Competitive fluorescence immunoassay for the rapid qualitative screening and accurate quantitative analysis of ketamine

Cao, Jie; Li, Mingjie; Chen, Xiaoying

doi:10.1039/d2ra05202d

Cited by 4 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The advantages of FIA are high selectivity and sensitivity, but the disadvantages include long incubation times and complex sample preparation. For example, Cao et al 83 reported a competitive fluorescence immunoassay consisting of a ketamine hapten and a fluorescein isothiocyanate-labelled antibody for detecting ketamine in human urine, blood, or sewage, which showed high specificity to ketamine versus a range of drugs and a LOD of 0.1 pg mL −1 . However, the method required a 2 hour sample incubation and multiple reagents.…”

Section: Luminescence Detection Of Illicit Drugs In Solutionmentioning

confidence: 99%

“…In addition to aptameric sensors, fluorescence immunoassays (FIA) have been reported for selectively detecting drugs in biofluids. [80][81][82][83] The general approach for using an FIA sensor is to have a fluorophore-labelled drug antibody that specifically binds with the target drug, which leads to a change in the fluorophore emission. The advantages of FIA are high selectivity and sensitivity, but the disadvantages include long incubation times and complex sample preparation.…”

Section: Aptamer and Biomimetic-based Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Luminescence-based detection and identification of illicit drugs

Chen

Burn

Shaw

2023

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

show abstract

Section: Luminescence Detection Of Illicit Drugs In Solutionmentioning

confidence: 99%

Section: Aptamer and Biomimetic-based Sensorsmentioning

confidence: 99%

Luminescence-based detection and identification of illicit drugs

Chen

Burn

Shaw

2023

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

show abstract

“…Some examples of these techniques are fluorescence-enabled electrochemical microscopy and competition-enabled imaging technique with super-resolution methods. [131][132][133] In a recent study, Mao et al demonstrated the capabilities of a new imaging technique for catalytic studies, which is referred as competition-enabled imaging technique (COMPEITS). 134 This tool allowed for the imaging of non-fluorescent surface reactions with a competition component where both fluorescent and non-fluorescent molecules compete to bind to a catalyst which traditionally propels a fluorescent reaction (Figure 4).…”

Section: Emerging Techniquesmentioning

confidence: 99%

“…Several methods have already been implemented for catalytic and electrochemical reaction monitoring. Some examples of these techniques are fluorescence‐enabled electrochemical microscopy and competition‐enabled imaging technique with super‐resolution methods 131–133 …”

Section: Brief Introduction To Conventional Single‐molecule Fluoresce...mentioning

confidence: 99%

Single‐molecule fluorescence microscopy for imaging chemical reactions: Recent progress and future opportunities for advancing polymer systems

Dunn,

Valdez,

Qiang

2023

Journal of Polymer Science

View full text Add to dashboard Cite

Single‐molecule fluorescence (smFL) imaging techniques have evolved greatly over the past two decades to encompass the ability to monitor chemical reactions, providing unique advantages of non‐invasive sample preparation and characterization, labeling specificity, and high spatial and temporal resolutions. This work summarizes the recent progress in this important area by first providing a brief overview of different smFL techniques, including their common optical setups and working principles. We then introduce recent developments of smFL to characterize various model chemical reaction systems, such as biochemical synthesis, catalyzed systems, and nanomaterial assembly. Furthermore, several representative areas of using smFL to understand polymer reactions are discussed, including understanding interfacial phenomenon and polymerization kinetics, as well as characterizing electrochemical reactions. We also highlight the outlook of this exciting field and potential opportunities for further development and application of smFL to enable advances in polymer chemistry and physics.

show abstract

“…Fluorescence immunoassay adalah metode yang menggabungkan spesifisitas reaksi imunologis dengan sensitivitas teknik fluoresen (Cao et al, 2022).…”

Section: Pembahasanunclassified

ERBANDINGAN NILAI C-REACTIVE PROTEIN METODE aglutinasi Lateks DAN METODE FLUORESCENCE IMMUNOASSAY (FIA) PADA PASIEN PRA SECTIO

Nurhidayanti

2023

maskermedika

View full text Add to dashboard Cite

Introduction: Examination of C-Reactive Protein (CRP) levels in the blood is a marker of the acute phase in humans which is useful for the diagnosis and monitoring of inflammatory diseases. The Latex Agglutination method is often used to obtain quick and simple results as a screening test for elevated CRP levels. Currently, a quantitative CRP examination is available in order to measure CRP levels more quickly. Fluorescene Immunoassay (FIA) is a method that has improved sensitivity and precision so that it can detect low CRP concentrations. This study aims to determine differences in C-Reactive Protein (CRP) values of the Latex Agglutination method and the FIA method in patients at Bunda Palembang Hospital in 2023. Method: Cross Sectional. The research was conducted in January 2023 at Bunda Palembang Hospital with a total sample of 38 samples. Results: The average value of C-Reactive Protein using the Latex Agglutination method was 6.6 mg/L and the FIA method was 7.0 mg/L. Data analysis of the Latex Agglutination method showed that there were differences between the FIA method and Asymp. Sig. (2-tailed) is 0.029. The sensitivity and specificity were 73.91% and 100% for the Latex Agglutination method, and 100% and 71.43% for the FIA method, respectively. Conclusion: There are differences in the C-Reactive Protein Value examination by the Latex Agglutination Method and the Fluorescene Immunoassay (FIA) Method..

show abstract

Competitive fluorescence immunoassay for the rapid qualitative screening and accurate quantitative analysis of ketamine

Abstract: In this paper, a sensitive and specific competitive fluorescence immunoassay (CFIA) method was developed and a novel competitive model was used for rapid and indirect quantitative analysis of ketamine (KET) in human urine, blood, or sewage.

Cited by 4 publications

References 46 publications

Luminescence-based detection and identification of illicit drugs

Luminescence-based detection and identification of illicit drugs

Single‐molecule fluorescence microscopy for imaging chemical reactions: Recent progress and future opportunities for advancing polymer systems

ERBANDINGAN NILAI C-REACTIVE PROTEIN METODE aglutinasi Lateks DAN METODE FLUORESCENCE IMMUNOASSAY (FIA) PADA PASIEN PRA SECTIO

Contact Info

Product

Resources

About