Comparison Studies of Whole Blood Stored in ACD and CPD and with Adenine

Abstract

Using 70% 24‐hour posttransfusion survival as one of the criteria of preservation, ACD and CPD anticoagulant solutions with and without adenine were tested after storage for 28, 35, or 42 days. At 28 days, all solutions had average survivals of 70% or better. The average survival values for the ACD and CPD solution groups were less than 70% at 35 and 42 days. However, both solutions with adenine had a group average value over 70% even after 35 or 42 days of storage. The average survival values between the two anticoagulants alone, were not significantly different at each time period. The two anticoagulants when adenine was included had increased survival and there was no statistical difference between the levels. In comparing two units obtained from the same subject, survival percentages were significantly higher in almost every recipient when the adenine‐supplemented stored blood was used. Other chemical determinations did not show significant alterations and no toxic effects were observed in the recipients. Since all units containing adenine had survival values greater than 70% in the 28‐ and 35‐day periods, these units would appear to have been effectively preserved and blood stored under these conditions could be used in routine transfusions, reserving units stored 42 days for emergency use.

24‐Hour Survival of ACD‐ and CPD‐Stored Red Cells

“…In our study, however, there was no significant difference in 24-hour posttransfusion survival between red cells collected in ACD and those collected in CPD anticoagulant. Our data are in accord with those reported by SHIELDS [20] and by ORLINA and JOSEPHSON 1171. The differences in the results reported by various investigators may be explained either by the number of cases studied or by the fact that we utilized an ADA procedure to measure the posttransfusion survival of red cells, whereas other investigators have used a radioactive 51Cr-labelling procedure.…”

Effect of Plasma Removal on Blood Stored in ACD with Adenine

“…deterioration that can be safely tolerated tends to occur by 21 days. In contrast, addition of small amounts of adenine appears to slow the process, permitting an extension of storage to 35 days or more.4~ 6, [8][9][10] Whole blood collection solutions, with or without additives such as adenine, are diluted by the blood plasma. Prior studiess.…”

Hemoglobin function in stored blood

“…First, it seemed desirable to study the hemoglobin function of blood stored in ACD supplemented with adenine since this additive has received considerable recent attention from military (2) and civilian centers (3,4). Accordingly, we have obtained serial oxygen dissociation curves on paired units of ACD blood, stored with and without adenine.…”