Complement Activation is Associated with the Presence of Specific Human Immunodeficiency Virus (HIV)‐Anti‐HIV Immune Complexes in Patients with Acquired Immunodeficiency Syndrome‐Related Complex or Lymphoadenopathy Syndrome

Carini, Claudio; Perricone, Roberto; Fratazzi, Candida; Fontana, Luigi; Carolis, Caterina De; D’Amelio, Raffaele; Sirianni, M. C.; Aiuti, F

doi:10.1111/j.1365-3083.1989.tb01220.x

Cited by 20 publications

(13 citation statements)

References 25 publications

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“…As these antibodies are able to cross-react with Clq, they are possibly responsible for the significantly low Clq concentration in HIV-1-positive sera [46]. Clq is known to be one of the factors responsible for the clearance of insoluble immune complexes [47], and its absence might contribute to their significantly high concentrations in individuals infected with HIV [48].…”

Section: Discussionmentioning

confidence: 99%

The envelope glycoprotein of HIV‐1 gp120 and human complement protein C1q bind to the same peptides derived from three different regions of gp41, the transmembrane glycoprotein of HIV‐1, and share antigenic homology

et al. 1994

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gp41, the transmembrane glycoprotein of HIV-1, has been shown to be non-covalently associated with gp120. We have shown that it also binds human C1q. To analyze the interaction site(s) of gp41 with these two molecules, we established an enzyme-linked immunosorbent assay (ELISA) system using recombinant soluble gp41 [amino acids (aa) 539-684] and peptides thereof. In the cell-external part of gp41 three sites (aa 526-538, aa 590-613 and aa 625-655) were found to bind both gp120 and C1q. That gp120 and C1q use the same sites was evidenced by the fact that these proteins competed with each other for the same sites in recombinant soluble gp41 and gp41 peptides. It could be demonstrated by ELISA, that rabbit antibodies against human C1q recognized gp120, and rabbit antibodies against gp120 cross-reacted with C1q. Rabbit anti-gp120, HIV-1-positive human sera and anti-gp120 obtained from such sera agglutinated sensitized sheep erythrocytes with human C1q (EAC1q). These data suggest that in addition to functional homology between C1q and gp120 structural homology between these two molecules exists. This molecular mimicry might become the basis for immunologically relevant autoimmune phenomena.

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Section: Discussionmentioning

confidence: 99%

The envelope glycoprotein of HIV‐1 gp120 and human complement protein C1q bind to the same peptides derived from three different regions of gp41, the transmembrane glycoprotein of HIV‐1, and share antigenic homology

et al. 1994

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“…APA was determined using rabbit erythrocytes in EGTA-VB following the method of Platts-Mills and Ishizaka [12]. A previously reported modification of this method measured the activity of 2sAPA [15,16]. hC4 was measured using agarose plates containing sensitised erythrocytes and ammonia-treated guinea-pig serum (C4 depleted) [17].…”

Section: Methodsmentioning

confidence: 99%

Impaired Human Ovarian Follicular Fluid Complement Function in Hereditary Angioedema

et al. 2000

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We sought and detected functionally active complement in human ovarian follicular fluid obtained during the peri-ovulatory period. All the functional complement activities tested, including total haemolytic complement, classical pathway activity, alternative pathway activity and C1 inhibitor function were present with values within the normal serum range. Active complement in follicular fluid is relevant for the function of the enzymatic multifactorial mechanism of ovulation. The presence in hereditary angioedema patients of both complement (C1 inhibitor deficiency and chronically consumed complement) and ovarian abnormalities (cystic ovaries), led us to study complement function in the follicular fluid of women of reproductive age affected with hereditary angioedema. In contrast to healthy women, hereditary angioedema patients showed dramatically reduced classical pathway activity and undetectable functional and antigenic C1 inhibitor. C4 was very low, while C3 and B were slightly reduced or within the normal serum range. This complement profile was also detected in patients' sera. Since hereditary angioedema patients often show cystic ovaries (polycystic or multifollicular), the presence of multifollicular ovaries in the two patients studied, along with complement dysfunction, may be relevant. These findings, as well as the normalisation of the ovaries found by us in hereditary angioedema patients and in the patients reported here who were undergoing danazol treatment, and the increase in C1 inhibitor and the improvement of clinical symptoms, suggest a further link between complement and ovarian function.

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“…HIV infection results in a strong activation, by HIV and HIV-circulating-immune-complexes [13,14], of both classical and alternative complement pathways [15,16]. In spite of this activation and of virus opsonization, with anti-HIV antibodies and complement or complement only, HIV seems to be protected from human complement lytic action [17,10].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of opsonized HIV entry in normal B lymphocytes

Legendre¹,

Gras²,

Krzysiek

et al. 1996

FEBS Letters

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Using our in vitro model of normal B cell infection that functions with low doses of HIV but requires virus opsonization by seropositive patient serum, and complement, we analyzed what receptors allowed virus entry. Here, we show that HIV infection of B cells occurs through 2 major receptors: the CD4 antigen and the CRIICR2 complex. These 2 pathways work independently since a complete inhibition of virus entry requires both CD4 and CD211CD35 blockade on CD4 d~ tonsiHar B cells whereas only the latter is critical on CD4-negative B cells.

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Complement Activation is Associated with the Presence of Specific Human Immunodeficiency Virus (HIV)‐Anti‐HIV Immune Complexes in Patients with Acquired Immunodeficiency Syndrome‐Related Complex or Lymphoadenopathy Syndrome

Cited by 20 publications

References 25 publications

The envelope glycoprotein of HIV‐1 gp120 and human complement protein C1q bind to the same peptides derived from three different regions of gp41, the transmembrane glycoprotein of HIV‐1, and share antigenic homology

The envelope glycoprotein of HIV‐1 gp120 and human complement protein C1q bind to the same peptides derived from three different regions of gp41, the transmembrane glycoprotein of HIV‐1, and share antigenic homology

Impaired Human Ovarian Follicular Fluid Complement Function in Hereditary Angioedema

Mechanisms of opsonized HIV entry in normal B lymphocytes

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