Complement C3 participation in monocyte adhesion to different surfaces.

McNally, Amy K.; Anderson, James M.

doi:10.1073/pnas.91.21.10119

Cited by 153 publications

(123 citation statements)

References 37 publications

(30 reference statements)

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“…This protein is known to have a role in the adhesion of macrophages to surfaces. 10 The depletion of C3 from HS had no significant effect (Student t test, p Ͼ 0.05) here, however, on the adhesion of macrophage cells to the uncoated PET or the PC-coated samples. Again, significantly fewer macrophage cells (Student t test, p Ͻ 0.05) were found adhered to the PC-coated samples compared with the uncoated PET under both media conditions.…”

Section: Resultsmentioning

confidence: 91%

The effect of phosphorylcholine‐coated materials on the inflammatory response and fibrous capsule formation: In vitro and in vivo observations

Goreish

Lewis

Rose

et al. 2003

J Biomedical Materials Res

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Abstract:Several experiments were performed to compare the in vitro adhesion of human macrophage and granulocyte inflammatory cells to polyethylene terephthalate substrate and the same coated with a phosphorylcholine (PC)-based polymer. The inclusion of various types of serum at different stages in the assay indicated that protein adsorption and passivation of the surface may be responsible for reducing the number of inflammatory cells adhering to the uncoated polyethylene terephthalate controls. In all of the assays performed there was a statistically significant reduction (p Ͻ 0.05; analysis of variance) of the number of inflammatory cells adhering to the PC-coated samples, linked to the ability of these surfaces to resist protein adhesion. Implantations of PC-coated stainless steel and high-density polyethylene USP control samples were made in a rabbit intramuscular model. Histological examination of the number of inflammatory cells present around the implant sites 4 weeks postimplantation showed there were 40% less cells associated with the PC-coated samples compared with control, but this was not statistically significant. Fibrous capsule thickness, however, whereas marginally less at 4 weeks, had almost completely regressed for the PC-coated sample at 13 weeks postimplantation and was statistically thinner (p Ͻ 0.01; Mann-Whitney U test) than the high-density polyethylene USP control. These findings support the view that low biointeractions observed for PC-based technology in vitro can result in reduced inflammation and foreign body reaction in vivo.

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Section: Resultsmentioning

confidence: 91%

The effect of phosphorylcholine‐coated materials on the inflammatory response and fibrous capsule formation: In vitro and in vivo observations

Goreish

Lewis

Rose

et al. 2003

J Biomedical Materials Res

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“…It interacts with cellular matrix proteins, coagulant proteins, and the complement unit iC3b. Although CD11b mediates several leukocyte activities, such as phagocytosis and microbial recognition, to achieve innate defense, it is also critical for cellular adhesion and transendothelial migration (29)(30)(31). In assessment of cellular adhesion, ROS hi DCs indeed demonstrate a high capacity to rapidly adhere and spread across the polystyrene tissue culture vessel.…”

Section: Discussionmentioning

confidence: 99%

“…DC spreading, which was observed after nonspecific cellular adhesion onto the tissue culture polystyrene surface, was first evaluated to indicate the capacity of DCs in initiating CD11b-mediated innate mechanisms (29)(30)(31). As shown in Fig.…”

Section: Day 6 Ros Hi Dcs Exhibit Rapid Innate Cellular Responses To mentioning

confidence: 99%

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Sheng

Pietersz²,

Tang³

et al. 2010

The Journal of Immunology

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Reactive oxygen species (ROS) have been implicated in various physiological activities. However, their role in dendritic cell (DC) activation and generation has not been investigated. Using the bone marrow-derived GM-CSF–induced ex vivo DC model, we characterize how induction of ROS correlates with inflammatory DC functionality and expansion. We describe that the functionality of GM-CSF–induced DCs is distinct in two developmental stages. Whereas division of DC-committed hematopoietic progenitor cells (HPCs) neared completion by day 6, the level of ROS soared after day 4. Day 3 ROSlo DCs were highly responsive to TLR stimuli such as LPS and zymosan by rapid upregulation of CD80, CD86, and MHC class II, in contrast to the low response of day 6 ROShi DCs. ROShi DCs could not initiate and sustain a significant level of NF-κB phosphorylation in response to LPS and zymosan, although demonstrating hyperactivation of p38 MAPK by LPS, in a fashion disparate to ROSlo DCs. ROSlo DCs stimulated a higher level of allogeneic and OVA-specific T cell proliferative responses, although ROShi DCs were much more proficient in processing OVA. In response to pathogenic stimuli, ROShi DCs also demonstrated rapid cellular adhesion and H2O2 release, indicating their role in immediate microbial targeting. Moreover, HPC expansion and DC generation were dependent on the surge of ROS in an NADPH oxidase-independent manner. These findings point to the potential role of cellular ROS in mediating functionality and development of DCs from HPCs during inflammation.

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“…14,15,32,33 Activating monocytes ex vivo by adherence requires the presence of serum factors. 34 Therefore, we left the monocytes to adhere for 4 or 20 hours in the presence of 5% serum from the respective donor at the respective time point. Nested PCR is capable of detecting and quantifying minute amounts of mRNA.…”

Section: Discussionmentioning

confidence: 99%

Dietary ω-3, ω-6, and ω-9 Unsaturated Fatty Acids and Growth Factor and Cytokine Gene Expression in Unstimulated and Stimulated Monocytes

Baumann¹,

Hessel²,

Larass³

et al. 1999

ATVB

107

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Abstract-Dietary -3 fatty acids retard coronary atherosclerosis. Previously, we demonstrated that dietary -3 fatty acids reduce platelet-derived growth factor (PDGF)-A and PDGF-B mRNA levels in unstimulated, human mononuclear cells (MNCs). In a randomized, investigator-blinded intervention trial, we have now compared the effect of ingestion of 7 g/d -3, -6, or -9 fatty acids for 4 weeks versus no dietary intervention on PDGF-A, PDGF-B, heparin-bound epidermal growth factor (HB-EGF), monocyte chemoattractant protein-1 (MCP-1), and interleukin-10 gene expression in unstimulated MNCs and in monocytes that were adherence-activated ex vivo in a total of 28 volunteers. In unstimulated MNCs, mRNA steady-state levels of PDGF-A, PDGF-B, and MCP-1 were reduced by 25Ϯ10%, 31Ϯ13%, and 40Ϯ14%, respectively, after -3 fatty acid ingestion, as assessed by quantitative polymerase chain reaction (all PϽ0.05). In monocytes that were adherence-activated ex vivo for 4 and 20 hours, mRNA steady-state levels of PDGF-A, PDGF-B, and MCP-1 were reduced by 25Ϯ13%, 20Ϯ15%, and 30Ϯ8%, respectively (all PϽ0.05). Interleukin-10 and HB-EGF mRNA steady-state levels were not influenced by -3 fatty acid ingestion. Expression of all respective mRNAs in control volunteers or in those ingesting -6 or -9 fatty acids were not altered. We conclude that human gene expression for PDGF-A, PDGF-B, and MCP-1, factors thought relevant to atherosclerosis, is constitutive, is constant, and can be reduced only by dietary -3 fatty acids in unstimulated and adherence-activated monocytes. Key Words: mRNA Ⅲ platelet-derived growth factor Ⅲ monocyte chemoattractant protein-1 Ⅲ interleukin-10 Ⅲ heparin-bound epidermal growth factor

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Complement C3 participation in monocyte adhesion to different surfaces.

Abstract: As part of an ongoing investigation into the role of the monocyte/macrophage in biocompatibility, a major goal is to identify the adhesion mechanis that initiate and promote the observed in vivo morphologic progression of monocyte-to-macrophage-to-foreign body giant cell on bioma-

Cited by 153 publications

References 37 publications

The effect of phosphorylcholine‐coated materials on the inflammatory response and fibrous capsule formation: In vitro and in vivo observations

The effect of phosphorylcholine‐coated materials on the inflammatory response and fibrous capsule formation: In vitro and in vivo observations

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Dietary ω-3, ω-6, and ω-9 Unsaturated Fatty Acids and Growth Factor and Cytokine Gene Expression in Unstimulated and Stimulated Monocytes

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