Competition between isogenic mutS and mut + populations of Escherichia coli K12 in continuously growing cultures

Tröbner, Wolfgang; Piechocki, Rudolf

doi:10.1007/bf00328719

Cited by 34 publications

(26 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such experiments have primarily been performed in vitro in E. coli (Trobner and Piechocki, 1984a;Labat et al, 2005;de Visser and Rozen, 2006;Loh et al, 2010;Gentile et al, 2011) and the yeast Saccharomyces cerevisiae (Thompson et al, 2006;Raynes et al, 2011Raynes et al, , 2012Raynes et al, , 2014, but some work has also been carried out in vivo by propagating E. coli populations in the mouse gut (Giraud et al, 2001). The focus of these experiments has not necessarily been to demonstrate mutator hitchhiking anew, but rather to explore the influence of various population genetic factors on the likelihood and dynamics of mutator hitchhiking, as discussed below.…”

Section: Mutator Hitchhiking As a Fundamental Aspect Of Asexual Populmentioning

confidence: 99%

Experimental evolution and the dynamics of genomic mutation rate modifiers

Raynes

Sniegowski

2014

Heredity

View full text Add to dashboard Cite

Because genes that affect mutation rates are themselves subject to mutation, mutation rates can be influenced by natural selection and other evolutionary forces. The population genetics of mutation rate modifier alleles has been a subject of theoretical interest for many decades. Here, we review experimental contributions to our understanding of mutation rate modifier dynamics. Numerous evolution experiments have shown that mutator alleles (modifiers that elevate the genomic mutation rate) can readily rise to high frequencies via genetic hitchhiking in non-recombining microbial populations. Whereas these results certainly provide an explanatory framework for observations of sporadically high mutation rates in pathogenic microbes and in cancer lineages, it is nonetheless true that most natural populations have very low mutation rates. This raises the interesting question of how mutator hitchhiking is suppressed or its phenotypic effect reversed in natural populations. Very little experimental work has addressed this question; with this in mind, we identify some promising areas for future experimental investigation.

show abstract

Section: Mutator Hitchhiking As a Fundamental Aspect Of Asexual Populmentioning

confidence: 99%

Experimental evolution and the dynamics of genomic mutation rate modifiers

Raynes

Sniegowski

2014

Heredity

View full text Add to dashboard Cite

show abstract

“…Anti-mutator mutants with lower than normal mutation rates have also been observed (reviewed by Sniegowski et al, 2000), albeit at much lower frequencies than mutators (Drake et al, 1998). However, within mutator populations, mutants with lower mutation rates (but higher than wild-type) can evolve (Tro$ bner & Piechocki, 1984 ;Giraud et al, 2001a). These studies show that evolution of the mutation rate is possible in evolving populations of bacteria.…”

Section: Mutation Rate Evolutionmentioning

confidence: 99%

“…However, the evidence for such direct fitness benefits is at best indirect (reviewed by Sniegowski et al, 2000). Moreover, direct competitions between mutators and their repair-proficient equivalents reveal a small growth rate disadvantage for mutators Tro$ bner & Piechocki, 1984 ;Boe et al, 2000), presumably due to the increased production of deleterious mutations in their offspring. The magnitude of this direct cost depends on mutator strength (Drake, 1991 ;Tenaillon et al, 1999).…”

Section: Short-term Fatementioning

confidence: 99%

“…Denamur et al, 2000 ;Brown et al, 2001). Provided they do arise in the population, the direct fitness advantage of DNA-repair-proficient mutants -and hence the chance that they take over the population -is still small Tro$ bner & Piechocki, 1984 ;Boe et al, 2000). The probability that a population will quickly lose its mutator phenotype once it has been fixed is thus not very high.…”

Section: Long-term Fatementioning

confidence: 99%

See 1 more Smart Citation

The fate of microbial mutators

Visser

2002

Microbiology

View full text Add to dashboard Cite

“…As for microbes, expanding offspring genome diversities by elevating replication mutation rates to achieve rapid adaptation and competitive advantages in harsh environments has been discovered and verified both in vivo [40][41][42] and in silico [43,44]. Various natural mutator genes have been isolated and analyzed.…”

Section: Discussionmentioning

confidence: 99%

Genome Replication Engineering Assisted Continuous Evolution (GREACE) to Improve Microbial Tolerance for Biofuels Production

Luan¹,

Zhang²,

Li³

et al. 2015

New Microbial Technologies for Advanced Biofuels

View full text Add to dashboard Cite

Background: Microbial production of biofuels requires robust cell growth and metabolism under tough conditions. Conventionally, such tolerance phenotypes were engineered through evolutionary engineering using the principle of "Mutagenesis followed-by Selection". The iterative rounds of mutagenesis-selection and frequent manual interventions resulted in discontinuous and inefficient strain improvement processes. This work aimed to develop a more continuous and efficient evolutionary engineering method termed as "Genome Replication Engineering Assisted Continuous Evolution" (GREACE) using "Mutagenesis coupled-with Selection" as its core principle. Results: The core design of GREACE is to introduce an in vivo continuous mutagenesis mechanism into microbial cells by introducing a group of genetically modified proofreading elements of the DNA polymerase complex to accelerate the evolution process under stressful conditions. The genotype stability and phenotype heritability can be stably maintained once the genetically modified proofreading element is removed, thus scarless mutants with desired phenotypes can be obtained. Kanamycin resistance of E. coli was rapidly improved to confirm the concept and feasibility of GREACE. Intrinsic mechanism analysis revealed that during the continuous evolution process, the accumulation of genetically modified proofreading elements with mutator activities endowed the host cells with enhanced adaptation advantages. We further showed that GREACE can also be applied to engineer n-butanol and acetate tolerances. In less than a month, an E. coli strain capable of growing under an n-butanol concentration of 1.25% was isolated. As for acetate tolerance, cell growth of the evolved E. coli strain increased by 8-fold under 0.1% of acetate. In addition, we discovered that adaptation to specific stresses prefers accumulation of genetically modified elements with specific mutator strengths. Conclusions: We developed a novel GREACE method using "Mutagenesis coupled-with Selection" as core principle. Successful isolation of E. coli strains with improved n-butanol and acetate tolerances demonstrated the potential of GREACE as a promising method for strain improvement in biofuels production.

show abstract

Competition between isogenic mutS and mut + populations of Escherichia coli K12 in continuously growing cultures

Cited by 34 publications

References 6 publications

Experimental evolution and the dynamics of genomic mutation rate modifiers

Experimental evolution and the dynamics of genomic mutation rate modifiers

The fate of microbial mutators

Genome Replication Engineering Assisted Continuous Evolution (GREACE) to Improve Microbial Tolerance for Biofuels Production

Contact Info

Product

Resources

About