Competition between enantiomorphs of amino acids during intestinal absorption

Jervis, E. Lesly; Smyth, David

doi:10.1113/jphysiol.1959.sp006126

Cited by 36 publications

(11 citation statements)

References 15 publications

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“…The same would also apply to the D-isomer, which can be transported, although apparently with less affinity for it, by the same system that transports the L-isomer (Jervis & Smyth, 1959;Webber, 1963). (There is, however, the anomalous finding in kidney to be explained, in which the D-isomer, but not the L-isomer, of the acidic amino acids inhibits L-histidine uptake, as shown in Fig.…”

Section: Discussionmentioning

confidence: 92%

Effect of acidic (dicarboxylic) alpha‐amino acids on uptake of L‐histidine by intestinal mucosa, testis, spleen and kidney in vitro: a comparison with effect in brain.

Neame¹

1965

The Journal of Physiology

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Section: Discussionmentioning

confidence: 92%

Effect of acidic (dicarboxylic) alpha‐amino acids on uptake of L‐histidine by intestinal mucosa, testis, spleen and kidney in vitro: a comparison with effect in brain.

Neame¹

1965

The Journal of Physiology

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“…Wiseman (1955) found that L-methionine could compete successfully with L-histidine in vitro for the transport mechanism. Agar, Hird & Sidhu (1956) demonstrated the same effect in vivo, and Jervis & Smyth (1959a) found that L-methionine could compete successfully in vivo with L-histidine and D-histidine for intestinal absorption. All these results are consistent with the low constant for Lmethionine obtained in the present experiments.…”

Section: Michaelis-menten Kineticsmentioning

confidence: 65%

The effect of concentrations of amino acids on their rate of absorption from the intestine

Jervis¹,

Smyth²,

Davies³

et al. 1959

The Journal of Physiology

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The relation between the rate of absorption of a substance from the intestine and its concentration has frequently been used as a criterion of the process involved in absorption. Hober & Hober (1937), Lathe (1943) and others have concluded that the rate of absorption of amino acids is not proportional to the concentration in the intestine, and regarded this as evidence of the presence of an active process concerned with absorption. Hetenyi & Winter (1952) found that the rate of absorption of glycine, histidine and ,B-alanine was not proportional to concentration, while in the case of proline there was a straightline relationship. The following experiments were undertaken to study more carefully the relationship between the rate of absorption and concentration of amino acids, and furthermore the D-and L-enantiomorphs of different amino acids were compared. A preliminary account has been given by Jervis & Smyth (1959b). METHODSAlbino rats were anaesthetized with pentobarbitone sodium and the procedure for study of absorption was that described by Jervis, Johnson, Sheff & Smyth (1956). By this technique it is possible to measure both the rate of absorption and also the concentration of the substance in the lumen of the intestine. The amino acids, neutralized by addition of NaOH, were dissolved in NaCl solution 09 % (w/v) and circulated through the lumen of the intestine for 20 min. At the end of this period the amount remaining in the intestine was washed out and estimated, and from this and that initially added the amount absorbed was calculated.Chemicals. The amino acids used were D-and L-methionine and histidine and were obtained commercially.

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“…It is of interest that Jervis & Smyth (1959), who examined the behaviour of D-histidine and L-methionine when the amino acids were present either singly or together in the intestinal lumen of anaesthetized rats, recorded that L-methionine decreased the rate of luminal disappearance of D-histidine. More recently, Hindmarsh et al (1966) argued that D-histidine was actively transported by normal hamster intestine, because they found that it acted as an inhibitor of sugar transport, as did amino acids which were known to be actively transported, whereas passively transported amino acids had no such effect.…”

Section: Discussionmentioning

confidence: 99%