Comparisons of Surface Markers on Herpesvirus-associated Lymphoid Cells of Nonhuman Primates and Established Human Lymphoid Cell Lines

Rabin, Harvey; Wallen, William C.; Neubauer, R H; Epstein, M. A.

doi:10.1159/000397554

Cited by 5 publications

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“…The virus can induce acute lymphomas or lymphoid leukemias in several species of New World monkeys (21). Herpesvirus saimiritransformed cells are T lymphocytes of the CD-8 class (14,23,25,26).…”

mentioning

confidence: 99%

Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits

Medveczky

Szomolányi

Hesselton

et al. 1989

J Virol

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Herpesvirus saimiri is a primate tumor virus that induces acute T-cell lymphomas in New World monkeys. Strains of this virus have been previously classified into three groups on the basis of extreme DNA variability of the rightmost region of unique L-DNA. To compare the oncogenic potentials of various strains, we inoculated New Zealand White rabbits with viruses representing groups A, B, and C of herpesvirus saimiri. The results showed that a group C strain were highly oncogenic in New Zealand White rabbits; however, group A or B viruses were not oncogenic in these rabbits. Analysis of DNAs of tumor tissues and lymphoid cell lines established from tumors showed that the viral genome exists in circular episomal form. To identify which part of the genome of the group C strain is responsible for the highly oncogenic phenotype, group B-C recombinant strains were constructed by an efficient drug selection technique. Two group B recombinant strains in which the right-end 9.2 kilobase pairs of unique DNA is replaced by group C virus DNA were oncogenic in rabbits, indicating that the rightmost sequences contribute to the oncogenic properties of the group C strain. Oncogenicity of herpesvirus saimiri has been traditionally evaluated in New World monkeys; infection of rabbits with group C strain 484-77 offers a much more accessible animal model to study the mechanism of oncogenicity of this virus.

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mentioning

confidence: 99%

Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits

Medveczky

Szomolányi

Hesselton

et al. 1989

J Virol

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“…In the first series of experiments, two rabbits were each immunized with a globulin fraction prepared by ammonium sulphate precipitation from the culture fluid in which the marmoset T cell line 70-N2 had been grown. It is important to note that this line has been shown to form spontaneous rosettes with sheep erythrocytes (E-rosettes), to lack receptors for complement, to lack classical immunoglobulin isotypes, and to express T cell-type surface glycoproteins Rabin et al, 1975;Marchalonis et al, 1980;Neubauer et al, 1981;Strnad et al, 1979). The antisera produced were tested against several populations of isolated human lymphocytes including E positive T cells, E negative cells including Ig positive B lymphocytes as well as null cells and a series of monoclonal B cell, T cell and null cell lines.…”

Section: R E S U L T Smentioning

confidence: 99%

A Marmoset T Lymphoma Which Functions as a Human Amplifier T Cell

Marchalonis

Strelkauskas

1981

Int J Immunogenetics

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SUMMARY A long‐term in vitro grown T cell line derived from a cotton‐topped marmoset (Saguinus oedipus)) infected with Herpesvirus saimiri was found to share surface antigens with human amplifier T cells and to augment the capacity of human B cells to secrete immunoglobulin. This is the first demonstration of T/B collaboration across such a large phylogenetic barrier and might have interesting implications for understanding the nature of molecular interactions mediating cell/cell cooperation.

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“…The rapid develop ment of the disease suggests that H. saimiri simultaneously transforms several cells and causes a polyclonal acute lymphoproliféra tion. Both in vivo and in vitro transformed cells are T lymphocytes of the CD-8 class [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Paradoxically, H. saimirí can infect many cell types such as fibroblasts and kidney cells, but transformed cells are uniformly T cells [4][5][6]. Purified viral DNA, viral DNA fragments, or tumor cell DNA cannot trans form NIH 3T3 cells [Szomolanyi and Mul der, personal commun.]…”

Section: Introductionmentioning

confidence: 99%

Expression of Interleukin 2 Receptors in T Cells Transformed by Strains of <i>Herpesvirus saimiri</i> Representing Three DNA Subgroups

Medveczky¹,

Medveczky²

1989

Intervirology

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Eight T lymphoblastoid cell lines transformed by Herpesvirus saimiri were studied for requirement of interleukin 2 (IL-2) for growth in tissue culture and expression of the IL-2 receptor. The results show that four cell lines grew independent of IL-2, but four other cell lines required IL-2 for optimal growth. However, all H. saimiri transformed cells expressed IL-2 receptors, regardless of IL-2 requirement. Between 2,250 and 8,800 high-affinity receptor sites/cell were detected by ligand-binding assays; in contrast, only about 1,000 sites/cell were in an uninfected IL-2-dependent marmoset T cell line. Surface-bound IL-2 was internalized and antibodies to the receptor inhibited cell growth. Expression of IL-2 receptors may allow expansion of tumor cells in vivo in response to endogenous or exogenous IL-2.

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Comparisons of Surface Markers on Herpesvirus-associated Lymphoid Cells of Nonhuman Primates and Established Human Lymphoid Cell Lines

Cited by 5 publications

References 0 publications

Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits

Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits

A Marmoset T Lymphoma Which Functions as a Human Amplifier T Cell

Expression of Interleukin 2 Receptors in T Cells Transformed by Strains of <i>Herpesvirus saimiri</i> Representing Three DNA Subgroups

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