Compartive growth of Candida lipolytica On Glucose and n-Hexadecane

Nyns, Edmond‐Jacques; Auquière, J P; Chiang, Ni-Na; Wiaux, A. L.

doi:10.1038/215177a0

Cited by 20 publications

(3 citation statements)

References 3 publications

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“…Y. lipolytica can use n ‐alkanes and 1‐alkenes as carbon sources [18–21]. Polymethylated and chlorinated alkanes are also assimilated by this fungus [22, 23].…”

Section: Physiologymentioning

confidence: 99%

Physiology and genetics of the dimorphic fungusYarrowia lipolytica

Barth¹,

Gaillardin²

1997

FEMS Microbiol Rev

436

100

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The ascomycetous yeast Yarrowia lipolytica (formerly Candida, Endomycopsis, or Saccharomyces lipolytica) is one of the more intensively studied 'non-conventional' yeast species. This yeast is quite different from the well-studied yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe with respect to its phylogenetic evolution, physiology, genetics, and molecular biology. However, Y. lipolytica is not only of interest for fundamental research, but also for biotechnological applications. It secretes several metabolites in large amounts (i.e. organic acids, extracellular proteins) and the tools are available for overproduction and secretion of foreign proteins. This review presents a comprehensive overview on the available data on physiology, cell biology, molecular biology and genetics of Y. lipolytica.

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“…Y. lipolytica can use n ‐alkanes and 1‐alkenes as carbon sources [18–21]. Polymethylated and chlorinated alkanes are also assimilated by this fungus [22, 23].…”

Section: Physiologymentioning

confidence: 99%

Physiology and genetics of the dimorphic fungusYarrowia lipolytica

Barth¹,

Gaillardin²

1997

FEMS Microbiol Rev

436

100

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“…Yarrowia lipolytica is able to utilize a various range of carbon sources, such as glucose, glycerol, alcohols, acetate, and a high range of hydrophobic substrates such as plant oils, fats, fatty acids, and n ‐alkanes (Ishikura & Foster, ; Nyns et al ., ; Klug & Markovetz, ; Barth & Gaillardin, ). A review of hydrophobic substrate utilization in Y. lipolytica – including the related pathways, enzymes, and corresponding genes – is given by Fickers et al .…”

Section: Introductionmentioning

confidence: 99%

A newly identified fatty alcohol oxidase gene is mainly responsible for the oxidation of long-chain ω-hydroxy fatty acids inYarrowia lipolytica

et al. 2014

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Nine potential (fatty) alcohol dehydrogenase genes and one alcohol oxidase gene were identified in Yarrowia lipolytica by comparative sequence analysis. All relevant genes were deleted in Y. lipolytica H222ΔP which is lacking β-oxidation. Resulting transformants were tested for their ability to accumulate ω-hydroxy fatty acids and dicarboxylic acids in the culture medium. The deletion of eight alcohol dehydrogenase genes (FADH, ADH1-7), which may be involved in ω-oxidation, led only to a slightly increased accumulation of ω-hydroxy fatty acids, whereas the deletion of the fatty alcohol oxidase gene (FAO1), which has not been described yet in Y. lipolytica, exhibited a considerably higher effect. The combined deletion of the eight (fatty) alcohol dehydrogenase genes and the alcohol oxidase gene further reduced the formation of dicarboxylic acids. These results indicate that both (fatty) alcohol dehydrogenases and an alcohol oxidase are involved in ω-oxidation of long-chain fatty acids whereby latter plays the major role. This insight marks the first step toward the biotechnological production of long-chain ω-hydroxy fatty acids with the help of the nonconventional yeast Y. lipolytica. The overexpression of FAO1 can be further used to improve existing strains for the production of dicarboxylic acids.

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“…lipolytica [ 151 was obtained as the strain 599 from the Centraalbureau voor Schimmelcultures (Baarn, The Netherlands). Cyanide-sensitive cells were grown in a synthetic medium (Yeast Nitrogen Base, Difco), supplemented with 1 % glucose, and harvested in the exponential phase of growth as described earlier [16].…”

Section: Culture Of Cellsmentioning

confidence: 99%

Kinetics of the Respiration of Cyanide‐Insensitive Mitochondria from the Yeast Saccharomycopsis lipolytica

Troostembergh

Nyns

1978

European Journal of Biochemistry

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1. In cyanide-insensitive mitochondria from Succhuromycop.yis lipolytica, the respiration can proceed either via the cytochrome c oxidase or via an alternative oxidase. This alternative, mitochondrial, cyanide-insensitive respiration can be integrated in the respiratory kinetic model of the mobile ubiquinone pool, originally designed by Kroger and Klingenberg.2. In the proposed kinetic model, three groups of electron carriers, a donor, catalyzing the electron transfer from the oxidation substrate to ubiquinone, and two acceptors, catalyzing the electron transfer from ubiquinol to oxygen, via either the cytochrome c oxidase or the alternative oxidase, are linked together by a mobile ubiquinone pool. The donor and acceptors are characterized by their respective activities. It is assumed, in the simplest way, that ubiquinol gives off its reducing equivalents randomly to either cytochrome or alternative acceptors. The kinetic model is expressed mathematically by a set of three equations relating the respiration rates to the activities.3. The proposed model, an alternative to Bahr and Bonner's kinetic model, does not necessarily require a modulation factor, that is, a variable portion of the alternative acceptors being, at times, inactive.4. As three respiration rates can be experimentally measured in a independent way, the set of three equations can be solved unambiguously for the donor and acceptor activities.5. The proposed model was experimentally assessed by kinetic analysis, after modifying the cytochrome acceptor activity by either ADP, antimycin or added cytochrome c and/or the alternative acceptor activity by either benzhydroxamic acid or submission of the mitochondria to an osmotic shock.6. The kinetic data fitted the proposed model and hence, more generally, provide further evidence in favor of the respiratory model of the mobile ubiquinone pool and also in favor of the branching point of the alternative respiratory pathway on the main respiratory pathway at the level of ubiquinone.7. That the donor and acceptor activities corresponded to their definition in the Kroger and Klingenberg model was strengthened by the observation that the stimulatory or inhibitory agents only affected the acceptor activity characterizing the group of electron carriers where the site of action of these agents is known to be located; also by the demonstration that the ratios of donor to cytochrome acceptor activities derived from the proposed model, corresponded to the same ratios computed independently from antimycin titration data ; and furthermore by the fact that the cytochrome acceptor activity varied linearly with the number of inhibited sites for antimycin. 8. The model also explains why the total respiration rate is always inferior to the mathematical sum of the respiration rates via the cytochrome c oxidase, and via the alternative oxidase, measured separately, and why upon titration by hydroxamic acids, a linear relation exists between the total respiration rate and the respiration rates via the alternative oxidase, bo...

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Compartive growth of Candida lipolytica On Glucose and n-Hexadecane

Cited by 20 publications

References 3 publications

Physiology and genetics of the dimorphic fungusYarrowia lipolytica

Physiology and genetics of the dimorphic fungusYarrowia lipolytica

A newly identified fatty alcohol oxidase gene is mainly responsible for the oxidation of long-chain ω-hydroxy fatty acids inYarrowia lipolytica

Kinetics of the Respiration of Cyanide‐Insensitive Mitochondria from the Yeast Saccharomycopsis lipolytica

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