Section: Raman and Sers Spectra Of Gsh And Blood Samplesmentioning

confidence: 99%

Surface enhanced Raman spectroscopic direct determination of low molecular weight biothiols in umbilical cord whole blood

Kuligowski

El-Zahry

Sánchez-Illana

et al. 2016

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Biothiols play an essential role in a number of biological processes in living organisms including detoxification and metabolism. Fetal to neonatal transition poses a pro-oxidant threat for newborn infants, especially those born prematurely. A reliable and rapid tool for the direct determination of thiols in small volume whole blood (WB) samples would be desirable for its application in clinical practice. This study shows the feasibility of Surface Enhanced Raman Spectroscopy (SERS) using a silver colloid prepared by reduction of silver nitrate using hydroxylamine, as the SERS substrate for the quantification of thiols in WB samples after a simple precipitation step for protein removal. Bands originating from biothiols (790, 714 and 642 cm(-1)) were enhanced by the employed SERS substrate and the specificity of the detected SERS signal was tested for molecules presenting -SH functional groups. A statistically significant correlation between the obtained SERS signals and the thiol concentration measured using a chromatographic reference method in umbilical cord WB samples could be demonstrated. Using WB GSH concentrations obtained from the chromatographic reference procedure, a Partial Least Squares (PLS) regression model covering GSH concentrations from 13 to 2200 μM was calculated obtaining a root mean square error of prediction (RMSEP) of 381 μM when applied to an external test set. The developed approach uses small blood sample volumes (50 μL), which is important for clinical applications, especially in the field of neonatology. This feasibility study shows that the present approach combines all the necessary characteristics for its potential application in clinical practice.

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Complementary analysis of tissue homogenates composition obtained by Vis and NIR laser excitations and Raman spectroscopy

Cited by 18 publications

References 42 publications

A Multilayered Mesoporous Gold Nanoarchitecture for Ultraeffective Near‐Infrared Light‐Controlled Chemo/Photothermal Therapy for Cancer Guided by SERS Imaging

A Multilayered Mesoporous Gold Nanoarchitecture for Ultraeffective Near‐Infrared Light‐Controlled Chemo/Photothermal Therapy for Cancer Guided by SERS Imaging

Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Surface enhanced Raman spectroscopic direct determination of low molecular weight biothiols in umbilical cord whole blood

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