Comparisons of Salinity Adaptation in Terms of Growth, Body Composition, and Energy Budget in Juveniles of Rainbow and Steelhead Trouts (Oncorhynchus mykiss)

Xiong, Yinghuai; Wang, Xin; Dong, Shuanglin; Wang, Fang; Yang, Jingwen; Zhou, Ye

doi:10.1007/s11802-019-3770-4

Cited by 11 publications

(5 citation statements)

References 44 publications

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Section: Discussionmentioning

confidence: 99%

“…Therefore, not only osmotic pressure regulation, but also other processes such as lipid metabolism and the composition of fatty acids in tissues could be affected [7]. In rainbow trout, both osmo-regulation and energy metabolism were shown to be the core regulatory contents affected by salinity change [8]. In order to explore the effect of salinity acclimation on bone metabolism of rainbow trout, the global miRNA expression profiles in ontogenic scales were examined, and the DETGs were associated with terms such as metal ion binding, ATP binding, nucleotide binding and transferase activity; KEGG pathway analysis also revealed that they were mainly enriched in bone metabolism-related signaling pathways such as MAPK, calcium and Wnt [4].…”

Section: Discussionmentioning

confidence: 99%

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Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation

Yang

Zhou

et al. 2022

Animals

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In order to explore the potential effects of salinity acclimation on bone metabolism of rainbow trout (Oncorhynchus mykiss), transcriptional information of regenerated scales under salinity acclimation (sea water, SW) was compared to those of fish under fresh water (FW) environments. According to the high-throughput sequencing results, a total of 2620 significantly differentially expressed genes (DEGs) were identified in the data of SW vs FW. Compared with the FW group, six significantly downregulated and 44 significantly upregulated miRNAs were identified in the SW scales (p < 0.05). Furthermore, a total of 994 significantly differentially expressed target genes (DETGs) were identified from the 50 significantly differentially expressed miRNAs (DE miRNAs). Gene ontology analysis of the aforementioned DETGs was similar to the results of the differentially expressed genes (DEGs) obtained from mRNA-seq data, these genes were mainly related to ion metabolism. KEGG enrichment analysis of the DEGs and DETGs suggested that many significantly enriched pathways were related to the energy metabolism pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation

Yang

Zhou

et al. 2022

Animals

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“…As reported, this species can develop and grow normally in seawater [2]. This advantage has long been focused since the adaptation to salinity is closely related to the development, quality and timing of transfer of the sh into seawater for aquaculture [3,4]. Despite the efforts to understand how O. mykiss cope with different salinities (osmoregulation mechanism), not much consideration has been given to how the other systems (such as skeletal system) might respond to the salinity adaptation.…”

Section: Introductionmentioning

confidence: 99%

Effects of Salinity Acclimation on Histological Characteristics and miRNA Expression Profiles of Scales in Juvenile Rainbow Trout (Oncorhynchus Mykiss)

Zhou

Wang

Zhao

et al. 2022

Preprint

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Background: The scales serve as an ideal model for studying the regulatory mechanism of bone homeostasis in fish. To explore the effect of salinity acclimation on bone metabolism of juvenile rainbow trout (Oncorhynchus mykiss), three sampling time points during salinity acclimation (7D, 14D and 21D) were selected to detect variations in histological characteristics. In the histological analysis, osteoblast marker enzymes alkaline phosphatase (ALP), osteoclast marker tartrate-resistant acid phosphatase (TRAcP) and calcium salt deposit areas (Von Kossa′s) were detected. Changes in calcium (Ca), phosphorus (P) and the molar mass ratio of calcium to phosphorus (Ca/P) in the scales were also detected by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, the global MicroRNA (miRNA) expression profiles during salinity acclimation were examined using Illumina sequencing platform because of their important regulatory roles in teleost biological processes.Results: Twelve independent miRNA libraries were constructed, a total of 664 known and 92 putative novel miRNAs were identified. A total of 290 DEC miRNAs (differentially expressed miRNAs from clusters with significant trends in the cluster analysis) with five types of clustering patterns were obtained, and 5,957 DEC mRNAs (differentially expressed target genes of 22,370 DEC miRNA showed a significant trend in cluster analysis) with six types of clustering patterns were screened according to transcriptome sequencing data. To better understand the functions of the DE miRNAs, 5,957 DEC mRNAs were used for GO and KEGG analysis, and they were significantly enriched in bone metabolism related signaling pathways such as MAPK signaling pathway, Calcium signaling pathway, Wnt signaling pathway, Mineral absorption and NF-kappa B signaling pathway. Six DE miRNAs were randomly selected and their expression were verified by quantitative real-time PCR (qRT-PCR), the expression trends were consistent with the results of transcriptome sequencing.Conclusions: The DEC miRNAs identified in this study might play an important role in regulation of bone metabolism during salinity acclimation, relative genes or pathways could serve as key candidates for further studies to elucidate molecular mechanism of teleost bone metabolism, and help performing salinity acclimation and developing marine culture of salmonid species.

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“…The strong ability of salinity adaptation makes it possible for these species to be cultured in diving cages in the open sea [ 3 , 4 ]. The advantage of salinity acclimation has long been focused since it is closely related to the development, quality and timing of transfer of the fish into seawater for aquaculture [ 5 , 6 ]. Despite the efforts to understand how O. mykiss cope with different salinities (osmoregulation mechanism), not much consideration has been given to how the other systems (such as skeletal system) might respond to the salinity adaptation.…”

Section: Introductionmentioning

confidence: 99%

Effects of salinity acclimation on histological characteristics and miRNA expression profiles of scales in juvenile rainbow trout (Oncorhynchus mykiss)

et al. 2022

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Background The scales serve as an ideal model for studying the regulatory mechanism of bone homeostasis in fish. To explore the effect of salinity acclimation on bone metabolism of juvenile rainbow trout (Oncorhynchus mykiss), three sampling time points during salinity acclimation (7D, 14D and 21D) were selected to detect variations in histological characteristics. In the histological analysis, osteoblast marker enzymes alkaline phosphatase (ALP), osteoclast marker tartrate-resistant acid phosphatase (TRAcP) and calcium salt deposit areas (Von Kossa′s) were detected. Changes in calcium (Ca), phosphorus (P) and the molar mass ratio of calcium to phosphorus (Ca/P) in the scales were also detected by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, the global MicroRNA (miRNA) expression profiles during salinity acclimation were examined using Illumina sequencing platform because of their important regulatory roles in teleost biological processes. Results Twelve independent miRNA libraries were constructed, a total of 664 known and 92 putative novel miRNAs were identified. A total of 290 differentially expressed (DE) miRNAs were found in clusters with significant trends in the cluster analysis, and five types of clustering patterns were obtained; 22,374 DE predicted target genes of the aforementioned 290 DE miRNAs were obtained, 5957 of which clustered in six types of clustering patterns with a significant trend. To better understand the functions of the DE miRNAs, GO and KEGG analysis was performed on the 5957 target genes, as a result, they were significantly enriched in bone metabolism related signaling pathways such as MAPK signaling pathway, Calcium signaling pathway, Wnt signaling pathway, Mineral absorption and NF-kappa B signaling pathway. Six DE miRNAs were randomly selected and their expression were verified by quantitative real-time PCR (qRT-PCR), the expression trends were consistent with the results of transcriptome sequencing. Conclusions The DE miRNAs and DE target genes identified in this study might play an important role in regulation of bone metabolism during salinity acclimation, relative genes or pathways could serve as key candidates for further studies to elucidate molecular mechanism of teleost bone metabolism, and help performing salinity acclimation and developing marine culture of salmonid species.

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Comparisons of Salinity Adaptation in Terms of Growth, Body Composition, and Energy Budget in Juveniles of Rainbow and Steelhead Trouts (Oncorhynchus mykiss)

Cited by 11 publications

References 44 publications

Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation

Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation

Effects of Salinity Acclimation on Histological Characteristics and miRNA Expression Profiles of Scales in Juvenile Rainbow Trout (Oncorhynchus Mykiss)

Effects of salinity acclimation on histological characteristics and miRNA expression profiles of scales in juvenile rainbow trout (Oncorhynchus mykiss)

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