Comparisons of growth of Eucalyptus camaldulensis from seeds and tissue culture: root, shoot and leaf morphology of 9-month-old plants grown in deep sand and sand over clay

Bell, David T.; Moezel, Paul G. van der; Bennett, I.J.; McComb, J.; Wilkins, Carol F.; Marshall, Simeon C.B.; Morgan, Anne L.

doi:10.1016/0378-1127(93)90167-l

Cited by 24 publications

(16 citation statements)

References 10 publications

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“…Variation in plant height among individuals of each clone was considerably less than was shoot biomass production. Coefficients of variation, ranging from 5% to 8%, were similar to the mean variation (8%) in plant height reported for E. camaldulensis replicate clones from different provenances (Bell et al, 1993).…”

supporting

confidence: 79%

Screening Eucalyptus Clones for Salt Tolerance

Grieve¹,

Guzy²,

Poss³

et al. 1999

HortSci

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Agroforestry plantations offer environmentally acceptable strategies for the reuse of saline drainage waters. Tree species suitable for use in such systems must be selected for survival and sustained growth under highly saline conditions. In this screening trial, four clones of Eucalyptus camaldulensis Dehn. (4543, 4544, 4573, and 4590) and one clone of E. rudis Endl. (4501) were grown in greenhouse sand cultures irrigated with sodium sulfate-dominated waters. Solution compositions were prepared to simulate saline drainage waters typically found in the San Joaquin Valley of California. Electrical conductivities of the solutions ranged from 2 to 28 dS·m -1 . Treatments were replicated three times. All plants survived and were harvested after 7 weeks under saline treatment. Plant height was measured weekly and shoot biomass was determined at final harvest. The salinity levels that resulted in a 50% reduction in biomass production (C 50 ) were 16.4 (4573), 17.1 (4543), 17.7 (4544), 29.0 (4590), and 30.0 dS·m -1 (4501). Over the range of salinities from 4 to 20 dS·m -1 , clones 4501, 4590, and 4573 generally maintained higher relative growth rates (RGR) than did clones 4544 and 4543. However, at the highest salinity, RGRs of clones 4501, 4544, and 4573 were significantly greater than those of clones 4543 and 4590. Assessed on the basis of biomass production, clones 4501 (E. rudis) and 4590 (E. camaldulensis) showed exceptional potential for use in agroforestry systems where the saline drainage waters are sodium sulfate-dominated.

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confidence: 79%

Screening Eucalyptus Clones for Salt Tolerance

Grieve¹,

Guzy²,

Poss³

et al. 1999

HortSci

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“…Further, with respect to rooting, the emphasis has been on root induction and percent rooting, rather than on root quality prior to and post acclimatisation. An exception is the work by Bell et al (1993) which revealed no growth morphological differences between the roots of E. camaldulensis obtained from seed and tissue cultured plants after 9 months of field growth. In addition, both plant types exhibited sinker roots that were equally capable of penetrating heavy clay soils, although one clonal line displayed root architecture that was found predominantly in the upper 20 cm of the soil profile.…”

Section: Introductionmentioning

confidence: 80%

Auxin stability and accumulation during in vitro shoot morphogenesis influences subsequent root induction and development in Eucalyptus grandis

2011

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Recent results showed that after 16 months in the field, micropropagated eucalyptus plants have an inferior root system to cuttings. Such differences may be due to the plant growth regulators supplied during the culture stages of standard protocols, which are targeted at optimising plantlet yields and not root quality. This study investigated such a proposal, focusing on auxins in an easyto-root clone. Initial results showed that the auxin provided in the standard protocol (NAA for multiplication and IBA for elongation) enabled 100% rooting in auxin-free medium, where rooting was faster than on IBA-rooting media. When auxin supply was omitted from multiplication and restricted to NAA or IAA during elongation, rooting in an auxin-free medium was reduced to 68 and 31%, respectively, reflecting the stabilities of these auxins in plant tissues. Additionally, 15% of shoots from the NAA-medium and 65% from the IAA-medium produced roots with altered graviperception. GC-MS analysis of these shoots revealed a relationship between free IAA-availability and altered graviperception. This was further tested by adding the IAA-specific transport inhibitor 2,3,5-triiodobenzoic acid to rooting media with IBA, IAA or NAA, which resulted in 100, 70.9 and 20.6% rooting, respectively. At least 40% of the sampled root tips had atypical starch grain deposition and abnormal graviperception. It is proposed that, at least in this clone, while IBA and NAA can be used for in vitro root induction, IAA is necessary for development of graviresponse.

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“…Establishment of plant varies species to species. Bell et al ( 1993 ) studied the behavior of micropropagated plants and seedling. They found some variation in the initial phase of growth, but soon those variations were lost after 40 days.…”

Section: Rooting Hardening and Field Trialsmentioning

confidence: 99%

Micropropagation of Plants

Singh

2015

Plant Biology and Biotechnology

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Micropropagation is a rapid multiplication of a selected plant using in vitro culture techniques. In this chapter various aspects of micropropagation have been discussed. The propagation of selected plant through micropropagation would be useful for raising plantation using apical and nodal segment. They are best for micropropagation and mostly result in true to type plants. These segments upon the subsequent subcultures result in a number of multiple shoots. These multiple shoots on elongation allowed to root in vitro. After rooting, they are in vitro hardened and transferred to fi eld. The potential of plant tissue culture is well recognized, as it increases agricultural production and generates rural employment. But the high cost of production on micropropagation is a major bottleneck. Low-cost protocol development can popularize this method. High multiplication rate, use of low-cost chemicals, high rooting and survival percentage, and use of ex vitro rooting method can minimize the expense of any protocol. Presently, both the developing and the developed countries require lowcost technologies to progressively reduce the cost of production. The performance of tissue plants generally better than cutting and seedling. Initially, some morphological changes occur in micropropagated plant, but in the course of time, they minimize. Somaclonal variation caused during callus cultures can be used to generate variants for hybridization. Variation in phenotype of plants produced in tissue culture can be characterized as either temporary or permanent. Temporary variation includes increased branching, greater susceptibility to disease, and lack of uniform response. Micropropagated plants need to be monitored to get long-term data for its

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Comparisons of growth of Eucalyptus camaldulensis from seeds and tissue culture: root, shoot and leaf morphology of 9-month-old plants grown in deep sand and sand over clay

Cited by 24 publications

References 10 publications

Screening Eucalyptus Clones for Salt Tolerance

Screening Eucalyptus Clones for Salt Tolerance

Auxin stability and accumulation during in vitro shoot morphogenesis influences subsequent root induction and development in Eucalyptus grandis

Micropropagation of Plants

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