Complement factor I from flatfish half-smooth tongue ( Cynoglossus semilaevis ) exhibited anti-microbial activities

Xiang, Jinsong; Li, Xihong; Chen, Yadong; Lü, Yang; Yu, Mengjun; Chen, Xuejie; Zhang, Wenting; Zeng, Yan; Sun, Luming; Chen, Songlin; Sha, Zhenxia

doi:10.1016/j.dci.2015.06.010

Cited by 21 publications

(7 citation statements)

References 59 publications

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“…However, in previous report, there is no conclusion that C3 can directly kill bacteria. In teleost, several complement components can bind and inhibit the growth of bacteria (73,74). In our study, we found that PoC3 can have a direct bactericidal activity to V. harveyi and S. iniae.…”

Section: Discussionsupporting

confidence: 49%

Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity

Jia

2022

Front. Immunol.

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In the complement system, C3 is a central component in complement activation, immune defense and immune regulation. In all pathways of complement activation, the pivotal step is conversion of the component C3 to C3b and C3a, which is responsible to eliminate the pathogen and opsonization. In this study, we examined the immunological properties of C3 and its activated fragment C3a from Japanese flounder (Paralichthys olivaceus) (PoC3 and PoC3a), a teleost species with important economic value. PoC3 is composed of 1655 amino acid residues, contains the six domains and highly conserved GCGEQ sequence of the C3 family. We found that PoC3 expression occurred in nine different tissues and was upregulated by bacterial challenge. In serum, PoC3 was able to bind to a broad-spectrum of bacteria, and purified native PoC3 could directly kill specific pathogen. When PoC3 expression in Japanese flounder was knocked down by siRNA, serum complement activity was significantly decreased, and bacterial replication in fish tissues was significantly increased. Recombinant PoC3a (rPoC3a) exhibited apparent binding capacities to bacteria and Japanese flounder peripheral blood leukocytes (PBL) and induce chemotaxis of PBL. Japanese flounder administered rPoC3a exhibited enhanced resistance against bacterial infection. Taken together, these results indicate that PoC3 is likely a key factor of complement activation, and PoC3 and PoC3a are required for optimal defense against bacterial infection in teleost.

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Section: Discussionsupporting

confidence: 49%

Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity

Jia

2022

Front. Immunol.

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“…The proteins CFI and CD109 were both found to be more abundant in the cervical mucus of ewes in the luteal stage as opposed to during oestrus (Soleilhavoup et al, 2016). CFI has been described in humans as a component of the complement system (Xiang et al, 2015), while it has been proposed that CD109 may play a role in B cell-T cell interactions (Suciu-Foca et al, 1985). Soleilhavoup et al (2016) mentioned that secretions from the sheep uterus can enter the cervix and mix with cervical secretions.…”

Section: Studies On Endometrial Mrna Expression Of Uterocalin In Maresmentioning

confidence: 99%

A comparison of the uterine proteome of mares in oestrus and dioestrus

Maloney

Khan

Chenier

et al. 2018

Reprod Domestic Animals

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Contents Proteomic analysis of mare uterine flush fluid provides a minimally invasive technique for studying protein changes associated with the oestrous cycle. The aim of this study was to identify differentially abundant proteins in the uterine flush fluid of mares in oestrus and dioestrus. In this study, uterine flush fluid samples were collected from eight reproductively healthy mares in either oestrus (n = 5) or dioestrus (n = 3). Proteomic analysis was performed using liquid chromatography‐tandem mass spectrometry. Of 172 proteins identified, six proteins (immunoglobulin lambda‐like polypeptide 1, haemoglobin subunit alpha, alpha‐1B‐glycoprotein, serotransferrin, apolipoprotein A‐1, and haemoglobin subunit beta) were significantly more abundant in oestrus. These proteins may contribute to the endometrial defence system through roles in inflammation, immunity or antimicrobial activity. In other species, some of these proteins have been described as immunoglobulins, negative acute phase proteins or defence agents against micro‐organisms. During dioestrus, immunoglobulin alpha‐1 chain C region‐related, complement factor I, CD 109 antigen and uterocalin, were significantly more abundant. Research in other species suggests that these four proteins contribute to the immune response through proposed immunoregulatory characteristics, complement system involvement or roles in B cell–T cell interactions. In conclusion, ten differentially abundant proteins were identified in the uterine flush fluid of mares in oestrus and dioestrus. Targeted studies on these proteins could elucidate their role in uterine defence mechanisms during the oestrous cycle in the mare.

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“…In teleost fish, CFI genes from Ictalurus punctatus [ 22 ], Pelteobagrus vachellii [ 23 ], Cynoglossus semilaevis [ 24 ], Oncorhynchus mykiss [ 25 ], Paralichthys olivaceus [ 26 ] and Cyprinus carpio [ 27 ] have been identified, and structural information and the mRNA expression profiles during pathogen infection are revealed in these studies. In P. vachellii , the mRNA expression levels of CFI in the liver, spleen and head kidney are first up-regulated and then down-regulated by Aeromonas hydrophila challenge [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…CFI mRNA expressions are significantly increased in the liver of C . semilaevis after Vibrio anguillarum challenge [ 24 ]. During Edwardsiella tarda infection in P. olivaceus , CFI mRNA expressions peak significantly at 6 h post-infection in the spleen and kidney, decreasing at later infection hours [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Functional and Expressional Analyses Reveal the Distinct Role of Complement Factor I in Regulating Complement System Activation during GCRV Infection in Ctenopharyngodon idella

Liu

Xiao

et al. 2022

IJMS

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Complement factor I (CFI), a complement inhibitor, is well known for regulating the complement system activation by degrading complement component 3b (C3b) in animal serum, thus becoming involved in innate defense. Nevertheless, the functional mechanisms of CFI in the complement system and in host-pathogen interactions are far from being clarified in teleost fish. In the present study, we cloned and characterized the CFI gene, CiCFI, from grass carp (Ctenopharyngodon idella) and analyzed its function in degrading serum C3b and expression changes after grass carp reovirus (GCRV) infection. The open reading frame of CiCFI was found to be 2121 bp, encoding 706 amino acids with a molecular mass of 79.06 kDa. The pairwise alignments showed that CiCFI shared the highest identity (66.9%) with CFI from Carassius gibelio and the highest similarity (78.7%) with CFI from Danio rerio. The CiCFI protein was characterized by a conserved functional core Tryp_SPc domain with the catalytic triad and substrate binding sites. Phylogenetic analysis indicated that CiCFI and the homologs CFIs from other teleost fish formed a distinct evolutionary branch. Similar with the CFIs reported in mammals, the recombinant CiCFI protein could significantly reduce the C3b content in the serum, demonstrating the conserved function of CiCFI in the complement system in the grass carp. CiCFI mRNA and protein showed the highest expression level in the liver. After GCRV infection, the mRNA expressions of CiCFI were first down-regulated, then up-regulated, and then down-regulated to the initial level, while the protein expression levels maintained an overall downward trend to the late stage of infection in the liver of grass carps. Unexpectedly, the protein levels of CiCFI were also continuously down-regulated in the serum of grass carps during GCRV infection, while the content of serum C3b proteins first increases and then returns to the initial level, suggesting a distinct role of CiCFI in regulating complement activation and fish-virus interaction. Combining our previous results that complement factor D, a complement enhancer, shows continuously up-regulated expression levels in grass carps during GCRV infection, and this study may provide the further essential data for the full picture of complex complement regulation mechanism mediated by Df and CFI of the grass carp during pathogen infection.

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Complement factor I from flatfish half-smooth tongue ( Cynoglossus semilaevis ) exhibited anti-microbial activities

Cited by 21 publications

References 59 publications

Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity

Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity

A comparison of the uterine proteome of mares in oestrus and dioestrus

Functional and Expressional Analyses Reveal the Distinct Role of Complement Factor I in Regulating Complement System Activation during GCRV Infection in Ctenopharyngodon idella

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