Comparison the Biodegradation Efficiency of Cypermethrin by Pseudomonas aeruginosa and Enterobactercloacae

Abstract

Two local bacterial isolates were isolated from agriculture soil contaminated with cypermethrin from AlAbaeje village in Baghdad city,Iraq. Primary screening was done to test bacterial ability to growth and resistance to cypermethrin by using nutrient agar plates containing 500 mg L -1 cypermethrin as a sole source of carbon and energy, and incubation at 37 ºϹ for 72 hours. Secondary screening results showed that these two bacterial isolates have the ability to grow and resistance cypermethrin concentration till 3600 mg L -1 in solid MMSM.In lab experiment and under optimal conditions, cypermethrin biodegradation was measured by using FTIR and GC-MS analysis.The results shown that E.cloacae was the best isolate for degrading cypermethrin with percentage 94% while P. aeruginosa with degrading percentage 88%.

Isolation, Characteristics, and Prospects of Using the Ochrobactrum Intermedium Strain in the Degradation of the Cypermethrin Pesticide

“…Many studies examining the toxicity and persistence of cypermethrin indicate the need to develop methods for removing it and its metabolites from the environment. Biodegradation based on the use of microorganisms is the preferred method for breaking down pesticides into simple inorganic chemicals since it is environmentally friendly and cost-effective [33]. Numerous studies have been conducted on isolating, characterizing, and selecting pesticide-degrading microorganisms [34,35].…”

“…Ochrobactrum anthropi has been used to degrade 2,4-dichlorophenoxyacetic acid (2,4-D) [44]; Ochrobactrum anthropi strain L1-W successfully degraded air pollutant Di-2-ethylhexyl phthalate (DEHP) [45]. The effectiveness of bacteria in decomposing pesticides depends on the following environmental factors: pH and temperature [33], initial concentration of pesticide [46,47], optical density of microorganisms, etc. We conducted studies to determine the influence of temperature, initial pesticide concentration, and optical density of the PDB-3 strain.…”