Complement C3 gene polymorphisms are associated with lipid levels, but not the risk of coronary artery disease: a case-control study

Abstract

BackgroundCoronary artery disease (CAD) is the leading cause of mortality and morbidity worldwide. Previous studies have shown that complement component 3 (C3) is associated with atherosclerosis and cardiovascular risk factors.MethodsWe conducted this study to evaluate the associations between tagSNPs in the C3 gene locus and the CAD susceptibility and lipid levels in the Chinese population. A hospital-based case-control study, including 1017 subjects (580 CAD patients and 437 non-CAD controls), was conducted. TagSNPs in the C3 gene were searched and genotyped by using the polymerase chain reaction-ligase detection reaction method.ResultsThe C3 levels were positively associated with the low-density lipoprotein cholesterol (LDL-C) levels (r = 0.269, P = 0.001). Compared with those in controls, the serum C3 levels in CAD patients were significantly higher (Control: 0.94 + 0.14 g/l; CAD: 1.10 + 0.19 g/l, P < 0.001). No significant differences in genotype or allele frequencies were observed between CAD patients and controls. The minor T allele of rs2287848 was associated with low apolipoprotein A1 (ApoA1) levels in controls (Bonferroni corrected P, Pc = 0.032). Linkage disequilibrium and haplotype analysis established two haplotype blocks (Block1: rs344555-rs2277984, Block 2: rs2287848-rs11672613) and six haplotypes. No significant associations between haplotypes and the risk of CAD were observed (all Pc > 0.05).ConclusionsThe results revealed that C3 gene polymorphisms were associated with the lipid levels, but not CAD susceptibility in the Chinese population.

Effects of FSHR Gene Variants on Ovarian Response

“…PCR-LDR method is an efficient clinical detection tool with median multiplex and low cost. Cai et al (2019) detected eight SNPs on Complement C3 gene in 1017 subjects by using PCR-LDR method, and concluded that C3 gene polymorphisms were associated with the lipid levels, but not Coronary artery disease susceptibility in Chinese population. Multiplex LDR was also used in genotyping the polymorphisms on the genes of interferon gamma (IFNG) and IFNG receptor 1 (IFNGR1), six SNPs were detected in 565 subjects .…”

Impact of Tobacco on Proinflammatory IL1α Protein Expression in Smokers and Chewing Tobacco Users: A Novel Approach towards Protein-Ligand Incorporated Docking Analysis

“…PCR-LDR method is an efficient clinical detection tool with median multiplex and low cost. Cai et al (2019) detected eight SNPs on Complement C3 gene in 1017 subjects by using PCR-LDR method, and concluded that C3 gene polymorphisms were associated with the lipid levels, but not Coronary artery disease susceptibility in Chinese population. Multiplex LDR was also used in genotyping the polymorphisms on the genes of interferon gamma (IFNG) and IFNG receptor 1 (IFNGR1), six SNPs were detected in 565 subjects (Wu et al 2019).…”

“…The fluorescence-labeled products can be easily separated and identified on a DNA sequencer. PCR-LDR is convenient to test tens of SNPs in a single tube with relatively low cost, that's why it was widely used in detecting the polymorphism of several loci related to one or two diseases or phenotypes in hundreds of subjects (Cai et al 2019;Wu et al 2019;Zhang et al 2019). Multiplex PCR-LDR can be an appropriate option in testing a panel of SNPs related to clinical medications for CVD.…”

Complement C3 as a potential NAFLD predictor in an Egyptian cohort with diabetes and/or obesity

“…During recent years, several studies have investigated the role of complement system in metabolic disorders [8,9] and showed that serum complement C3 levels are linked to dyslipidemia [10], coronary heart disease [11], diabetes [12], and hypertension [13]. Obesity and insulin resistance, both risk factors for NAFLD, impaired numerous complement system components.…”