Compartmentalization and actin binding properties of ABP‐50: The elongation factor‐1 alpha of <i>Dictyostelium</i>

Dharmawardhane, Suranganie; Demma, Mark; Yang, Fan; Condeelis, John S.

doi:10.1002/cm.970200404

Cited by 79 publications

(63 citation statements)

References 29 publications

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“…In our study, we found that EF-1α immunofluorescent signal was co-localized with F-actin, which has been reported previously (24). We also demonstrated that the knockdown of EF-1α resulted in the decrease of growth in Du145 cells, which was consistent with the inhibition of migration and invasion.…”

Section: Discussionsupporting

confidence: 91%

Inhibition of Proliferation, Invasion, and Migration of Prostate Cancer Cells by Downregulating Elongation Factor-1α Expression

Zhu

Yan

et al. 2009

Mol Med

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Overexpression of elongation factor-1α (EF-1α) has been reported to contribute to the development and progression of various cancers. However, its role in prostate cancer (PCa) still remains poorly understood. In the present study, we investigate the influence of EF-1α in Du145, a high-grade metastatic PCa cell line, and demonstrate that EF-1α plays an essential role in cellular properties associated with tumor progression, namely cell proliferation, invasion, and migration. In this study, EF-1α expression in human PCa cell line Du145 was reduced by RNA interference (RNAi) technology, and the proliferation, invasion, and migration of EF-1α-reduced Du145 cells were examined. We also detected an EF-1α expression pattern in 20 pairs of primary PCa samples and their corresponding normal tissues. Expression of EF-1α was detectable in four PCa cell lines (22RV1, LnCap, Du145, and PC3), indicating its possible role in pathogenesis of PCa. RNAi-mediated knockdown of EF-1α expression in Du145 cells, which expressed the highest level of EF-1α among four PCa cell lines, led to a decrease in proliferation. Similarly, suppression of EF-1α inhibited Du145 cell migration and invasion through a basement membrane substitute. Furthermore, we found that the normal prostate tissues showed a relatively low level of EF-1α expression, whereas PCa tissues demonstrated significantly higher expression levels of EF-1α (P < 0.001). Taken together, these findings support the hypothesis that EF-1α affects multiple processes involved in tumor progression, and identify EF-1α as a potential therapeutic target.

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Section: Discussionsupporting

confidence: 91%

Inhibition of Proliferation, Invasion, and Migration of Prostate Cancer Cells by Downregulating Elongation Factor-1α Expression

Zhu

Yan

et al. 2009

Mol Med

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“…Immunoprecipitates were washed and processed for SDS-PAGE and Western blotting as described in Ref. 40. Immunoprecipitates of eIF4G antibody or control monoclonal antibody, supernatants, and total lysates were immunoblotted with anti-eIF4G (top half of gel) or anti-eIF4E (bottom half of same gel) to visualize eIF4G as a 220-kDa band and eIF4E as a 25-kDa band.…”

Section: Methodsmentioning

confidence: 99%

The Soy Isoflavone Equol May Increase Cancer Malignancy via Up-regulation of Eukaryotic Protein Synthesis Initiation Factor eIF4G

Parra

Otero-Franqui

Martínez-Montemayor

et al. 2012

Journal of Biological Chemistry

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Background: The molecular mechanisms of soy isoflavones in metastatic cancer remain to be elucidated. Results: Equol, a daidzein metabolite, regulates eIF4G-mediated cap-independent protein synthesis initiation of proteins relevant for cancer malignancy. Conclusion: Equol is a potent regulator of the cancer promoting effects of dietary daidzein. Significance: Consumption of soy may not be advisable for patients with aggressive breast cancer.

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“…; Dharmawardhane et al, 1991). The use of this antiserum for the immunoblot shown in Figure 5A indicates the identity of pp50, which eluted from both the tubulin (lane 1) and CaM (lane 2) affinity matrices.…”

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confidence: 89%

A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.

1994

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The microtubules (MTs) of higher plant cells a= organized into arrays with essential functions in plant cell growth and differentiation; however, molecular mechanisms underlying the organization and regulation of these arrays remain largely unknown. We have approached this problem using tubulin affinity chromatography to isolate carrot proteins that interact with MTs. From these proteins, a 50-kD polypeptide was selectively purified by exploiting its Ca2+-dependent binding to calmodulin (CaM). This polypeptide was identified as a homolog of elongation factor-lu (EF-1a)-a highly conserved and ubiqultous protein translation factor. The carrot EF-la homolog bundles MTs in vitro, and moreover, this bundling is modulated by the addition of Ca2+ and CaM together (Ca2+/CaM). A direct binding between the EF-la homolog and MTs was demonstrated, providing nove1 evidence for such an interaction. Based on these findings, and others discussed herein, we propose that an EF-la homolog mediates the lateral association of MTs in plant celk by a Ca2+/CaM-sensitive mechanism. INTRODUCTIONMicrotubules (MTs) participate in a number of essential processes in eukaryotes (MacRae, 1992b). In cells of higher plants, MTs are generally arranged into four structurally and functionally distinct, cell cycle-dependent MT arrays (Goddard et al., 1994;Lambert and Lloyd, 1994). During interphase, the MT array in a cell's cortex is involved in the extracellular deposition of cellulose microfibrils (Giddings and Staehelin, 1991). During G2 of the cell cycle, this array is succeeded by a narrower preprophase band involved in establishing the site of the incipient division plane (Palevitz, 1991; Wick, 1991). The preprophase band resolves into the mitotic spindle apparatus as M phase progresses Palevitz, 1993). The fourth MT array, the phragmoplast, appears as cytokinesis begins and is involved in depositing the new cell plate at the position previously marked by the preprophase band (Lloyd, The bases by which plant MTs are organized into higher order arrays and by which those arrays are regulated remain largely unknown (Lambert, 1993). MTs are hollow cylinders with 25-nm diameters, and tubulin is the principal protein subunit of MTs. MTs assembled from pure tubulin have a limited range of behavior-they assemble or disassemble. This limited range of behavior can only partly account for the diverse structures and functions of MT arrays.Nearly all species have multiple tubulin genes encoding various tubulin isotypes (for plant genes, see Goddard et al., 1994), and it has been proposed that the isotypes may provide a 1991).To whom correspondence should be addressed. means for serving different MT functions (Fulton and Simpson, 1976). However, nearly all existing evidence indicates that tubulin isotypes are functionally interchangeable (LudueAa, 1993). In general, tubulins are COnseNed even between kingdoms (Fosket and Morejohn, 1992; Burns and Surridge, 1994). In fact, animal brain tubulin that is microinjected into living plant cells incorporates into t...

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Compartmentalization and actin binding properties of ABP‐50: The elongation factor‐1 alpha of Dictyostelium

Cited by 79 publications

References 29 publications

Inhibition of Proliferation, Invasion, and Migration of Prostate Cancer Cells by Downregulating Elongation Factor-1α Expression

Inhibition of Proliferation, Invasion, and Migration of Prostate Cancer Cells by Downregulating Elongation Factor-1α Expression

The Soy Isoflavone Equol May Increase Cancer Malignancy via Up-regulation of Eukaryotic Protein Synthesis Initiation Factor eIF4G

A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.

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