Compensation of aberrations in holographic microscopes: main strategies and applications

Sirico, Daniele; Miccio, Lisa; Wang, Zhe; Memmolo, Pasquale; Xiao, Wen; Che, Leiping; Lu, Xin; Pan, Feng; Ferraro, Pietro

doi:10.1007/s00340-022-07798-8

Cited by 29 publications

(13 citation statements)

References 98 publications

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“…Therefore, the conventional hologram recorded by single shot often need to use numerical simulation 36 or self-reference method 37 to remove the background phase distortion. 38 These schemes are reliable when the topographic changes of the membrane are small. But when holographic recording of the membrane with large surface undulations, the features in the FoV can greatly affect the accuracy of background distortion removal.…”

Section: Resultsmentioning

confidence: 99%

“…When the imaging FoV is at a higher magnification (≥20×), the unavoidable problem of single shot is the FoV shrinkage. Therefore, the conventional hologram recorded by single shot often need to use numerical simulation 36 or self-reference method 37 to remove the background phase distortion 38 . These schemes are reliable when the topographic changes of the membrane are small.…”

Section: Resultsmentioning

confidence: 99%

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Multimodal quantitative phase contrast characterization of thin polymeric film

et al. 2023

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.Polymeric thin films represent an emerging industrial area driven by their enormous technological and commercial potential in interdisciplinary sectors such as chemistry, material science, engineering, and physics. The large selection in terms of materials/composites and the wide range of technological solutions that could be used for their fabrication could create confusion for the final user requiring a quantitative characterization of their properties. This analysis could be even more complex in the case of functionalized polymeric films such as the samples reported in this work. Here we present how thin polymer films can be wholly characterized by applying a multiplicity of optical methods. Films were realized by a special liquid one-step process. Moreover, such polymer films were functionalized here for the first time by mesoporous silica nanoparticles. The nanoparticles were added to a polymeric matrix. We show that a full characterization was achieved by employing three different microscope techniques, i.e., scanning electron microscope, digital holography (DH), and space-time DH. Exploiting such a multimodal methodology can be of great benefit for characterizing the functionalized polymeric thin films. In fact, multiple characterization in different conditions was possible. The results reported in terms of morphological information, thickness distribution, three-dimensional (3D) mapping, large field of view, high magnification, and super resolution of the zoomed area offer a good solution for testing materials and obtaining a quantitative characterization and whole inspection in the case of complex polymeric samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Multimodal quantitative phase contrast characterization of thin polymeric film

et al. 2023

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“…Spherical wavefront distortions must be compensated to provide accurate phase measurements across the imaged field of view, converting the non-telecentric DHM system into a linear shift-invariant phase tool 8 . These distortions can be compensated through numerical methods [7][8][9][10][11][12][13][14][15][16][17][18] . Reported computational approaches have proposed the estimation of the spherical wavefront using Zernike polynomial fitting (ZPF) 9,10 , least square surface fitting 11,12 or principal component analysis (PCA) 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Overview of the automatic reconstruction method for quantitative phase imaging using a digital holographic microscope operating in non-telecentric regime

Bogue-Jimenez

Trujillo

Doblas

2023

Quantitative Phase Imaging IX

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Digital holographic microscopy (DHM), which provides quantitative phase imaging (QPI), has been widely applied in material and biological applications. The performance of DHM technologies relies heavily on computational reconstruction methods to provide accurate phase measurements. For example, non-telecentric DHM systems should compensate for the spherical wavefront associated with a non-telecentric configuration. The size of the ±1 diffraction orders in the hologram spectrum depends inversely on the radius of the curvature of the spherical wavefront introduced by the non-telecentric DHM system. Therefore, one can estimate the radius of curvature of the spherical wavefront by analyzing the hologram spectrum. Here, we outline the steps for the automatic reconstruction of phase images without distortions and with minimum user input from a hologram recorded in a non-telecentric DHM system. The proposed reconstruction approach can be divided into six main steps. The first step automatically selects the +1 diffraction order in the hologram spectrum. Secondly, the spherical wavefront parameters and the interference angle are estimated by analyzing the size and position of the selected +1 order. The third and fourth steps are the spatial filtering of the +1 order and the compensation of the interference angle, respectively. The next step involves the estimation of the center of the spherical wavefront. Finally, there is a fine-tuning step to optimize the estimated parameters and provide a phase image with minimum phase distortions. We have identified the relevant metrics in each step, compared multiple approaches, and selected the one with the higher performance for all our experimental holograms.

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“…The sensitivity of quantitative phase reconstruction to subtle changes in the optical field makes the phase reconstruction susceptible to optical aberrations. These phase aberrations may severely hinder the quantitative measurement of the object's thickness or height with distorted visualization [7].…”

Section: Introductionmentioning

confidence: 99%

Aberration-free high-bandwidth holographic imaging

Huang,

Cao

2023

SPIE-CLP Conference on Advanced Photonics 2023

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Objective measurements of the morphology and dynamics of label-free cells and tissues can be achieved by quantitative phase with low phototoxicity and no photobleaching. Modern quantitative optical imaging possesses a huge information capacity. The morphology and dynamics of label-free tissues can be exploited by sample-induced changes in the optical field from quantitative phase imaging. Its sensitivity to subtle changes in the optical field makes the reconstructed phase susceptible to phase aberrations. We present aberration-free high-bandwidth holographic microscopy which exploits high-throughput label-free quantitative phase imaging. Firstly, a full-bandwidth holographic reconstruction is retrieved from interferograms by establishing a holographic multiplexing framework. Based on the analyticity of band-limited signal under a diffraction-limited system, the maximum space bandwidth utilization limit in a single multiplexing hologram is increased to the maximum sensor limit. Secondly, A variable sparse splitting framework on quantitative phase aberration extraction is imported based on the alternating direction aberration-free method. By formulating the aberration extraction as a convex quadratic problem, the background phase aberration can be fast and directly decomposed with the specific complete basis functions such as Zernike or standard polynomials. Faithful high throughput phase reconstruction can be obtained by eliminating global background aberration. It opens a new route to multiplexing quantitative optical imaging and helps to improve the performance of constraint-free modern optical microscopes in various spectral regimes.

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Compensation of aberrations in holographic microscopes: main strategies and applications

Cited by 29 publications

References 98 publications

Multimodal quantitative phase contrast characterization of thin polymeric film

Multimodal quantitative phase contrast characterization of thin polymeric film

Overview of the automatic reconstruction method for quantitative phase imaging using a digital holographic microscope operating in non-telecentric regime

Aberration-free high-bandwidth holographic imaging

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