Competitive endogenous RNA network: potential implication for systemic lupus erythematosus

Li, Lian-Ju; Zhao, Wei; Tao, Sha‐Sha; Leng, Rui‐Xue; Fan, Yin‐Guang; Pan, Hai‐Feng; Ye, Dong‐Qing

doi:10.1080/14728222.2017.1319938

Cited by 63 publications

(44 citation statements)

References 121 publications

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“…Therefore, it is of great significance to further explore the relationship between the changes in new gene function and the development and malignant characteristics of glioma tumours, to reveal the precise molecular mechanism of its occurrence and development, to develop reasonable therapeutic drugs and determine the prognosis and to further improve the treatment of glioma and the prognosis of glioma patients. 22 In addition, lncRNA, as ceRNA, plays an important role in carcinogenesis. 21 A number of studies have shown that lncRNAs can participate in the regulation of various biological processes through ceRNA mechanism, such as muscle differentiation, cell resistance, neural cell differentiation, etc.…”

Section: Discussionsmentioning

confidence: 99%

“…21 A number of studies have shown that lncRNAs can participate in the regulation of various biological processes through ceRNA mechanism, such as muscle differentiation, cell resistance, neural cell differentiation, etc. 22 In addition, lncRNA, as ceRNA, plays an important role in carcinogenesis. 23 For example, in ovarian cancer, lncRNA HOST2 upregulates the expression of HMGA2 and c-MYC by the competitive binding with let-7b-5p, thereby promoting the occurrence and development of ovarian cancer.…”

Section: Discussionsmentioning

confidence: 99%

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FER1L4/miR‐372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells

Liang

Nie

Wang

et al. 2019

J Cellular Molecular Medi

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Long non‐coding RNAs have recently become a key regulatory factor for cancers, whereas FER1L4, a newly discovered long non‐coding RNA, has been mostly studied in gastric carcinoma and colon cancer cases. The functions and molecular mechanism of FER1L4 have been rarely reported in glioma malignant phenotypes. In this study, it was found that the expression of LncRNA FER1L4 is upregulated in high‐grade gliomas than in low‐grade cases and that a high expression of LncRNA FER1L4 predicts poor prognosis of gliomas. Meanwhile, in vitro study suggests that expression of FER1L4 with SiRNA knockdown obviously suppresses cell cycle and proliferation. It is further demonstrated by experiments that the FER1L4 knockdown suppresses growth of in vivo glioma. Besides, it is found in our study that LncRNA FER1L4 expression is positively correlated with E2F1 mRNA expression. After knockdown of FER1L4 expression, E2F1 expression is significantly down‐regulated, whereas the expression of miR‐372 is significantly up‐regulated; the up‐regulation of miR‐372 leads to significant down‐regulation of FER1L4 and E2F1 expression. In addition, it is also found that FER1L4 can be used as competitive endogenous RNA to interact or bind with miR‐371 and thereby up‐regulate E2F1, thus promoting the cycle and proliferation of glioma cells. It may be one of the molecular mechanisms in which FER1L4 plays its oncogene‐like role in gliomas.

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Section: Discussionsmentioning

confidence: 99%

Section: Discussionsmentioning

confidence: 99%

FER1L4/miR‐372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells

Liang

Nie

Wang

et al. 2019

J Cellular Molecular Medi

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“…39 We concerned that LINC00094 was up-regulated in Abeta 1-42 -in- Emerging evidence indicated that certain lncRNAs can served as a competitive endogenous RNA ("ceRNA") to regulate downstream gene expression and biological function. 43 The results of Dual-luciferase reporter assay and RIP assay showed that miR-224-5p (miR-497-5p) was enriched by LINC00094 and sponge LINC0094 in a sequence-specific manner, respectively. 41 TGFB2-OT1 regulates autophagy in vascular endothelial cells (VECs) via sponging miR-3960, miR-4488, miR-4459.…”

Section: Discussionmentioning

confidence: 99%

“…MRE has been identified to be a highly conserved sequence and used as a new language to explore ceRNA regulation network. 43 The results of Dual-luciferase reporter assay and RIP assay showed that miR-224-5p (miR-497-5p) was enriched by LINC00094 and sponge LINC0094 in a sequence-specific manner, respectively. These results supported the hypothesis that LINC00094 regulates BBB permeability in AD microenvironment.via sponging miR-224-5p/miR-497-5p.…”

Section: Discussionmentioning

confidence: 99%

The role of LINC00094/miR‐224‐5p (miR‐497‐5p)/Endophilin‐1 axis in Memantine mediated protective effects on blood‐brain barrier in AD microenvironment

Zhu

Miao

et al. 2019

J Cellular Molecular Medi

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The dysfunction of the blood‐brain barrier ( BBB ) is one of the main pathological features of Alzheimer's disease ( AD ). Memantine ( MEM ), an N ‐methyl‐ d ‐aspartate ( NMDA ) receptor antagonist, has been reported that been used widely for AD therapy. This study was performed to demonstrate the role of the MEM in regulating BBB permeability in AD microenvironment as well as its possible mechanisms. The present study showed that LINC 00094 was dramatically increased in Abeta 1‐42 ‐incubated microvascular endothelial cells ( EC s) of BBB model in vitro. Besides, it was decreased in MEM ‐incubated EC s. Silencing LINC 00094 significantly decreased BBB permeability, meanwhile up‐regulating the expression of ZO ‐1, occludin and claudin‐5. Furthermore, silencing LINC 00094 enhance the effect of MEM on decreasing BBB permeability in AD microenvironment. The analysis of the mechanism demonstrated that reduction of LINC 00094 inhibited Endophilin‐1 expression by up‐regulating miR‐224‐4p/miR‐497‐5p, promoted the expression of ZO ‐1, occludin and claudin‐5, and ultimately alleviated BBB permeability in AD microenvironment. Taken together, the present study suggests that the MEM / LINC 00094/miR‐224‐5p (miR‐497‐5p)/Endophilin‐1 axis plays a crucial role in the regulation of BBB permeability in AD microenvironment. Silencing LINC 00094 combined with MEM provides a novel target for the therapy of AD .

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“…For instance, up to 65 731 circRNAs are detected in human brain samples and neuronal circRNAs show significant conservation in both expression and sequence . As for the physiological function of circRNAs, they can serve as miRNA sponges and thereby sequester miRNAs from their natural mRNA targets . For example, Sry and ciRS‐7 can serve as sponges of miR‐138 and miR‐7, respectively .…”

Section: Introductionmentioning

confidence: 99%

Circular RNA expression profile and potential function of hsa_circ_0045272 in systemic lupus erythematosus

Zhu

Zhao

et al. 2018

Immunology

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Circular RNAs (circRNAs) represent a class of non-coding RNAs that form covalently closed RNA circles with extensive expression and conservation in mammals. Circular RNAs regulate gene expression through acting as competitive endogenous RNAs (ceRNAs) and modulating gene transcription. Accumulating evidence supports the implication of circRNAs in a variety of human diseases, but studies of circRNA role in systemic lupus erythematosus (SLE) are lacking. The present study measured the circRNA expression profiles in T cells from patients with SLE and healthy controls with human circRNA microarray and identified 127 differentially expressed circRNAs in SLE patients. Down-regulation of hsa_circ_0045272 in SLE T cells was verified with quantitative PCR. Jurkat cells with stable hsa_circ_0045272 knockdown were generated using specific lentiviral short hairpin RNA for functional studies. Flow cytometric analysis indicated that hsa_circ_0045272 knockdown significantly up-regulated the early apoptosis of Jurkat cells. Meanwhile, ELISA showed that hsa_circ_0045272 knockdown significantly enhanced interleukin-2 production of activated Jurkat cells. Then, ceRNAs were predicted for hsa_circ_0045272 and the significant down-regulation of two mRNAs predicted as ceRNAs, NM_003466 (PAX8) and NM_015177 (DTX4), but not their corresponding proteins, was validated. Furthermore, dual luciferase reporter assay indicated binding of hsa_circ_0045272 with hsa-miR-6127. Circular RNA-mRNA co-expression networks showed the correlation of circRNAs with mRNAs and provided additional clues to circRNA functions. Our study demonstrated dysregulated circRNAs in SLE and revealed the function of hsa_circ_0045272 in negatively regulating apoptosis and interleukin-2 secretion and its potential mechanism. The implication of hsa_circ_0045272 and other abnormal circRNAs in SLE merits further investigation.

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Competitive endogenous RNA network: potential implication for systemic lupus erythematosus

Cited by 63 publications

References 121 publications

FER1L4/miR‐372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells

FER1L4/miR‐372/E2F1 works as a ceRNA system to regulate the proliferation and cell cycle of glioma cells

The role of LINC00094/miR‐224‐5p (miR‐497‐5p)/Endophilin‐1 axis in Memantine mediated protective effects on blood‐brain barrier in AD microenvironment

Circular RNA expression profile and potential function of hsa_circ_0045272 in systemic lupus erythematosus

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