Competitive indicator methods for the analysis of citrate using colorimetric assays

McCleskey, Shawn C.; Metzger, Axel; Simmons, Chris; Anslyn, Eric V.

doi:10.1016/s0040-4020(01)01093-6

Cited by 56 publications

(18 citation statements)

References 37 publications

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“…Moreover, these organic acids have similar structures, p K a values, and chromatographic behavior, further complicating the analysis. Some methods have been developed for analyzing organic acids in a wide variety of matrices, including various separation and detection methods . A commonly used technique is GC–MS, which requires sample derivatization to increase the volatility and complicated sample preparation such as protein precipitation (PPT), LLE, or SPE.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages

Zhang

et al. 2016

J of Separation Science

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A method using high-performance liquid chromatography coupled with tandem mass spectrometry was developed for the simultaneous determination of organic acids in microalgae. o-Benzylhydroxylamine was used to derivatize the analytes, and stable isotope-labeled compounds were used as internal standards for precise quantification. The proposed method was evaluated in terms of linearity, recovery, matrix effect, sensitivity, and precision. Linear calibration curves with correlation coefficients >0.99 were obtained over the concentration range of 0.4-40 ng/mL for glycolic acid, 0.1-10 ng/mL for malic acid and oxaloacetic acid, 0.02-2 ng/mL for succinic acid and glyoxylic acid, 4-400 ng/mL for fumaric acid, 20-2000 ng/mL for isocitric acid, 2-200 ng mL for citric acid, 100-10000 ng mL for cis-aconitic acid, and 1-100 ng mL for α-ketoglutaric acid. Analyte recoveries were between 80.2 and 115.1%, and the matrix effect was minimal. Low limits of detection (0.003-1 ng/mL) and limits of quantification (0.01-5 ng/mL) were obtained except cis-aconitic acid. Variations in reproducibility for standard solution at three different concentrations levels were <9%. This is the first report of the simultaneous analysis of ten organic acids in microalgae, which promotes better understanding of their growth state and provides reference value for high-yield microalgae cultures.

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Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages

Zhang

et al. 2016

J of Separation Science

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“…In order to prepare optimized fluoride sensors, several classes of anion receptors with amide, urea, thiourea or hydroxyl groups (hydrogen bond donors) and oxadiazole moieties were explored and extensively studied (4,5). Currently, structurally simple molecules containing imidazole rings have been investigated and they formed excellent host-guest complexes with fluoride anions (3).…”

Section: Introductionmentioning

confidence: 99%

Selective signaling of fluoride anion based on imidazole moieties

2011

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The imidazole ring unit in both 2-(2'-hydroxyphenyl)-benzimidazole (ligand a) and a europium(III) complex exhibited specific luminescent responses in the presence of fluoride anions. UV-visible and (1) H-nuclear magnetic resonance spectra showed that the NH bond of the imidazole ring can form a hydrogen bond with added fluoride anions. The detection limits are 5 × 10(-6) m for organic ligand and 1.0 × 10(-6) m for the europium complex respectively. The response times are less than 3 s. The europium complex exhibits a linear response in a concentration range lower than 1.0 × 10(-6) m (Y = -666.86X + 730.1).

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“…Both solids were exhaustively washed and dried (40 % yield). Solid S2 was then converted to solid S2 ‐ 1 by loading with the dye Methylthymol blue ( 1 ), which contains four carboxy groups and is expected to form strong complexes with guanidinium groups 17. In a similar manner, solid S3 was loaded with azoic dye 2 to give solid S3 ‐ 2 18.…”

Section: Methodsmentioning

confidence: 99%

Host Solids Containing Nanoscale Anion‐Binding Pockets and Their Use in Selective Sensing Displacement Assays

et al. 2005

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Competitive indicator methods for the analysis of citrate using colorimetric assays

Cited by 56 publications

References 37 publications

Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages

Simultaneous analysis of ten low‐molecular‐mass organic acids in the tricarboxylic acid cycle and photorespiration pathway in Thalassiosira pseudonana at different growth stages

Selective signaling of fluoride anion based on imidazole moieties

Host Solids Containing Nanoscale Anion‐Binding Pockets and Their Use in Selective Sensing Displacement Assays

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