Competitive protein adsorption on polysaccharide and hyaluronate modified surfaces

Abstract

We measured adsorption of bovine serum albumin (BSA) and fibrinogen (Fg) onto six distinct bare and dextran- and hyaluronate-modified silicon surfaces created using two dextran grafting densities and three hyaluronic acid (HA) sodium salts derived from human umbilical cord, rooster comb and streptococcus zooepidemicus. Film thickness and surface morphology depended on HA molecular weight and concentration. BSA coverage was enhanced on surfaces upon competitive adsorption of BSA:Fg mixtures. Dextranization differentially reduced protein adsorption onto surfaces based on oxidation state. Hyaluronization was demonstrated to provide the greatest resistance to protein coverage, equivalent to that of the most resistant dextranized surface. Resistance to protein adsorption was independent of the type of hyaluronic acid utilized. With changing bulk protein concentration from 20 to 40 µg ml−1 for each species, Fg coverage on silicon increased by 4×, whereas both BSA and Fg adsorption on dextran and HA were far less dependent of protein bulk concentration.

Hyaluronan and dextran modified tubes resist cellular activation with blood contact

“…Dextrans were oxidized using the same procedure as described in our previous publications for silicon wafers [7;24;25;28–32;41;42]. The dextranized surface for this experiment was obtained by oxidizing dextran for 0.5 hr, with additional methodological detail having been given previously [25;30]. Similarly, for hyaluronic acid modification, PU and PVC tubes were first aminated using 1,6-Hexanediamine 60 wt.…”

“…Previously, our laboratories have demonstrated that silicon coated with either dextran or HA inhibits protein adsorption [28;30]. We have also shown that surfaces coated homogeneously or patterned with either dextran or HA, or coated with a binary pattern of dextran and HA, have differential effects for adhesion of human macrophages (THP-1 cell line) [41] and that cell morphology and secretory responses a substrate dependent [21].…”

Chitosan/siRNA functionalized titanium surface via a layer-by-layer approach for in vitro sustained gene silencing and osteogenic promotion

“…28 On the contrary, hyaluronate is more hydrophilic and shows resistance to protein adsorption. 29 For this reason, we adhered CS as the final layer. AFM scanning was performed to observe the surface topography and measure the roughness, which in general exhibited an up-and-down evolution as well.…”

Adsorption of alginate and albumin on aluminum coatings inhibits adhesion of Escherichia coli and enhances the anti-corrosion performances of the coatings

“…Polysaccharides and proteins are the major component of a conditioning layer. Sodium alginate widely exists in several of well-known algae and bacteria [35,36] and albumin has been chosen as typical protein for biofouling research [37]. In this paper, albumin/alginate was employed as the typical biomacromolecules to build a simplified model representing a conditioning layer.…”