“…Although it is possible to simultaneously determine polyols and trehalose by NMR (Hocking, 1986;Meikle et al, 1991;Van Eck et al, 1993), this technique is not as sensitive as HPLC and large samples are needed to perform accurate analyses (Van Eck et al, 1993). GC analyses have been used frequently in studies of fungal carbohydrates (Al-Hamdani and Cooke, 1978;Bidochka et al, 1990;Bhajekar and Kulkarni, 1992;Aoki et al, 1993). However, no GC protocol appears to be available for the simultaneous determination of different types of carbohydrates (Witteveen et al, 1995).…”