Comparisons of ambient spray ionization imaging methods

Hamid, Tanam S.; Lostun, Dragos; Cabral, Elaine C.; Garrett, Rafael; Böhme, Diethard K.; Ifa, Demian R.

doi:10.1016/j.ijms.2014.07.035

Cited by 11 publications

(15 citation statements)

References 27 publications

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“…The distribution of major lipids in mouse and rat brain tissue sections is well established, making rodent brain tissue sections ideal samples for developing novel liquid extraction techniques and other technical advancements in MSI. 22,30,36,41,60,73,104,105,107,125 Lipid distributions in brain tissue sections using both DESI MSI and EASI MSI have been reported in two related studies. 22,30 Both studies concluded that although the acquired ion images showed extreme similarities between the two techniques, higher ion intensity and dynamic range can be achieved using DESI.…”

Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 90%

“…22,30,36,41,60,73,104,105,107,125 Lipid distributions in brain tissue sections using both DESI MSI and EASI MSI have been reported in two related studies. 22,30 Both studies concluded that although the acquired ion images showed extreme similarities between the two techniques, higher ion intensity and dynamic range can be achieved using DESI. 22,30 Additional increases in sensitivity in the presence of air flow in AFADESI experiments have enabled rapid whole-body imaging and sensitive metabolite imaging in tissue sections.…”

Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 90%

“…22,30 Both studies concluded that although the acquired ion images showed extreme similarities between the two techniques, higher ion intensity and dynamic range can be achieved using DESI. 22,30 Additional increases in sensitivity in the presence of air flow in AFADESI experiments have enabled rapid whole-body imaging and sensitive metabolite imaging in tissue sections. 40,161 Sensitive lipid imaging with a signal-to-noise ratio of up to 100 in each pixel and a spatial resolution of ~12 µm has been reported using nano-DESI MSI.…”

Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 99%

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Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques

2015

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Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 90%

Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 90%

Section: Localized Analysis and Imaging Applicationsmentioning

confidence: 99%

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Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques

2015

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“…A comparison of the spray spot sizes obtained in DESI and EASI by varying flow rates and solvent compositions was carried out to investigate imaging performance. Although a significant difference in spot size was not found, DESI was comparable with or more sensitive than EASI depending on the targeted analyte . A slow raster speed allows for enhanced extraction of analytes from tissues and minimizes carryovers but the total acquisition time per line increases drastically, while faster raster speeds allow for decreased total imaging time; however, the desorption and ionization efficiency of the analytes can be affected as demonstrated by lower signal intensities.…”

Section: Principles Of Ambient Ms Imagingmentioning

confidence: 92%

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

Pérez

Bagga

Prova

et al. 2018

Rapid Comm Mass Spectrometry

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Ambient mass spectrometry (AMS)-based techniques are performed under ambient conditions in which the ionization and desorption occur in the open environment allowing the direct analysis of molecules with minimal or no sample preparation. A selected group of AMS techniques demonstrate imaging capabilities that can provide information about the localization of molecules on complex sample surfaces such as biological tissues. 2D, 3D, and multimodal imaging have unlocked an array of applications to systematically address complex problems in many areas of research such as drug monitoring, natural products, forensics, and cancer diagnostics. In the present review, we summarize recent advances in the field with respect to the implementation of new ambient ionization techniques and current applications in the last 5 years. In more detail, we mainly focus on imaging applications in topics related to animal whole bodies and tissues, single cells, cancer diagnostics and biomarkers, microbial cultures and co-cultures, plant and natural product metabolomics, and forensic applications. Finally, we discuss new areas of research, future perspectives, and the overall direction that the field may take in the years to come.

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“…DESI-MS method has also proved its potential in many other non-forensic applications such as food control analysis of multiple pesticides on different food surfaces [40], analysis of drugs in wastewater [41], veterinary drugs in feedstuffs [42], lubricant oil additives [43] and clinical diagnostics [21,46]. Research shows great DESI-MS potential in the analysis of lipids, proteins, protein complexes, carbohydrates, and oligonucleotides, specimens from industrial polymers to small organic molecules [44]. Furthermore, the ability to perform spatial in situ imaging of biological samples seems to be particularly useful, especially in case of medical cancer diagnosis [46].…”

Section: Applicationmentioning

confidence: 99%

DESI-MS analysis of human fluids and tissues for forensic applications

Wójtowicz

Wietecha‐Posłuszny

2019

Appl. Phys. A

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Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient ionization technique which application in forensic chemistry appears to be very promising. The technique enables rapid in situ analyses without the need of elaborate sample preparation. Nowadays, many DESI-MS applications in forensics, medical diagnostics and food control have been already reported in the literature. The study summarizes the latest DESI-MS forensic applications in the analysis of biological fluids and tissues, i.e., fingerprints, skin, urine and blood, in which the interest has grown during the recent years. In the publication presented below, the use of blood samples in dried blood spots is also included. The analysis reported covers mainly the determination of medicines and illicit drugs content in biological fluids and tissues, where great DESI-MS potential in screening and drugs identification was recognized. Furthermore, the examples of explosives and condoms components detection are also present. Abbreviations ACN Acetonitrile DBS Dried blood spots DESI-MS Desorption electrospray ionization mass spectrometry DMF N,N-dimethylformamide EtOH Ethanol GC-MS Gas chromatography-mass spectrometry GSR Gunshot residues LC-MS/MS Liquid chromatography tandem mass spectrometry LOD Limit of detection LOQ Limit of quantification MeOH Methanol OTC drugs Over-the-counter drugs PMMA Poly(methyl methacrylate) PTFE Polytetrafluoroethylene RDX Trinitrohexahydro-1,3,5-triazine TFME Thin-film microextraction TLME Thin liquid membrane extraction TLC Thin-layer chromatography TNT 2,4,6-Trinitrotoluene QuEChERS extraction Quick, easy, cheap, effective, rugged, and safe extraction

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Comparisons of ambient spray ionization imaging methods

Cited by 11 publications

References 27 publications

Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques

Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques

Review and perspectives on the applications of mass spectrometry imaging under ambient conditions

DESI-MS analysis of human fluids and tissues for forensic applications

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