Comparison Study of Oropharyngeal Microbiota in Case of Bronchial Asthma and Chronic Obstructive Pulmonary Disease in Different Severity Levels

Огородова, Л. М.; Федосенко, С. В.; Popenko, Anna; Петров, В. А.; Tyakht, Alexander V.; Saltykova, Irina V.; Deev, I.A.; Куликов, Е. С.; Кириллова, Н. А.; Govorun, Vadim M.; Kostryukova, Elena S.

doi:10.15690/vramn532

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…In addition, N. subflava played an important role in the co-occurrence network, and the reduction of this species may drive the observed shift at the community level. The genus Granulicatella was reported to decrease in COPD patients, 32 which was verified in our study. We also found that Granulicatella sp.…”

Section: Discussionsupporting

confidence: 87%

Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease

Diao

Shen

et al. 2018

COPD

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BackgroundThe role of airway microbiota in COPD is highly debated. Symptomology assessment is vital for the management of clinically stable COPD patients; however, the link between symp toms and the airway microbiome is currently unknown.PurposeThe present study aimed to evaluate the relationship among stable COPD patients.Patients and methodsWe conducted pyrosequencing of bacterial 16S rRNA using induced sputum samples in a Han Chinese cohort that included 40 clinically stable COPD patients and 19 healthy controls.ResultsAlterations in community composition and core bacte rial taxa (Neisseria subflava, etc.) were observed in patients with severe symptoms compared with controls. The co-occurrence network indicated that the key microbiota enriched in COPD patients showed higher expression in patients with severe symptoms. The association pattern of symptoms with the sputum microbiome was obviously different from that of lung function in COPD patients.ConclusionThese findings broaden our insights into the relationship between the sputum microbiota and the symptom severity in COPD patients, emphasizing the role of symptoms in the airway microbiome, independent of lung function.

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Section: Discussionsupporting

confidence: 87%

Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease

Diao

Shen

et al. 2018

COPD

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“…In our study, Psychrobacter , Lactobacillus , Rothia , Prevotella , Neisseria , Streptococcus , Haemophilus , Actinomyces , Leptotrichia , and Aspergillus were observed to have high relative abundances in both the sputum and oropharyngeal samples in individuals with severe AECOPD. According to previous reports, oropharyngeal and sputum samples from COPD patients have high abundances of Haemophilus , Streptococcus , and Prevotella , whereas sputum samples from COPD patients have high abundances of Psychrobacter and Neisseria ( Aguirre et al, 2015 ; Huang and Boushey, 2015 ; Ogorodova et al, 2015 ; Wang et al, 2016 ). In previous reports, Aspergillus has been found to be associated with severe AECOPD, and its presence can lead to decreased lung function in affected patients ( Morris et al, 2008 ; Barberan and Mensa, 2014 ).…”

Section: Discussionmentioning

confidence: 88%

“…The fungal community structure also showed that the differences between individuals were greater than the differences between sampling points. Previous research on microbial communities in oropharyngeal swab and sputum samples has demonstrated high abundances of Haemophilus , Prevotella , and Streptococcus ( Huang and Boushey, 2015 ; Ogorodova et al, 2015 ). These common genera were also detected in our study, with high linear correlations in the paired samples.…”

Section: Discussionmentioning

confidence: 99%

Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease

et al. 2017

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Growing evidence suggests that the airway microbiota might be involved in acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Understanding this relationship requires examination of a large-scale population for a long duration to accurately monitor changes in the microbiome. This type of longitudinal study requires an appropriate sampling strategy; two options are the collection of sputum or oropharyngeal swabs. Comparative analysis of the changes that occur in these two specimen types has not been previously performed. This observational study was conducted to explore oropharyngeal microbial community dynamics over time and to examine the relationship between oropharyngeal swabs and sputum. A total of 114 samples were collected from four patients suffering from severe AECOPD. Bacterial and fungal communities were evaluated using 16S rRNA and ITS sequencing. Inter-individual differences were found in bacterial community structure, but the core genera were shared by both sample types and included 32 lineages. Most of the core genera were members of the phyla Proteobacteria, Firmicutes and Ascomycota. Although the oropharyngeal samples showed higher bacterial alpha diversity, the two sample types generated rather similar taxonomic profiles. These results suggest that the sputum microbiome is remarkably similar to the oropharyngeal microbiome. Thus, oropharyngeal swabs can potentially be used instead of sputum samples for patients with exacerbation of COPD.

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“…These studies predominantly focused on bacterial communities of lower airway using induced sputum or bronchoalveolar lavage fluid (BALF) samples 7–9. However, microbiome studies of throat samples are limited 10,11. Moreover, the goal of these studies was to identify differences of throat flora between COPD and asthma patients,10,11 restricting deep analyses for the throat microbiome of COPD.…”

Section: Introductionmentioning

confidence: 99%

“…However, microbiome studies of throat samples are limited 10,11. Moreover, the goal of these studies was to identify differences of throat flora between COPD and asthma patients,10,11 restricting deep analyses for the throat microbiome of COPD.…”

Section: Introductionmentioning

confidence: 99%

Characterization of throat microbial flora in smokers with or without COPD

Diao¹,

Shen²,

Du³

et al. 2017

COPD

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The study aimed to determine the relationship between throat microbiome and COPD. Sixty-five Chinese males (n=20, smokers without COPD; n=45 smokers with COPD) were included. Nonmetric multidimensional scaling indicated differences of microbiome between COPD and controls, but no difference was observed between COPD patients with differing degrees of lung function or disease severity. Rarefaction analyses suggested that operational taxonomic units (OTUs, species-level) richness decreased in COPD. The dominant taxa between COPD and controls were similar, but the proportions of taxonomic distribution were different. The dominant phyla were Bacteroidetes, Proteobacteria, Firmicutes and Fusobacteria. The dominant genera were Haemophilus, Leptotrichia, Porphyromonas, Fusobacterium, Veillonella, Streptococcus, Neisseria and Prevotella. Two dominant OTUs, otu3 (Veillonella_dispar) and otu4 (Streptococcus_unclassified), were identified. Otu3 and its father-level taxa, which were negatively correlated with predicted percent of forced expiratory volume in a second (FEV1%pred), were increased in COPD. By contrast, otu4 and its father-level taxa, which were positively correlated with FEV1%pred, were decreased in COPD. Otu4 also showed a slight potential as a COPD biomarker. To conclude, the throat microbiome was different between smokers with or without COPD, which is similar to findings from the lower respiratory tract. This study may strengthen our understanding of the relationship between microbiomes of different airway sites and COPD.

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Comparison Study of Oropharyngeal Microbiota in Case of Bronchial Asthma and Chronic Obstructive Pulmonary Disease in Different Severity Levels

Cited by 8 publications

References 36 publications

Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease

Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease

Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease

Characterization of throat microbial flora in smokers with or without COPD

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