Compartmentalization of the Mitogen-Activated Protein Kinase (MAPK) in Hepatic Endosomes: Association with the Internalized Epidermal Growth Factor (EGF) Receptor

Faure, Robert; Gaulin, Jean-François; Bourgoin, Sylvain G.; Fortier, Suzanne

doi:10.1006/mcbr.1999.0120

Cited by 8 publications

(9 citation statements)

References 43 publications

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“…2B). The G/E fraction used in this study was shown to contain Golgi elements but to be essentially free of plasmalemma and other subcellular constituents (47,48,57). This was affirmed here by the detection of the slower migrating Cdk2 isoform in the cytosolic fraction, which was barely detected in the G/E and PM fractions ( Fig.…”

Section: Resultssupporting

confidence: 70%

“…Both the stacked saccules of the Golgi apparatus and the endosomes with their characteristic content of lipoprotein-like particles were observed. The endosomal fractions representing the late (Li) and early (Gi) ENs were prepared from the parent light mitochondrial (L) and microsomal (P) fractions of liver homogenates, respectively (47,48). The yield of the G/E fractions was 0.51 Ϯ 0.06 mg protein/g, liver weight (n ϭ 15) and similar to that described by Khan et al (46).…”

Section: Methodsmentioning

confidence: 60%

“…Among them we have identified with specific antibodies the EGFR (170 kDa) and its main substrate p55 Shc (55) (data not shown) as well as the 43-kDa mitogen-activated protein kinase (48). By partial proteolysis coupled to mass spectrometry, a number of other proteins, mainly proteases or protease inhibitors, 2 were also identified (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In liver parenchyma, EGF was previously shown to be internalized along with its receptor in a time-and dose-dependent manner with the maximal concentration being attained 15 min after injection of the ligand (48,60,61). In contrast to insulin, which was found to rapidly dissociate and degrade, EGF remains bound to its receptor in hepatic ENs (62).…”

Section: Effect Of Exogenously Added Cdk2 On Fusion Events In Vitro-mentioning

confidence: 99%

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Characterization of Cdk2-Cyclin E Complexes in Plasma Membrane and Endosomes of Liver Parenchyma

Gaulin¹,

Fiset²,

Fortier

et al. 2000

Journal of Biological Chemistry

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Rat liver parenchyma Golgi/endosomes fractions harbor a tyrosine-phosphorylated 34-kDa protein. Screening of Golgi, endosomes (ENs), plasmalemma (PM), and cytosolic (Cyt) fractions revealed the presence of the mitotic kinase Cdk2 in ENs, PM, and Cyt. The fluid phase endocytic marker horseradish peroxidase gained access to the endosomal Cdk2, confirming its localization. Cdk2 was shown to be associated to cyclin E and was active in ENs and PM fractions. The administration of a single dose of insulin (1.5 g/100 g, body weight) induced a time-dependent activation of the insulin receptor kinase in these structures. Insulin receptor-kinase activation was followed by the inhibition of immunoprecipitated Cdk2-cyclin E kinase activity in PM and the progressive disappearance of cyclin E. In marked contrast, no such effect was observed in ENs. The injection of a phosphotyrosyl phosphatase inhibitor (bpV-(phen)) increased the levels of cyclin E in ENs and PM. A massive recruitment of p27 kip1 was observed in the Cdk2-cyclin E complexes isolated from PM and Cyt but not from ENs. In vitro, Cdk2-cyclin E complexes have the capacity to inhibit the formation of hybrid structures containing horseradish peroxidase and radioiodinated epidermal growth factor. Therefore, in the PM and ENs of adult rat liver, an active and regulated pool of the mitotic kinase Cdk2-cyclin E and some yet to be defined effectors are present. Cdk2 may contribute to the modulation of transport events and/or maintenance of the topology of endocytic elements.

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Section: Resultssupporting

confidence: 70%

Section: Methodsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 97%

Section: Effect Of Exogenously Added Cdk2 On Fusion Events In Vitro-mentioning

confidence: 99%

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Characterization of Cdk2-Cyclin E Complexes in Plasma Membrane and Endosomes of Liver Parenchyma

Gaulin¹,

Fiset²,

Fortier

et al. 2000

Journal of Biological Chemistry

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“…In animal and yeast cells, it is well documented that the signalling specificity of MAPKs is, among others, provided by localization to specific subcellular compartments, such as the PM, Golgi, endosomes, cytoskeleton and the nucleus (Faure et al, 1999;Howe et al, 2001;Bisel et al, 2008;Inder et al, 2008). In plants, a few MAPKs like p43…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane

et al. 2009

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). † These two authors contributed equally to this work. SUMMARYThe proper spatial and temporal expression and localization of mitogen-activated protein kinases (MAPKs) is essential for developmental and cellular signalling in all eukaryotes. Here, we analysed expression, subcellular localization and function of MPK6 in roots of Arabidopsis thaliana using wild-type plants and three mpk6 knock-out mutant lines. The MPK6 promoter showed two expression maxima in the most apical part of the root meristem and in the root transition zone. This expression pattern was highly consistent with 'no root' and 'short root' phenotypes, as well as with ectopic cell divisions and aberrant cell division planes, resulting in disordered cell files in the roots of these mpk6 knock-out mutants. In dividing root cells, MPK6 was localized on the subcellular level to distinct fine spots in the pre-prophase band and phragmoplast, representing the two most important cytoskeletal structures controlling the cell division plane. By combining subcellular fractionation and microscopic in situ and in vivo co-localization methods, MPK6 was localized to the plasma membrane (PM) and the trans-Golgi network (TGN). In summary, these data suggest that MPK6 localizing to mitotic microtubules, secretory TGN vesicles and the PM is involved in cell division plane control and root development in Arabidopsis.

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Peptide Hormones and Growth Factors: Signaling Mechanisms in Endosomes☆

Laporte¹,

Posner²

2016

Reference Module in Biomedical Sciences

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Compartmentalization of the Mitogen-Activated Protein Kinase (MAPK) in Hepatic Endosomes: Association with the Internalized Epidermal Growth Factor (EGF) Receptor

Cited by 8 publications

References 43 publications

Characterization of Cdk2-Cyclin E Complexes in Plasma Membrane and Endosomes of Liver Parenchyma

Characterization of Cdk2-Cyclin E Complexes in Plasma Membrane and Endosomes of Liver Parenchyma

Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane

Peptide Hormones and Growth Factors: Signaling Mechanisms in Endosomes☆

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