Complementarity Between the 5'- and 3'-terminal Sequences of Rice Stripe Virus RNAs

Takahashi, M.; Toriyama, Shigemitsu; Kikuchi, Yo; Hayakawa, Tetsuo; Ishihama, Akira

doi:10.1099/0022-1317-71-12-2817

Cited by 43 publications

(48 citation statements)

References 21 publications

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“…Each of the purified dsRNA1, dsRNA2, dsRNA3 or dsRNA4 was used after denaturation with methylmercuric hydroxide (Boehringer Ingelheim). cDNA synthesis was performed using the M-MLV reverse transcriptase (Gibco BRL) and the synthetic oligonucleotide primer P1 (5h ACTAG-TCGACACACAAAGTC 3h) ; the 10 nt sequence at the 3h end was obtained by two-dimensional sequence analysis (Takahashi et al, 1990). The synthesized second-strand DNA was blunt-ended with T4 DNA polymerase and ligated into SmaI-digested pUC18.…”

Section: Methodsmentioning

confidence: 99%

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The complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus Tenuivirus.

Toriyama

Kimishima

Takahashi

et al. 1998

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

“…The 5h-and 3h-terminal 10 nt of RGSV RNAs 2, 3, and 4 were determined by two-dimensional mobility shift analysis (Takahashi et al, 1990) and were the same as the terminal nucleotide sequences of other tenuiviruses. The 10 nt terminal sequence was used as a primer for cDNA synthesis or PCR amplification of genomic RNAs of RGSV.…”

Section: Terminal Sequencesmentioning

confidence: 99%

The complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus Tenuivirus.

Toriyama

Kimishima

Takahashi

et al. 1998

Journal of General Virology

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“…The 5'-and Y-terminal sequences of single-stranded vRNA from RSV are conserved among the four segments and are complementary to each other (Takahashi et al, 1990), indicating that vRNA is potentially able to form an intramolecular secondary structure in the shape of a panhandle, as found for the genomic RNA of negativestrand animal RNA viruses (Honda et al, 1990;Hsu et al, 1987). Such a unique secondary structure is believed to be important for specific recognition by vRNAdependent RNA polymerase (reviewed in Ishihama & Nagata, 1988).…”

Section: Introductionmentioning

confidence: 99%

Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies

Hamamatsu

Toriyama

Toyoda

et al. 1993

Journal of General Virology

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Rice stripe virus (RSV), the type species of the tenuivirus group, contains four RNA segments as its genome. Sequence analyses of the three smaller segments indicated that all of them have ambisense coding strategies. To examine the ambisense nature of the genomic RNAs, we synthesized in vitro the RNAs carrying the putative open reading frames (ORFs) by transcribing cDNA clones for RNA segments 2, 3 and 4 in both directions using T7 RNA polymerase and translated each RNA in vitro using two systems: reticulocyte lysates and wheatgerm extracts. We detected the proteins encoded by the ORFs present in the 5/-proximal regions of both viral RNAs (vRNAs) and their complementary RNAs (cRNAs). Translation in vitro of total vRNA generated proteins encoded by the ORFs present in the 5' regions of vRNAs. The overall results are consistent with the prediction that RSV RNAs, at least up to segment 2, are ambisense in their coding strategy.

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“…Members of both the Tenuivirus and Phlebovirus genera have a number of similarities including: multipartite genomes composed of both negative and ambisense segments; the genomic RNAs for viruses in each of these genera have eight identical nucleotides (5Ј-ACACAAAG) at the 5Ј termini of each genome segment while the 3Ј termini are conserved and complementary to the 5Ј termini; the genomes have similar arrangements of protein coding regions; and some of the encoded proteins for different viruses show significant similarity (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Transcription of the genomic RNAs yields mRNAs with capped 5Ј termini (12)(13)(14)(15)(16)(17)(18)(19)(20)(21), and between the cap and the viral RNA sequence are short stretches of nucleotides that are not of virus origin (12,14).…”

mentioning

confidence: 99%

In vivo transfer of barley stripe mosaic hordeivirus ribonucleotides to the 5′ terminus of maize stripe tenuivirus RNAs

Estabrook

Tsai

Falk

1998

Proc. Natl. Acad. Sci. U.S.A.

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The Tenuivirus maize stripe virus (MStV) shares many properties with viruses in the genus Phlebovirus of the family Bunyaviridae. Besides genome organization and gene expression strategies, one property shared by these plant-and vertebrate-infecting viruses is that transcription gives rise to virus-specific mRNAs containing nonviral 5-terminal nucleotide sequences. The 5-terminal nucleotides are believed to be derived from host mRNA sequences as a result of ''cap-snatching.'' We investigated whether specific nucleotide sequences could serve as primer donors for capsnatching in vivo. Barley (Hordeum vulgare) plants were singly and doubly infected with MStV and the Hordeivirus barley stripe mosaic virus (BSMV). A reverse transcription-PCR assay was used to identify chimeric BSMV͞MStV RNAs. Specific reverse transcription-PCR products were detected from doubly infected plants by using one PCR primer corresponding to the 5 termini of the BSMV RNAs (␣, ␤, and ␥) and a second primer complementary to MStV RNA 4. The resulting cDNAs were cloned, and nucleotide sequence analysis showed them to be chimeric, containing BSMV 5-terminal sequences as well as MStV RNA 4 sequences. All clones contained the BSMV RNA 5 primer nucleotide sequence, but they also showed characteristics common to Tenuivirus mRNAs. More than 80% of the clones contained BSMV RNA nucleotides not present on the PCR primer. Several lacked the exact 5 terminus of MStV RNA 4, a feature also seen for viruses in the Bunyaviridae. These data show that heterologous virus RNAs (BSMV) can serve as primer donors for MStV mRNA capped RNA-primed transcription in doubly infected plants.

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Complementarity Between the 5'- and 3'-terminal Sequences of Rice Stripe Virus RNAs

Cited by 43 publications

References 21 publications

The complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus Tenuivirus.

The complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus Tenuivirus.

Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies

In vivo transfer of barley stripe mosaic hordeivirus ribonucleotides to the 5′ terminus of maize stripe tenuivirus RNAs

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