Compartmentalization, Intracellular Transport, and Autophagy of Tomato Spotted Wilt Tospovirus Proteins in Infected Thrips Cells

Ullman, Diane E.; Westcot, Daphne M.; Chenault, Kelly D.; Sherwood, J. L.; German, Thomas L.; Bandla, M.; Cantone, Frank A.; Duer, H. L.

doi:10.1094/phyto-85-644

Cited by 49 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the replicating virus inside the host tissue, together with reproduction of the host, could be very costly in terms of energy and biomass conservation rates, and therefore, the number of offsprings could be reduced (Ullman et al. , ; Froissart et al. ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)

Ogada

Maiß

Poehling

2012

J Applied Entomology

View full text Add to dashboard Cite

The general principles in pathogen transmission by insects involve a complex and specific interplay, in this case between thrips, tospovirus and their shared host plant, which has led to outbreaks of crop disease epidemics of economic and social importance. The possible processes and factors driving their co‐evolution were partly studied by rearing Frankliniella occidentalis [western flower thrips (WFT)] on either tomato spotted wilt virus (TSWV)–infected or uninfected Capsicum annum leaflets throughout their larval stages. Later, pupae were transferred individually on healthy leaf discs for further studies of the influence of TSWV on WFT development and behavioural patterns. The exposure of WFT to TSWV was found to improve performance with regard to longevity and survival, with mean longevity being significantly higher in TSWV‐exposed WFT compared to unexposed ones (F(3,403) = 22.44, P < 0.0001). The observed improvement in survival was as a result of significant reduction in mortality for the WFT individuals exposed to TSWV (F(3,383) = 849.94, P < 0.0001) compared to the unexposed. However, the results showed a significant reduction in mean daily fecundity overtime (F10,10) = 246.66, P < 0.0001) and across the four treatments (F(3,30) = 6.62, P = 0.001), as well as lifetime fecundity (F(3,23) = 21.23, P < 0.0001) of the WFT exposed to TSWV compared to the unexposed reared on uninfected leaf discs. For preferential test, C. annum leaf discs infected with TSWV were more attractive to WFT as compared to healthy leaf discs (χ2(4, 34) = 112.35, P < 0.0001). These results are envisaged to contribute to a clear understanding into the plant–vector–virus interaction, which is essential for accurate diagnosis and control of the TSWV epidemic, as well as the control of F. occidentalis as crop pest.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Soon after uptake, an increase in the viral protein indicates replication of TSWV in the thrips vector (Ullman et al. , ; Moritz et al. ).…”

Section: Introductionmentioning

confidence: 99%

Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)

Ogada

Maiß

Poehling

2012

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…The virus initially infects the midgut, replicating first in the epithelium and then the muscle fibers surrounding the midgut and foregut of the developing larvae. The virus subsequently spreads via cell‐to‐cell movement to the salivary glands where it also replicates and from which it is transmitted to plants during feeding (Ullman et al., 1995, 1997; Moritz et al., 2004). The ability of a TSWV‐infected thrips to transmit the virus requires infection of the salivary glands.…”

Section: Introductionmentioning

confidence: 99%

Effects of tomato spotted wilt virus isolates, host plants, and temperature on survival, size, and development time of Frankliniella occidentalis

Stumpf¹,

Kennedy

2007

Entomologia Exp Applicata

View full text Add to dashboard Cite

Tomato spotted wilt virus (TSWV) replicates in both its plant hosts and its thrips vectors. Replication of TSWV within thrips suggests the potential for pathological effects that could affect the fitness of its vectors directly, whereas infection of the plant may alter its suitability as a host for thrips development. This study was undertaken to examine the influence of TSWV isolate, host plant, and temperature on potential direct and host‐mediated effects of virus infection of the thrips and the plant on Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), an important vector of TSWV. Neonate F. occidentalis were reared to adult eclosion on excised foliage of Datura stramonium (L.) (Solanaceae) or Emilia sonchifolia (L.) (Compositae) infected with either the CFL or RG2 isolate of TSWV, or not infected. Effects of the TSWV isolates and host plants on thrips were measured at 18.3, 23.9, and 29.4 °C. Results demonstrate significantly improved survival and a small but significant decrease in development time of F. occidentalis on TSWV‐infected plants. These effects resulted from the combined influence of the direct effects of the virus on infected thrips and plant‐mediated effects resulting from virus infection of the thrips’ host plant. Our results extend previous findings and help to explain inconsistencies among previously published reports by demonstrating that the manifestation and magnitude of effects of TSWV on F. occidentalis are dependent on host plant, virus isolate, and temperature.

show abstract

“…The first round takes place in the cells of the midgut, and the second major round takes place in the salivary glands (21,22,24,28). Ullman et al (22) suggested a ME barrier in F. occidentalis that may be blocked in the adult stage, thus explaining why adult thrips can transmit TSWV only after they have ingested the virus during their larval stage.…”

mentioning

confidence: 99%

Binding of Tomato Spotted Wilt Virus to a 94-kDa Thrips Protein

Kikkert¹,

Meurs²,

Wetering³

et al. 1998

Phytopathology®

View full text Add to dashboard Cite

Using protein blot assays, a 94-kDa thrips protein was identified that exhibited specific binding to tomato spotted wilt virus (TSWV) particles. Renaturation of the 94-kDa protein, which is conserved among the two major vector species of TSWV, Frankliniella occidentalis and Thrips tabaci, was crucial for its virus-binding properties, whereas under the same conditions no specific binding was observed with aphid (Myzus persicae) proteins. The 94-kDa protein species was present in all developmental stages of both vectoring thrips, whereas it was present mainly in the adult stage of a nonvectoring thrips species, Parthenothrips dracenae. Using antibodies against the different TSWV structural proteins, the G2 envelope glycoprotein was identified as the viral determinant involved. Because the virus-binding protein is present throughout the thrips body, but not in the gut, it may represent a receptor protein involved during circulation of the virus through its vector but probably not during viral uptake in the midgut.

show abstract

Compartmentalization, Intracellular Transport, and Autophagy of Tomato Spotted Wilt Tospovirus Proteins in Infected Thrips Cells

Cited by 49 publications

References 0 publications

Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)

Influence of tomato spotted wilt virus on performance and behaviour of western flower thrips (Frankliniella occidentalis)

Effects of tomato spotted wilt virus isolates, host plants, and temperature on survival, size, and development time of Frankliniella occidentalis

Binding of Tomato Spotted Wilt Virus to a 94-kDa Thrips Protein

Contact Info

Product

Resources

About