Comparisons among Mg, Zn, Sr, and Si doped nano-hydroxyapatite/chitosan composites for load-bearing bone tissue engineering applications

Ran, Jiabing; Jiang, Pei; Sun, Guanglin; Ma, Zhe; Hu, Jingxiao; Shen, Xinyu; Tong, Hua

doi:10.1039/c6qm00192k

Cited by 56 publications

(30 citation statements)

References 51 publications

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“…Multi-element substituted hydroxyapatites (ms-HAPs) are receiving increasing attention, as in these nanomaterials the typical properties of hydroxyapatite can be modified toward specific biological responses. [1][2][3][4][5][6][7][8][9][10][11][12] In hydroxyapatite, even a small amount of doping can create a significant alteration of critical properties such as solubility, particle size, morphology, specific surface area, porosity, and aspect ratio. [3][4][5][6]9 Moreover, the doping elements will eventually be released in vivo, making these materials as multi-functional systems for the release of biologically active ions.…”

Section: Introductionmentioning

confidence: 99%

<p>Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration</p>

Garbo

Ločs

D’Este

et al. 2020

IJN

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Purpose: Compositional tailoring is gaining more attention in the development of advanced biomimetic nanomaterials. In this study, we aimed to prepare advanced multi-substituted hydroxyapatites (ms-HAPs), which show similarity with the inorganic phase of bones and might have therapeutic potential for bone regeneration. Materials: Novel nano hydroxyapatites substituted simultaneously with divalent cations: Mg 2+ (1.5%), Zn 2+ (0.2%), Sr 2+ (5% and 10%), and Si (0.2%) as orthosilicate (SiO 4 4-) were designed and successfully synthesized for the first time. Methods: The ms-HAPs were obtained via a wet-chemistry precipitation route without the use of surfactants, which is a safe and ecologically friendly method. The composition of synthesized materials was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The materials were characterized by X-ray powder diffraction (XRD), FT-IR and FT-Raman spectroscopy, BET measurements and by imaging techniques using highresolution TEM (HR-TEM), FE-SEM coupled with EDX, and atomic force microscopy (AFM). The ion release was measured in water and in simulated body fluid (SBF). Results: Characterization methods confirmed the presence of the unique phase of pure stoichiometric HAP structure and high compositional purity of all synthesized nanomaterials. The doping elements influenced the crystallite size, the crystallinity, lattice parameters, morphology, particle size and shape, specific surface area, and porosity. Results showed a decrease in both nanoparticle size and crystallinity degree, coupled with an increase in specific surface area of these advanced ms-HAP materials, in comparison with pure stoichiometric HAP. The release of biologically important ions was confirmed in different liquid media, both in static and simulated dynamic conditions. Conclusion: The incorporation of the four substituting elements into the HAP structure is demonstrated. Synthesized nanostructured ms-HAP materials might inherit the in vivo effects of substituting functional elements and properties of hydroxyapatite for bone healing and regeneration. Results revealed a rational tailoring approach for the design of a next generation of bioactive ms-HAPs as promising candidates for bone regeneration.

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Section: Introductionmentioning

confidence: 99%

<p>Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration</p>

Garbo

Ločs

D’Este

et al. 2020

IJN

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“…The healing of fractures requires the use of biocompatible materials with specific physical and chemical properties as well as osteoconductive and bone regeneration capacity [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. In orthopedic surgery, a more difficult situation is associated with bone fragility, like osteoporosis [17,18], where a special attention is required concerning the selection of the graft material.…”

mentioning

confidence: 99%

“…Furthermore, the increasing interest in the development of bio-ceramics based on synthetic hydroxyapatite (HAP) and substituted HAP with various essential elements, such as Mg, Zn, Si and Sr [4][5][6][7][8][9][10][11][12][13][14][15][16] and especially for Sr substituted HAP (HAP-Sr) [11][12][13][22][23][24][25][26][27][28], for bone repair is justified by the growing evidence of Sr beneficial effect on bone regeneration [2,17,20].…”

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confidence: 99%

In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites

Răpuntean¹,

Frangopol²,

Hodisan³

et al. 2019

Rev. Chim.

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The goal of this study was to analyze the response of osteoblasts cultured on strontium substituted hydroxyapatites (HAP-Sr) of well-defined high crystallinity deposited as thin films on glass plates. Up to now, this aspect has not been carefully investigated in the context of bio-ceramics. In this study, we present the osteoblasts activity on synthesized HAP-Sr for different amounts of strontium substitution for calcium within the hydroxyapatite (Ca10(PO4)6(OH)2, HAP) lattice, namely HAP-5%Sr, HAP-10%Sr, HAP-15%Sr and HAP-59.2%Sr (Sr-HAP, of formula Sr10(PO4)6(OH)2), in comparison with stoichiometric pure HAP, chosen as control. Each bio-ceramic was deposited as thin multilayers self-assembled substrate (scaffold) and chemically bonded to the surface of glass plates. These coatings revealed by AFM and SEM imaging a granular texture formed from bio-ceramic nanoparticles. They possessed a high degree of crystallinity, i.e. 68% to 86%, depending on the Sr amount within the HAP lattice, as judged by XRD. Osteoblasts were cultured up to 21days and displayed enhanced adhesion and proliferation particularly evidenced on relatively high strontium contents (especially 5 and 10 weight %, determined by SEM-EDX), where the alkaline phosphatase activity and type I collagen were strongly evidenced. These bio-ceramics showed a high in vitro biocompatibility stimulating the activity of osteoblasts in the process of bone formation. These nano biomaterials can have applications in orthopedic and dental surgery improving the osteointegration as coatings of bone implants as well as for bone repair and regeneration.

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“…There have been many studies demonstrating the importance of trace elements in bone formation (Boanini, Gazzano, & Bigi, ; Ran et al, ). Today, ionic substitutions is one of the most widely used approach to mimic the chemical composition of the bone mineral and to improve the biological performance of calcium phosphate materials (Boanini, Gazzano, & Bigi, ).…”

Section: Introductionmentioning

confidence: 99%

Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone

et al. 2019

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Biomimetic triphasic strontium‐substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO3, (NH2)2COH3PO4, and Sr(NO3)2 as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X‐ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β‐tricalcium phosphate (β‐TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β‐TCP. Sr2+ occupy the Ca(1) site in HA, Ca(3,4,7,8) sites in OCP and Ca(1,2,3,4) sites in β‐TCP. Along with Sr2+ substitution, presence of Mg2+ and Na+ ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture.

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Comparisons among Mg, Zn, Sr, and Si doped nano-hydroxyapatite/chitosan composites for load-bearing bone tissue engineering applications

Abstract: The Si-doped hydroxyapatite/chitosan composite has advantages over Mg, Zn, and Sr doped hydroxyapatite/chitosan composites in terms of bone tissue engineering.

Cited by 56 publications

References 51 publications

<p>Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration</p>

<p>Advanced Mg, Zn, Sr, Si Multi-Substituted Hydroxyapatites for Bone Regeneration</p>

In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites

Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone

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