Competitive abilities of common field isolates and a commercial strain of Rhizobium leguminosarum bv. trifolii for clover nodule occupancy

Denton, Matthew D.; Reeve, Wayne; Howieson, John; Coventry, David

doi:10.1016/s0038-0717(03)00146-9

Cited by 33 publications

(19 citation statements)

References 44 publications

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“…The values of k were found to vary according to the experiment (Amarger 1984); the extreme values were 0.15 and 1.22 but most of the values were between 0.3 and 0.5. Denton et al (2003) calculated k for four Rhizobium leguminosarum bv. trifolii inoculation strains in a competition experiment, and the values varied between 0.693-1.551.…”

Section: Resultsmentioning

confidence: 99%

“…Various inoculation methods have been used in experiments determining Rhizobium leguminosarum bv. trifolii strains competitiveness: (i) repeated and increased inoculation with single strain culture (Mårtensson 1989), (ii) preparation of single or mixed strain peat-based inoculants (Svenning et al 2001) or (iii) inoculation with slurry containing different concentrations of marked rhizobia (Denton et al 2003). Amarger and Lobreu (1982) and Amarger (1984) presented an excellent model for studying the competition problem based on the fact that the percentage of nodules formed by the inoculation strain is related to the logarithm of the number of bacteria in the inoculum.…”

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confidence: 99%

“…Rhizobial strain is thus more competitive than others if the number of nodules it has occupied is higher than its proportional representation in the inoculum. Later, this model was modified (Beattie et al 1989) and experimentally verified for various rhizobia (Šimon et al 1996, Sessitsch et al 1997, Denton et al 2003. Rapid and precise identification methods are presently required for studying competition between microorganisms in soil however.…”

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confidence: 99%

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New Rhizobium leguminosarum bv. trifolii isolates: Evaluation of competitiveness for clover nodule occupancy

Šimon¹,

Salava²

2006

PLANT SOIL ENVIRON

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An interrelationship between introduced and indigenous rhizobia focused on their competitiveness in nodulation was evaluated in a soil pot experiment. Clover seeds were inoculated by six different gradual concentrations of inoculum of two effective Rhizobium leguminosarum bv. trifolii isolates (inoculation strains 1/2 and 14/2). At the beginning of flowering, clover plants were removed from the pots, and nodules from each pot representing different degrees of inoculum level were taken for reisolate cultivation. The PCR technique was used for the identification of rhizobial reisolates, random amplified polymorphic DNA product patterns were acquired and analysed. Nodule occupation by inoculation strains or indigenous Rhizobium leguminosarum bv. trifolii strains was assessed by comparing the number of nodules formed by inoculation or indigenous strains and inoculation strains competitiveness was calculated. Nodule occupancy by the inoculation strain 1/2 increased sharply with the increase in inoculum dose, whereas in inoculation strain 14/2 more nodules were formed gradually starting from low inoculum level. Competitiveness of inoculation strain 1/2 was calculated as low and was documented by an absence of nodule occupancy in four inoculation levels. On the other hand, competitiveness of the inoculation strain 14/2 was considerably higher, and even in low inoculum dose this strain was more competitive than native rhizobia. Although both the inoculation strains 1/2 and 14/2 were found highly efficient in nitrogen fixation, only the strain 14/2 was able to manifest this characteristic due to the higher competitiveness when applied in lower doses.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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New Rhizobium leguminosarum bv. trifolii isolates: Evaluation of competitiveness for clover nodule occupancy

Šimon¹,

Salava²

2006

PLANT SOIL ENVIRON

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“…Secondly, they may have resided intercellularly in the nodule tissue and were protected from the sterilant (Barreto et al, 2012;Dudeja et al, 2012). Thirdly, they may have entered the nodule as co-inoculants with other nodulating strains (Bai et al, 2003;Denton et al, 2003;Dudeja et al, 2012;Egamberdieva et al, 2010;Sturz et al, 1997). Finally they may be rhizobial strains which have lost the capacity to nodulate upon sub-culture (Howieson et al, 2000b;Sachs et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos

Howieson

Meyer

Vivas-Marfisi

et al. 2013

Soil Biology and Biochemistry

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“…Castro et al (1999) found that peanut roots have been well nodulated by native strain rather than the exotic one. Hence, selection of efficient strains based on their local ecologic adaptation can increase grain production of crops (Denton et al 2003;Lindström et al 2010;Bianco et al 2013). On top of differing the rhizobia in their ability to fix N 2 , the effective peanut-rhizobia symbiosis is the product of rhizobial and plant cultivars as well as of environmental conditions (Nelson 1987;Sessitsch et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Development of environmental friendly bioinoculate for peanut (Arachis hypogea L.) production in Eastern Ethiopia

Argaw

2017

Environ Syst Res

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Background: Bacterial bioinoculate is one of environmentally friendly input for crop production. Several works revealed that effectiveness of Rhizobium bioinoculant have been affected by the native rhizobia population. Hence, this study was initiated to evaluate the effect of selected Bradyrhizobium in nodulating peanut in the major growing areas of Ethiopia. Methods:The effect of eight effective Bradyrhziobium vis-a-vis 20 kg N ha −1 and the control check on the nodulation and yield of two peanut cultivars (Sedi and Werer 942) at Babile and Fedis, eastern Ethiopia, during 2013 cropping season was evaluated. Results:The results showed that the inoculation, location and cultivar and their two and three ways interaction had a significant influence on the nodulation and grain yield. Inoculation of Bradyrhizobium increased the nodule number, nodule dry weight, number of seeds per plants, total pods weight, hundred seeds weight, total biomass yield, grain yield and shelling % up to 37, 24.9, 22.4, 22.5, 5.5, 26.2, 35.8 and 24.1%, respectively. Isolate HUGNR-29 was found to be more effective as it scored the highest nodule dry weight and grain yield. Application of inorganic N increased the yield and yield components of peanut in either of locations. Though the statistically better effective nodule % was recorded at Babile, significantly higher nodule number, nodule dry weight, and total N accumulation were obtained from Fedis than Babile site. Significantly higher total pods weight, hundred seeds weight, total biomass weight, grain yield and total plant N accumulation was recorded with the cultivar werer-962 than those produced with Bati-Sedi. Conclusions:The result showed that Bradyrhizobium inoculation is essential to improve the peanut production through environmentally and economically sustainable ways though the soil native rhizobia nodulating peanut was high. Peanut cultivar and study site based rhizobia inoculant development is also important.

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Competitive abilities of common field isolates and a commercial strain of Rhizobium leguminosarum bv. trifolii for clover nodule occupancy

Cited by 33 publications

References 44 publications

New Rhizobium leguminosarum bv. trifolii isolates: Evaluation of competitiveness for clover nodule occupancy

New Rhizobium leguminosarum bv. trifolii isolates: Evaluation of competitiveness for clover nodule occupancy

Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos

Development of environmental friendly bioinoculate for peanut (Arachis hypogea L.) production in Eastern Ethiopia

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