Comparisons of the results of peripheral nerve defect repair with fibrin conduit and autologous nerve graft: An experimental study in rats

Longo, Marco Vinicius Losso; Faria, P.h.d. José Carlos Marques de; Isaac, César; Nepomuceno, André Coelho; Teixeira, Nuberto Hopfgartner; Gemperli, Rolf

doi:10.1002/micr.22413

Cited by 21 publications

(12 citation statements)

References 41 publications

(50 reference statements)

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“…Clinically, if the nerve ends cannot be approximated without tension, bridging materials are necessary for reconstruction. Many recent studies have reported acceptable results using nerve conduits in peripheral nerve repair (Arnaout, Fontaine, & Chantelot, ; Cirillo et al, ; Klein, Vykoukal, Felthaus, Dienstknecht, & Prantl, ; Longo et al, ; Schmauss, Finck, Liodaki, et al, ). However, these outcomes are not satisfactory and various improvements to these methods have been investigated (Eren, Öksüz, Küçükodaci, et al, ).…”

Section: Introductionmentioning

confidence: 99%

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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Introduction A Bio 3D printed nerve conduit was reported to promote nerve regeneration in a 5 mm nerve gap model. The purpose of this study was to fabricate Bio 3D nerve conduits suitable for a 10 mm nerve gap and to evaluate their capacity for nerve regeneration in a rat sciatic nerve defect model. Materials and Methods Eighteen F344 rats with immune deficiency (9–10 weeks old; weight, 200–250 g) were divided into three groups: a Bio 3D nerve conduit group (Bio 3D, n = 6), a nerve graft group (NG, n = 6), and a silicon tube group (ST, n = 6). A 12‐mm Bio 3D nerve conduit or silicon tube was transplanted into the 10‐mm defect of the right sciatic nerve. In the nerve graft group, reverse autografting was performed with an excised 10‐mm nerve segment. Assessments were performed at 8 weeks after the surgery. Results In the region distal to the suture site, the number of myelinated axons in the Bio 3D group were significantly larger compared with the silicon group (2,548 vs. 950, p < .05). The myelinated axon diameter (MAD) and the myelin thickness (MT) of the regenerated axons in the Bio 3D group were significantly larger compared with those of the ST group (MAD: 3.09 vs. 2.36 μm; p < .01; MT: 0.59 vs. 0.40 μm, p < .01). Conclusions This study indicates that a Bio 3D nerve conduit can enhance peripheral nerve regeneration even in a 10 mm nerve defect model.

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Section: Introductionmentioning

confidence: 99%

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

et al. 2019

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“…This use of FG as an adjunct to conventional neuro-suturing resulted in better fiber alignment and axonal regeneration [31]. FG has also been used as a conduit material [32–36]. In addition, FG has been used as a filler within the lumen of non-porous conduits [37, 38], and as scaffolds to deliver growth factors for nerve regeneration [39, 40].…”

Section: Introductionmentioning

confidence: 99%

Fibrin glue as a stabilization strategy in peripheral nerve repair when using porous nerve guidance conduits

Bhatnagar

Bushman

Murthy

et al. 2017

J Mater Sci: Mater Med

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Porous conduits provide a protected pathway for nerve regeneration, while still allowing exchange of nutrients and wastes. However, pore sizes >30 µm may permit fibrous tissue infiltration into the conduit, which may impede axonal regeneration. Coating the conduit with Fibrin Glue (FG) is one option for controlling the conduit’s porosity. FG is extensively used in clinical peripheral nerve repair, as a tissue sealant, filler and drug-delivery matrix. Here, we compared the performance of FG to an alternative, hyaluronic acid (HA) as a coating for porous conduits, using uncoated porous conduits and reverse autografts as control groups. The uncoated conduit walls had pores with a diameter of 60 to 70 µm that were uniformly covered by either FG or HA coatings. In vitro, FG coatings degraded twice as fast as HA coatings. In vivo studies in a 1 cm rat sciatic nerve model showed FG coating resulted in poor axonal density (993 ± 854 #/mm2), negligible fascicular area (0.03 ± 0.04 mm2), minimal percent wet muscle mass recovery (16 ± 1 in gastrocnemius and 15 ± 5 in tibialis anterior) and G-ratio (0.73 ± 0.01). Histology of FG-coated conduits showed excessive fibrous tissue infiltration inside the lumen, and fibrin capsule formation around the conduit. Although FG has been shown to promote nerve regeneration in non-porous conduits, we found that as a coating for porous conduits in vivo, FG encourages scar tissue infiltration that impedes nerve regeneration. This is a significant finding considering the widespread use of FG in peripheral nerve repair.Graphical Abstract

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“…Autograft transplantation is a gold standard for long‐range nerve defects . Nevertheless, its shortcomings are obvious, including shortages in autografts, lesions at donor site, and high cost for multiple surgeries . Nerve guidance channels (NGCs) are invented for large nerve gaps as an alternative implant from tissue engineering .…”

Section: Introductionmentioning

confidence: 99%

3D Manufacture of Gold Nanocomposite Channels Facilitates Neural Differentiation and Regeneration

Qian

Song

Zheng

et al. 2018

Adv Funct Materials

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Conductive nerve guidance channels are promising alternative therapies in peripheral nerve tissue engineering because they have excellent biocompatibility, biodegradation, and electrical conductivity. Gold, a kind of conductive material, is investigated widely concerning its potential roles in promoting peripheral nerve repair. In the present study, a polydopamine-coated gold/ polycaprolactone nanoscaffold is fabricated via a multilayer molding method and its proliferative, adhesive, and neural differentiation potential for bone marrow mesenchymal stem cells (BMSCs) and Schwann cells (SCs) in vitro is evaluated. Functional, electrophysiological evaluation, and morphological assessment all exhibit satisfactory recovery of sciatic nerves with increased thickness and number of myelinated fibers in vivo. In addition, increased microvessels are confirmed in gold nanocomposite channels, indicating their potential benefits in angiogenesis. Functional regeneration is further enhanced by neurotrophic growth factors released from BMSC and SC loading. The gold nanocomposite channel will have great potential in peripheral nerve restoration.

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Comparisons of the results of peripheral nerve defect repair with fibrin conduit and autologous nerve graft: An experimental study in rats

Cited by 21 publications

References 41 publications

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

A scaffold‐free Bio 3D nerve conduit for repair of a 10‐mm peripheral nerve defect in the rats

Fibrin glue as a stabilization strategy in peripheral nerve repair when using porous nerve guidance conduits

3D Manufacture of Gold Nanocomposite Channels Facilitates Neural Differentiation and Regeneration

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