Complement-mediated release of histamine from human basophils *1, *2III. Possible regulatory role of microtubules and microfilaments

Dupree, Elton; Thueson, David O.

doi:10.1016/0091-6749(77)90110-5

Cited by 23 publications

(8 citation statements)

References 21 publications

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“…The maximum BHR is attained within 5 min whereas LHR is much slower. Such an explosive release of mediators has been described in cytotoxic degranulation of human basophils [3] as well as in various experimental models in cluding passively sensitized rat peritoneal mast cells [11] and guinea pig lung [1]. The kinetics of release in blood is consistent with the sudden onset of some systemic ana phylactic reactions which may be encoun tered in clinical practice.…”

Section: Discussionsupporting

confidence: 53%

Allergen-Mediated Histamine Release from Whole Blood

Radermecker¹

1980

Int Arch Allergy Immunol

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An automated spectrofluorometric histamine assay has been applied to the study of allergen-mediated histamine release on small samples of blood. Histamine released into the plasma following incubation of blood with grass pollen, house dust or cat dander was compared in 35 nonallergic controls and 43 atopic patients sensitive to these allergens by history, skin tests and a high RAST score. 14 of the patients suffering from hay fever were examined after completion of at least 1 year of preseasonal immunotherapy. No allergen-mediated histamine release was observed in most nonallergic controls; a negligible release (< 5% of total blood histamine) was seen in about 10% of these subjects. In contrast, a significant histamine release, ranging from 6 to 48% of total blood content, was specifically induced by allergen in over 90% of patients sensitive to it. Negative results are found more frequently in atopic patients submitted to immunotherapy. In some patients, allergen-mediated histamine release on whole blood was compared to that obtained with the corresponding isolated leukocytes. As expected, a good correlation was found between both systems but anaphylactic histamine release occurs much more quickly in the blood than in the leukocyte suspension. This procedure for measuring histamine released by antigen on blood is easy to perform. Heparinized blood may be stored 48 h without modification in the anaphylactic release of histamine and eight different allergens may be tested with 10 ml of blood. It thus appears to be a useful method for exploring reaginic hypersensitivity and may function as an in vitro alternative to skin tests.

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Section: Discussionsupporting

confidence: 53%

Allergen-Mediated Histamine Release from Whole Blood

Radermecker¹

1980

Int Arch Allergy Immunol

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“…This is interesting because both morphological and pharmacological observations [6, 7, 9, 42]indicate that actin plays a role in the histamine release process in basophils and mast cells.…”

Section: Discussionmentioning

confidence: 99%

“…It has long been recognized that tubulins play a role in the histamine release process in basophils and mast cells. Tubulin-depolymerizing drugs like colchicine inhibit histamine release, while tubulin-stabilizing agents such as D 2 O enhance histamine release [6, 7, 9, 42, 43], indicating that polymerization of tubulin is a part of the process leading to histamine release. We found a similarity between the amount of histamine release and the amount of tubulin expression, thereby providing stoichiometric data suggesting that tubulins do play a role in basophil histamine release.…”

Section: Discussionmentioning

confidence: 99%

“…The tubulin-depolymerizing drugs colchicine and vinblastine inhibit histamine release from basophils [6, 9]. It has been reported that the uptake of Ca 2+ into cells upon stimulation with compound 40/80 was inhibited by colchicine and vinblastine.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, morphological data show that granules in mast cells are connected to a mesh of microfilaments that follows the granules during exocytosis [3]. The kinetics of filament actin (F-actin) polymerization and histamine release with IgE-dependent and -independent stimulants are identical [4], and incubation with the microfilament-disrupting agents cytochalasins modulates histamine release from mast cells [5, 6, 7]. Also, some results indicate that actin plays a role in the coupling between the IgE-FcεRI complex and downstream signaling pathways [8].…”

Section: Introductionmentioning

confidence: 99%

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Expression of Actin and Tubulins in Purified Human Basophil Leukocytes upon Stimulation with IL-3

Nielsen

Johnsen

Schiøtz

2004

Int Arch Allergy Immunol

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Background: Pharmacological and morphological results indicate that the cytoskeleton proteins tubulins and actin play a role in the histamine release process in basophil leukocytes and mast cells. In this report, we investigate the expression of these cytoskeleton proteins in purified human basophils upon stimulation with anti-IgE and IL-3. Methods: Human basophils were purified using a negative selection procedure. They were incubated for 6 h with anti-IgE and/or IL-3 in radiolabeling media. Proteins in the cells were analyzed by two-dimensional gel electrophoresis and autoradiography. Actin and α- and β-tubulin were identified by amino acid sequence analysis or matrix assisted laser desorption/ionisation time-of-flight mass spectrometry. The synthesis of these proteins under different experimental conditions was evaluated by densitometry. Results: Actin is regulated dose dependently by IL-3 with an optimum concentration of 100 ng/ml, corresponding to the optimal IL-3 concentration for enhancement of histamine release. The synthesis of tubulins was not significantly upregulated by IL-3 or anti-IgE alone, but there was a slight and significant upregulation of tubulins upon stimulation with both IL-3 and anti-IgE in doses optimal for maximal histamine release. Conclusions: The de novo synthesis of actin is regulated dose dependently by IL-3 in purified human basophils in short-term culture. The optimum IL-3 concentration is the same as for enhancement of histamine release, suggesting that the same processes that regulate IL-3-induced enhancement of histamine release also regulate the expression of actin. The finding that the expression of the tubulins is not upregulated by IL-3 alone suggests that regulation of tubulin synthesis is not a pathway by which IL-3 in itself primes basophil histamine release.

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Secretion

Dustin

1984

Microtubules

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Complement-mediated release of histamine from human basophils 1, 2III. Possible regulatory role of microtubules and microfilaments

Cited by 23 publications

References 21 publications

Allergen-Mediated Histamine Release from Whole Blood

Allergen-Mediated Histamine Release from Whole Blood

Expression of Actin and Tubulins in Purified Human Basophil Leukocytes upon Stimulation with IL-3

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