Abstract:Background
PSMA-targeted radionuclide therapy (TRT) is a promising treatment for prostate cancer (PCa), but dose-limiting xerostomia can severely limit its clinical adaptation, especially when using alpha-emitting radionuclides. With [18F]DCFPyL as a surrogate for PSMA-TRT, we report a novel method to selectively reduce salivary gland (SG) uptake of systemically administered [18F]DCFPyL by immediate prior infusion of non-radioactive standard of [18F]DCFPyL (DCFPyL) directly into the SG via retr… Show more
“…However, adding the albumin-binding motif to the bivalent ligand impaired its PSMA binding capability, with the IC 50 of DOTA-Alb-Bi-PSMA reduced to 8.68 ± 3.10 nM. , All PSMA ligands were radiolabeled with gallium-68 and lutetium-177 and exhibited good stability in PBS and human serum. It has been reported that self-blocking of PSMA ligands can significantly affect the distribution of the radioligands in vivo; ,− therefore, in this study, the 177 Lu-labeled ligands were purified by HPLC for further study.…”
Recently, we developed a bivalent prostate-specific membrane antigen (PSMA) radioligand ([ 18 F]AlF-Bi-PSMA), which showed higher tumor uptake and retention in PSMA-positive mouse models than the clinically used radioligands, [ 68 Ga]Ga-PSMA-11 and [ 18 F]PSMA-1007. Here, we developed two 177 Lu-labeled bivalent PSMA ligands with (DOTA-Alb-Bi-PSMA) or without an albumin-binding motif (DOTA-Bi-PSMA) to enhance radiotherapeutic efficacy with minimal toxicity. The results demonstrated that both 177 Lu-labeled bivalent radioligands showed good stability, high binding affinity, and PSMA-targeting specificity in vitro. Compared with [ 177 Lu]Lu-PSMA-617, both [ 177 Lu]Lu-Bi-PSMA and [ 177 Lu]Lu-Alb-Bi-PSMA showed a higher area under the curve (AUC) of tumor accumulation and superior therapeutic efficacy. However, [ 177 Lu]Lu-Alb-Bi-PSMA exhibited a dose-dependent increase in acute damage to kidneys. In terms of the radionuclide therapy efficacy and side effects, [ 177 Lu]Lu-Bi-PSMA exhibited well-balanced action with high tumor-to-organs AUC ratios, resulting in remarkable therapeutic efficacy and negligible side effects. These promising results warrant further investigations to achieve the clinical translation of [ 177 Lu]Lu-Bi-PSMA.
“…However, adding the albumin-binding motif to the bivalent ligand impaired its PSMA binding capability, with the IC 50 of DOTA-Alb-Bi-PSMA reduced to 8.68 ± 3.10 nM. , All PSMA ligands were radiolabeled with gallium-68 and lutetium-177 and exhibited good stability in PBS and human serum. It has been reported that self-blocking of PSMA ligands can significantly affect the distribution of the radioligands in vivo; ,− therefore, in this study, the 177 Lu-labeled ligands were purified by HPLC for further study.…”
Recently, we developed a bivalent prostate-specific membrane antigen (PSMA) radioligand ([ 18 F]AlF-Bi-PSMA), which showed higher tumor uptake and retention in PSMA-positive mouse models than the clinically used radioligands, [ 68 Ga]Ga-PSMA-11 and [ 18 F]PSMA-1007. Here, we developed two 177 Lu-labeled bivalent PSMA ligands with (DOTA-Alb-Bi-PSMA) or without an albumin-binding motif (DOTA-Bi-PSMA) to enhance radiotherapeutic efficacy with minimal toxicity. The results demonstrated that both 177 Lu-labeled bivalent radioligands showed good stability, high binding affinity, and PSMA-targeting specificity in vitro. Compared with [ 177 Lu]Lu-PSMA-617, both [ 177 Lu]Lu-Bi-PSMA and [ 177 Lu]Lu-Alb-Bi-PSMA showed a higher area under the curve (AUC) of tumor accumulation and superior therapeutic efficacy. However, [ 177 Lu]Lu-Alb-Bi-PSMA exhibited a dose-dependent increase in acute damage to kidneys. In terms of the radionuclide therapy efficacy and side effects, [ 177 Lu]Lu-Bi-PSMA exhibited well-balanced action with high tumor-to-organs AUC ratios, resulting in remarkable therapeutic efficacy and negligible side effects. These promising results warrant further investigations to achieve the clinical translation of [ 177 Lu]Lu-Bi-PSMA.
“…Nevertheless, long-term salivary gland toxicity needs to be investigated. A recent pilot study investigated the use of cold DCFPyL instillation into the salivary glands in order to decrease salivary uptake, mitigating xerostomia in patients scheduled for radioligand therapy [178]. Numerous studies have recently evaluated the application of Cu as PET/TC radiotracer in oncology because of its capacity to act as diagnostic and theragnostic radiotracer.…”
Section: Conclusion and Future Perspectivesmentioning
Prostate cancer is the most frequent epithelial neoplasia after skin cancer in men starting from 50 years and prostate-specific antigen (PSA) dosage can be used as an early screening tool. Prostate cancer imaging includes several radiological modalities, ranging from ultrasonography, computed tomography (CT), and magnetic resonance to nuclear medicine hybrid techniques such as single-photon emission computed tomography (SPECT)/CT and positron emission tomography (PET)/CT. Innovation in radiopharmaceutical compounds has introduced specific tracers with diagnostic and therapeutic indications, opening the horizons to targeted and very effective clinical care for patients with prostate cancer. The aim of the present review is to illustrate the current knowledge and future perspectives of nuclear medicine, including stand-alone diagnostic techniques and theragnostic approaches, in the clinical management of patients with prostate cancer from initial staging to advanced disease.
“…A recent preclinical study also reported on direct retrograde installation of the nonradioactive standard of [ 18 F]DCFPyL, that is DCFPyL, into the salivary glands, potentially decreasing salivary uptake. Such blocking experiments may pave the way to mitigate xerostomia in a clinical setting, e. g., in patients scheduled for RLT [55]. Last, in patients scheduled for RLT, PET/CT-based parameters at baseline may predict early biochemical response and overall survival [56,57].…”
Prostate-specific membrane antigen (PSMA)-directed positron emission tomography (PET) has gained increasing interest for imaging of men affected by prostate cancer (PC). In recent years, 68Ga-labeled PSMA compounds have been widely utilized, although there is a trend towards increased utilization of 18F-labeled agents. Among others, [18F]DCFPyL (piflufolastat F 18, PYLARIFY) has been tested in multiple major trials, such as OSPREY and CONDOR, which provided robust evidence on the clinical utility of this compound for staging, restaging, and change in management. Recent explorative prospective trials have also utilized [18F]DCFPyL PET/CT for response assessment, e.g., in patients under abiraterone or enzalutamide, rendering this 18F-labeled PSMA radiotracer as an attractive biomarker for image-guided strategies in men with PC. After recent approval by the U.S. Food and Drug Administration, one may expect more widespread use, not only in the U.S., but also in Europe in the long term. In the present review, we will provide an overview of the current clinical utility of [18F]DCFPyL in various clinical settings for men with PC.
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