Comparison study of porous, fused-core, and monolithic silica-based C18 HPLC columns for Celestoderm-V Ointment® analysis

Zheng, Jinjian; Patel, Dolly; Tang, Qinglin; Markovich, Robert J.; Rustum, Abu M.

doi:10.1016/j.jpba.2009.06.042

Cited by 34 publications

(19 citation statements)

References 22 publications

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“…Ascentis ® Express column, which is packed with unique 2.7-µm fused-core particles, 23 showed the best performance, i.e., better resolution and peak sharpness with a low back pressure compared to other conventional particulate columns. [24][25][26] Accordingly, almost all constituents of P. koreana extract, including the four triterpenoid saponins, were fairly well resolved within 26 min of elution ( Figure 5). …”

Section: Resultsmentioning

confidence: 94%

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom¹,

Suh²,

Youm³

et al. 2010

Bulletin of the Korean Chemical Society

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Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis ® Express C18 column (4.6 mm × 150 mm, 2.7 µm) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at 30 o C. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 -200 µg/mL for pulsatilloside E, anemoside B4 and cussosaponin C, and 5 -500 µg/mL for pulsatilla saponin H with correlation coefficient (R 2 ) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 -0.2 and 2 -5 µg/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra-and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.

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Section: Resultsmentioning

confidence: 94%

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom¹,

Suh²,

Youm³

et al. 2010

Bulletin of the Korean Chemical Society

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“…Further theoretical and practical aspects [94] and application to formulations are the comparison of porous, fused-core and monolithic C18 columns for the determination of betamethasone valerate and its degradants in an ointment [95] and estradiol in a gel formulation [96]. Monoliths were also used for the assay of triamcinolone, prednisolone and dexamethasone in tablets [97] as well betamethasone [98] in eye drops together with chloramphenicol.…”

Section: Hplc Methodsmentioning

confidence: 99%

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

Gǒrög

2011

Journal of Pharmaceutical and Biomedical Analysis

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“…poly(ethyle) neoxide or polyethylene glycol) (31). Silica based material is the most popular substrate for HPLC columns due to its unique properties such as inertness to a wide variety of analytes, mechanical strength to withstand relatively high pressures, and high efficiency (32). The rods formed, containing both macro and mesopores, are highly porous material (Figure 4).…”

Section: Monolithic Columnsmentioning

confidence: 99%

Fast Analysis of Bioactive Compounds by Reverse Phase Liquid Chromatography

Nogueira

Rostagno

Gomes

et al. 2014

ACS Symposium Series

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The analysis of bioactive compounds in raw bioactive materials by liquid chromatography can prove to be a very difficult task and usually require extended times to adequately separate all components from such complex matrices. Currently there is a great interest in the development of new technology to improve chromatographic performance and reduce the necessary time to achieve the separation between components. Liquid chromatography has seen great improvements in the last decades, especially in stationary phase technology. New stationary phase technologies include sub2 µm particles, partially porous and porous monolithic polymers. These new packing materials are taking the chromatographic performance of separations to a much higher level. Liquid chromatography systems are also seen improvements in term of pressure limits, precision, reproducibility and overall quality of the results. The use of these advanced systems and materials allied to the adoption of carefully planned strategies under optimized conditions can produce excellent chromatographic results with speeds that were thought to be impossible a few years ago. In this context, this chapter discusses current technology available that can be explored to reduce analysis time of bioactive compounds in natural products. It also discusses the influence of most important operational parameters (column In Instrumental Methods for the Analysis and Identification of Bioactive Molecules; Jayprakasha, et al.; ACS Symposium Series; American Chemical Society: Washington, DC, 2014.temperature, mobile phase composition and flow rate/ linear velocity in the chromatographic system and how these variables can be adjusted in order to produce the greatest potential for performing separations.

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Comparison study of porous, fused-core, and monolithic silica-based C18 HPLC columns for Celestoderm-V Ointment® analysis

Cited by 34 publications

References 22 publications

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

Fast Analysis of Bioactive Compounds by Reverse Phase Liquid Chromatography

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