Complement-Fixation in Experimental and Human Poliomyelitis.

Loring, Hubert S.; Raffel, Sidney; Anderson, Jane Collier

doi:10.3181/00379727-66-16100

Cited by 15 publications

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“…Attempts to develop a complement-fixation antigen with Lansing-type virus have been reported previously by Loring, Raffel, and Anderson (15). Through the use of a concentrated, purified preparation positive results with rat, monkey, and human sera were obtained but a certain degree of non-specificity was apparent in some of their results.…”

Section: Discussionmentioning

confidence: 96%

A Specific Complement-Fixation Test for Infection With Poliomyelitis Virus

Casals

Olitsky

Anslow

1951

Journal of Experimental Medicine

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A complement-fixing antigen has been developed, using as source of material CNS tissue from newborn mice infected with the newborn mouse-adapted strain of the Lansing type, MEF1 virus. With this antigen, specific reactions have been obtained with sera from mice, cotton rats, and monkeys immunized with the Lansing-type virus, and from monkeys and chimpanzees convalescent from infection with this virus. Twenty-one of 35 human sera obtained from individuals convalescent from poliomyelitis were positive and 6 of 22 from apparently normal persons having Lansing-neutralizing antibody, while this held true for only 1 of 19 from those having no Lansing-neutralizing antibody. The fact that positive results were found in sera from patients having an infection with poliomyelitis virus of the Brunhilde type and at the same time no Lansing-neutralizing antibody brings up the possibility of the existence of a cross-reaction in complement fixation between the two types.

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Section: Discussionmentioning

confidence: 96%

A Specific Complement-Fixation Test for Infection With Poliomyelitis Virus

Casals

Olitsky

Anslow

1951

Journal of Experimental Medicine

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“…The complement fixation ( C F ) technique has been applied for the demonstration of polio antibodies by many investigators. Suspensions of infected tissues of the central nervous system from mice ( 1 , 2 ) and cotton rats (3)(4)(5)(6) ( 1 2 ) also used infected TC fluids as antigens in CF tests. The latter authors (13) studied the factors influencing the potency of polio CF antigens produced in T C systems.…”

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confidence: 99%

Studies on the Preparation of Complement Fixing Polio Antigens

Papapanagiotou

1961

Acta Pathologica Microbiologica Scandinavica

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“…The assay for Ac-globulin is carried out according to the method of Ware and Seegers ( 2 ) . 0.5 ml of the prepared plasma is diluted in a graduated cylinder 16 to 20 times with saline.…”

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confidence: 99%

“…0.5 ml of the prepared plasma is diluted in a graduated cylinder 16 to 20 times with saline. After 10 minutes, 0.4 ml samples of this final mixture are added at accurately timed intervals of 2 minutes to 0.1 ml samples of a 170 fiibrinogen sulution and the clotting times plotted as described by Ware and Seegers (2). In estimating the dilution required it is helpful to know the prothrombin content of the stored plasma as measured by the 2-stage method.…”

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confidence: 99%

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Human Plasma as a Source of Prothrombin in the Ac-Globulin Assay.

Olwin

1950

Experimental Biology and Medicine

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Viscosities measured a t 30" and 6 em H20 t Final concentrations of nucleate and sodium $ Refers to the viscosity of the heated solutions external pressure. chloride O..3rc and 1.0 35, respectively. after dialpis. obtained with potassium chloride and magnesium chloride. Fig. 1 descrilbes 'the effect of heating at various temperatures on the viscosity od aqueous and of salt-containing mixtures with nucleate. Final concentrati'ons of nucleate and of sodium chloride were 0.5 and 1 M, respectively. The period of heating was 20 minutes. .4t temperatures over 85", mixtures of nucleate and salt sometimes yielded a small amount of white precipitate. The structural c'haracter of the viscosity of the nucleate heated in the presence of salt is revealed in Fig. 2.The reversibility of the salt protection was demonstrated by dialysis. Aqueous and saltcontaining solutions of nucleate were heated, dialyzed, made up with water to equal concentrations of nucleate as measured spectrophotometrically in the ultraviolet, and heated again. Several such experiments revealed without exception that after loss of salt by dialysis the nucleate solution suffered a loss in viscosity on heating. A sample experiment is described in Table 11. An interesting finding is that not only does salt protect against the effect of heat, but also apparently against the equally marked effect of dialysis on reducing the viscosity of the nucleate solution.In another series of experiments, water and sodium chloride solutions (2M) were added to equal volumes of heated 1.0% nucleate solutions in water. Only a m'arked decrease in viscosity resulted after addition of the salt solution. The salt must be present while heating the nucleate solution in order to prevent the viscosity drop.Summary. The viscosity of aqueous solutions of sodium thymonzrcleate is reduced both by heating and by addition od sodium chloride and other salts. If, however, the nucleate solution is heated in the presence of a sufficiently high concentration of salt, there is no further decrease in viscosity beyond that induced by the salt. The protective effect of the salt is reversilble, for if the salt is removed ;by dialysis from the heated nucleate-salt mixture, the residual aqueous solution of nucleate again suffers a marked reduction in viscosity on heating.The approach to the problems of blood coagulation from the point of view of study-ing single factors received a major stimulus with ,the development of the 2-stage prothrombin assay by Warner, Brinkhous and Smith

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Complement-Fixation in Experimental and Human Poliomyelitis.

Cited by 15 publications

References 0 publications

A Specific Complement-Fixation Test for Infection With Poliomyelitis Virus

A Specific Complement-Fixation Test for Infection With Poliomyelitis Virus

Studies on the Preparation of Complement Fixing Polio Antigens

Human Plasma as a Source of Prothrombin in the Ac-Globulin Assay.

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