Comparisons between needle puncture and chondroitinase ABC to induce intervertebral disc degeneration in rabbits

Yang, Ke; Song, Zhanfeng; Jia, Dingding; Ma, Jianqing; Huo, Yachong; Zhao, Ye; Zhang, Wei; Ding, Wenyuan; Wu, Zhanyong; Yang, Sidong

doi:10.1007/s00586-022-07287-8

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…56,102 In rabbits, formation of disc osteophytes in this chondroitinase ABC model is also much less severe than in the disc puncture model. 101 However, a comparative study of four different rabbit injury models, showed that annular puncture produced the most consistent degeneration. 104 The results from this work emphasize that researchers looking to Considering that the rabbit disc continues to degenerate in an accelerated manner in response to puncture, a different model entirely may be more appropriate for replicating the pathology of early onset human IVDD in which many cell therapies would be clinically applied.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, in this model, facet OA can only be studied in response to mechanical disc injury. Alternative models of IVDD induction include whole motion segment immobilization, 39 whole motion segment overloading, 37,49,50,99,100 intradiscal injection of chondroitinase ABC, 56,101,102 and intradiscal injection of proinflammatory molecules 103 . Overloading has been used in many rodent models 37,50,99 as well as rabbit models, 49,100 allowing for the study of simultaneous disc and facet perturbation.…”

Section: Discussionmentioning

confidence: 99%

“…However, intradiscal injection of chondroitinase ABC, an enzyme that cleaves chondroitin sulfate proteoglycans, is a more popular method of inducing IVDD in large animals due to its ability to initiate the loss of proteoglycans as is seen in the onset of human IVDD 56,102 . In rabbits, formation of disc osteophytes in this chondroitinase ABC model is also much less severe than in the disc puncture model 101 . However, a comparative study of four different rabbit injury models, showed that annular puncture produced the most consistent degeneration 104 …”

Section: Discussionmentioning

confidence: 99%

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Mechanical crosstalk between the intervertebral disc, facet joints, and vertebral endplate following acute disc injury in a rabbit model

Fainor,

Orozco,

Muir

et al. 2023

JOR Spine

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BackgroundVertebral endplate sclerosis and facet osteoarthritis have been documented in animals and humans. However, it is unclear how these adjacent pathologies engage in crosstalk with the intervertebral disc. This study sought to elucidate this crosstalk by assessing each compartment individually in response to acute disc injury.MethodsEleven New Zealand White rabbits underwent annular disc puncture using a 16G or 21G needle. At 4 and 10 weeks, individual compartments of the motion segment were analyzed. Discs underwent T1 relaxation mapping with MRI contrast agent gadodiamide as well T2 mapping. Both discs and facets underwent mechanical testing via vertebra—disc—vertebra tension‐compression creep testing and indentation testing, respectively. Endplate bone density was quantified via μCT. Discs and facets were sectioned and stained for histology scoring.ResultsIntervertebral discs became more degenerative with increasing needle diameter and time post‐puncture. Bone density also increased in endplates adjacent to both 21G and 16G punctured discs leading to reduced gadodiamide transport at 10 weeks. The facet joints, however, did not follow this same trend. Facets adjacent to 16G punctured discs were less degenerative than facets adjacent to 21G punctured discs at 10 weeks. 16G facets were more degenerative at 4 weeks than at 10, suggesting the cartilage had recovered. The formation of severe disc osteophytes in 16G punctured discs between 4 and 10 weeks likely offloaded the facet cartilage, leading to the recovery observed.ConclusionsOverall, this study supports that degeneration spans the whole spinal motion segment following disc injury. Vertebral endplate thickening occurred in response to disc injury, which limited the diffusion of small molecules into the disc. This work also suggests that altered disc mechanics can induce facet degeneration, and that extreme bony remodeling adjacent to the disc may promote facet cartilage recovery through offloading of the articular cartilage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Mechanical crosstalk between the intervertebral disc, facet joints, and vertebral endplate following acute disc injury in a rabbit model

Fainor,

Orozco,

Muir

et al. 2023

JOR Spine

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Injectable hydrogel induces regeneration of naturally degenerate human intervertebral discs in a loaded organ culture model

Cherif,

Li,

Snuggs

et al. 2024

Acta Biomaterialia

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Regenerative effects of platelet-rich plasma releasate injection in rabbit discs degenerated by intradiscal injection of condoliase

Hasegawa,

Akeda,

Yamada

et al. 2023

Arthritis Res Ther

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Background Intradiscal condoliase injection is an alternative therapeutic option for lumbar disc herniation (LDH). However, it is often associated with disc degeneration. Several in vivo studies have demonstrated the regenerative potential of platelet-rich plasma (PRP) in disc degeneration. Thus, we hypothesized that the intradiscal injection of PRP releasate (PRPr), a soluble releasate isolated from PRP, has the potential to regenerate degenerated intervertebral discs (IVDs) induced by condoliase. This study examined the regenerative effects of PRPr on rabbit IVDs degenerated following condoliase injection. Methods Eleven New Zealand white rabbits were used in this study. Condoliase (12.5 mU/10 μl) was injected into two non-contiguous discs (L2-L3 and L4-L5), and L3-L4 disc was left intact as a non-injection control. Saline (20 μl) or PRPr (20 μl) was randomly injected into L2-L3 and L4-L5 discs 4 weeks after the condoliase injection. Disc height (DH) was radiographically monitored biweekly from the day of condoliase injection to 16 weeks post-injection. Changes in DH were expressed as percentage DH (%DH) normalized to the baseline DH. Sixteen weeks after condoliase injection, all rabbits were euthanized, and subjected to MRI and histological analyses. Results Intradiscal injection of condoliase induced a significant decrease in %DH (L2-L3 and L4-L5) to 52.0% at week 4. However, the %DH began to return to normal after saline injection and reached 76.3% at week 16. In the PRPr group, %DH began to recover to normal after the PRPr injection and was restored to 95.5% at week 16. The MRI-modified Pfirrmann grade of the PRPr group was significantly lower than that of the saline group (P < 0.01). Histological analyses showed progressive degenerative changes, including reduction of the NP area and condensation of the matrix in the saline and PRPr groups. The histological score of the PRPr group was significantly lower than that of the saline group (P < 0.01). Conclusions PRPr has great potential to enhance the regeneration of degenerated rabbit IVDs induced by condoliase. The results of this preclinical study suggest that PRPr injection therapy may be indicated for patients with LDH who have poor recovery from disc degeneration after chemonucleolysis treatment with condoliase.

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Comparisons between needle puncture and chondroitinase ABC to induce intervertebral disc degeneration in rabbits

Cited by 4 publications

References 43 publications

Mechanical crosstalk between the intervertebral disc, facet joints, and vertebral endplate following acute disc injury in a rabbit model

Mechanical crosstalk between the intervertebral disc, facet joints, and vertebral endplate following acute disc injury in a rabbit model

Injectable hydrogel induces regeneration of naturally degenerate human intervertebral discs in a loaded organ culture model

Regenerative effects of platelet-rich plasma releasate injection in rabbit discs degenerated by intradiscal injection of condoliase

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