Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Hlaváček, Antonín; Farka, Zdeněk; Hübner, M.; Horňáková, Veronika; Němeček, Daniel; Nießner, Reinhard; Skládal, Petr; Knopp, Dietmar; Gorris, Hans H.

doi:10.1021/acs.analchem.6b01083

Cited by 75 publications

(35 citation statements)

References 38 publications

(60 reference statements)

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“…of the blank measurements) and p is the slope of the calibration curve. The factor k = 3 was used in compliance with the IUPAC rules (Inczedy et al, 1998;IUPAC, 2014). The time-dependent increase in the optical signal in the presence of diclofenac was fitted using the modified Gompertz model (Zwietering et al, 1990):…”

Section: Discussionmentioning

confidence: 99%

Optical and impedimetric study of genetically modified cells for diclofenac sensing

Guenther

Altenkirch²,

Rödel³

et al. 2019

J. Sens. Sens. Syst.

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Abstract. Whole-cell biosensors, based on genetically modified yeast cells, were employed to detect anthropogenic micropollutants (e.g. drugs). Specific stimuli, e.g. traces of drugs, lead to the induction of fluorescence in the respective cells. Receptors of the cells detect specific signal molecules and induce the formation of fluorescent proteins. In this work, genetically modified cells of the yeast Saccharomyces cerevisiae BY4741 were confined in a four-chamber microfluidic cell, providing an optical monitoring of the cell behaviour and their supply with the nutrients. The measurements of the time-dependent fluorescence intensity were performed with different concentrations of the drug diclofenac, and the sensitivity of yeast cells to diclofenac was demonstrated. Cell viability was monitored by simultaneous impedance recording.

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Section: Discussionmentioning

confidence: 99%

Optical and impedimetric study of genetically modified cells for diclofenac sensing

Guenther

Altenkirch²,

Rödel³

et al. 2019

J. Sens. Sens. Syst.

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“…Bioimaging probes are usually composed of organic fluorophores or fluorescent proteins, but they produce strong background fluorescence in the visible light region and have poor light stability, which greatly limits their biomedical applications [ 135 – 137 ]. Quantum dots (QDs) have high photostability, it is difficult to make extensive use of them in vivo due to their toxicity and side effects.…”

Section: Upconversion Nanomaterials For Biomedical Applicationsmentioning

confidence: 99%

Recent progress in the development of upconversion nanomaterials in bioimaging and disease treatment

et al. 2020

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Multifunctional lanthanide-based upconversion nanoparticles (UCNPs), which feature efficiently convert low-energy photons into high-energy photons, have attracted considerable attention in the domain of materials science and biomedical applications. Due to their unique photophysical properties, including light-emitting stability, excellent upconversion luminescence efficiency, low autofluorescence, and high detection sensitivity, and high penetration depth in samples, UCNPs have been widely applied in biomedical applications, such as biosensing, imaging and theranostics. In this review, we briefly introduced the major components of UCNPs and the luminescence mechanism. Then, we compared several common design synthesis strategies and presented their advantages and disadvantages. Several examples of the functionalization of UCNPs were given. Next, we detailed their biological applications in bioimaging and disease treatment, particularly drug delivery and photodynamic therapy, including antibacterial photodynamic therapy. Finally, the future practical applications in materials science and biomedical fields, as well as the remaining challenges to UCNPs application, were described. This review provides useful practical information and insights for the research on and application of UCNPs in the field of cancer.

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“…Based on the same principle, we can see the signal from FNPs that are attached to the fiber through linkage between the spike protein and antispike protein antibody tagged with FNPs conjugated with antihuman antibodies. Similar assays have been used to detect water pollutants [36].…”

Section: Bioconjugate Techniquesmentioning

confidence: 99%

A fiber optic–nanophotonic approach to the detection of antibodies and viral particles of COVID-19

Rajil

Sokolov

et al. 2020

Nanophotonics

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Dr. Deborah Birx, the White House Coronavirus Task Force coordinator, told NBC News on “Meet the Press” that “[T]he U.S. needs a ‘breakthrough’ in coronavirus testing to help screen Americans and get a more accurate picture of the virus’ spread.” We have been involved with biopathogen detection since the 2001 anthrax attacks and were the first to detect anthrax in real-time. A variation on the laser spectroscopic techniques we developed for the rapid detection of anthrax can be applied to detect the Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-CoV-2 virus). In addition to detecting a single virus, this technique allows us to read its surface protein structure. In particular, we have been conducting research based on a variety of quantum optical approaches aimed at improving our ability to detect Corona Virus Disease-2019 (COVID-19) viral infection. Indeed, the detection of a small concentration of antibodies, after an infection has passed, is a challenging problem. Likewise, the early detection of disease, even before a detectible antibody population has been established, is very important. Our team is researching both aspects of this problem. The paper is written to stimulate the interest of both physical and biological scientists in this important problem. It is thus written as a combination of tutorial (review) and future work (preview). We join Prof. Federico Capasso and Editor Dennis Couwenberg in expressing our appreciation to all those working so heroically on all aspects of the COVID-19 problem. And we thank Drs. Capasso and Couwenberg for their invitation to write this paper.

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Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Cited by 75 publications

References 38 publications

Optical and impedimetric study of genetically modified cells for diclofenac sensing

Optical and impedimetric study of genetically modified cells for diclofenac sensing

Recent progress in the development of upconversion nanomaterials in bioimaging and disease treatment

A fiber optic–nanophotonic approach to the detection of antibodies and viral particles of COVID-19

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