Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance

Cobey, Susan

doi:10.1051/apido:2007029

Cited by 115 publications

(112 citation statements)

References 66 publications

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“…Although Cobey (2007) found that the colony environment is indispensable for the start of oogenesis in the ovaries, the results herein showed that even in queens maintained outside of the colony environment, follicle differentiation can advance until separation of the oocyte and pre-nurse cells, which is just one step short of the ovarian development seen in queens maintained inside a colony. Therefore, the colonial environment can facilitate ovarian development, and its absence may delay ovarian development and lead to more extensive structural disorganization of the ovarioles and cell death.…”

Section: Discussioncontrasting

confidence: 59%

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Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

2015

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-Newly emerged virgin queens are frequently imprisoned in cages either inside or outside of colonies before delivery to a new hive. Ovary integrity and proper functioning is the primary factor in a queen's success as the colony mother. In this work, histological studies on ovaries are used to evaluate the effect of virgin queen imprisonment both out and inside the colony. The results show that the ovarian follicles of virgin queens maintained out of the colony advance only until the beginning of differentiation of oocytes and nurse cells, the vitellarium does not differentiate, and cell death is observed. For virgin queens caged inside a colony, oogenesis progresses until nurse cell and oocyte differentiation is completed, and the vitellarium shows initial differentiation. The results suggest that the best method for a head start of a queen's fertility is to maintain her inside the colony until introduction into a new hive.apiculture / caged queen / cell death / egg production / histology

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Section: Discussioncontrasting

confidence: 59%

“…Although studies suggest that this technique facilitates apicultural work, management flexibility and efficiency in producing queens, it does not generate the same results as those achieved with queens that mate freely (Cobey 2007).…”

Section: Introductionmentioning

confidence: 90%

Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

2015

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“…SDI queens and naturally mated queens have the potential to differ along many dimensions beyond the contents of their spermathecas, including differences in the early handling of SDI queens related to insemination (Cobey 2007). Given that caged SDI queens had a different effect on worker ecdysteroid titers than did caged naturally mated queens, we suggest that chemical signals differentially produced by SDI queens may be responsible for the effects revealed in the controlled cage environment.…”

Section: Ecdysteroid Titers Were Unrelated To Ovary Activation and Ovmentioning

confidence: 86%

Queen mandibular pheromone modulates hemolymph ecdysteroid titers in adult Apis mellifera workers

Trawinski

Fahrbach

2018

Apidologie

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-To test the hypothesis that exposure to queen mandibular pheromone (QMP) modulates ecdysteroid production in adult worker honey bees, ecdysteroids were measured in hemolymph and other tissues of individual adult worker honey bees reared with or without QMP in cages and field colonies. Ecdysteroid titers were higher in caged workers exposed to QMP continuously from the first day of adult life than in workers reared without QMP. Statistical cluster analysis suggested the possibility that a subgroup of workers (Bresponders^) is more sensitive to QMP in this regard than other workers. In 12-day-old workers, ecdysteroid titers in workers reared in queenright (QR) colonies were similar to those observed in cages with QMP, but lower than those in queenless (QL) colonies. Differences in number of ovarioles or degree of ovarian activation did not correlate with hemolymph ecdysteroids. Previous studies have demonstrated ecdysteroids in hemolymph in very young adult workers and in workers in QL colonies; the present study indicates that production of ecdysteroids occurs in older adult worker honey bees in the absence of morphological signs of ovarian activation, with cage studies revealing a modulatory role for QMP masked in the complex environment of the hive.ecdysteroids / ovary / queen mandibular pheromone / queenless / queenright

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“…For this reason, in mass production honey bee queens are usually inseminated with small doses of semen. Naturally mated queens store in their spermatheca 4 to 7 million sperm cells (5). Therefore, well inseminated instrumentally queens should have also at least 4 million spermatozoa.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Number of spermatozoa in the spermatheca of honey bee queens inseminated with small doses of semen and kept in an incubator in cages with different numbers of workers

Gąbka¹,

Muszyńska²,

Zajdel³

2016

Medycyna Weterynaryjna

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Collection of honey bee drone semen is time consuming. For this reason, in mass production honey bee queens are usually inseminated with small doses of semen. Naturally mated queens store in their spermatheca 4 to 7 million sperm cells (5). Therefore, well inseminated instrumentally queens should have also at least 4 million spermatozoa. However Wilde (11) did not observe any differences in the strength of colonies during the entire season and honey production on the brood area and productivity in the colonies with instrumentally inseminated queens with a dose of 8, 4 and only 2 µl of semen. The higher the dose of semen, the higher the number of spermatozoa in the spermatheca but the dose of 8 µl is sufficient (14, 15). However, Bieńkowska et al.(1) received significantly more spermatozoa in queens inseminated with 3, 4 and 6 µl of semen than with 8 µl. Double insemination is more effective. Queens inseminated once have less sperms than those inseminated twice with the same divided dose of semen (1,9,14,15). Gąbka (6) stated that queens inseminated once with 10 µl of semen have similar number of sperms in their spermatheca as those inseminated with 2 × 2 µl but significantly less than those inseminated with 2 × 4 µl of semen. Two inseminations with each dose of 6 µl give even better results than one insemination with 20 µl (15). The access of attendant bees significantly affects the number of spermatozoa in the spermatheca (12,13,16,18). It is best if queens are kept with at least 350 workers after insemination (16,17). The access of workers also affects the clearing of excess semen from oviducts (16). According to Gontarz et al. (8) queens should be keep with attendant bees even before insemination because the period prior to insemination affects condition of their oviducts after insemination. However Wilde (10) did not observe any differences on the brood area and productivity in the colonies with instrumentally inseminated queens kept before and after insemination in different conditions.The aim of this study was to investigate the number of spermatozoa entering the spermatheca of queens Gąbka J., Muszyńska R., Zajdel B.Number of spermatozoa in the spermatheca of honey bee queens inseminated with small doses of semen and kept in an incubator in cages with different numbers of workers SummaryIn mass production, instrumentally inseminated honey bee queens are usually kept in mailing cages with a low number of workers and inseminated with small doses of semen. Inseminated queens should have more than 4 mln sperms in their spermatheca. The aim of this study was to investigate the number of spermatozoa entering the spermatheca of queens inseminated with different small doses of semen. Ninety queens were divided into six groups and inseminated as follows: 1 µl, 2 µl or 3 µl of semen at the age of 7 days and 2 × 1 µl, 2 × 2 µl or 2 × 3 µl at the age of 7 and 9 days. Queens were kept in the mailing cages with 15 or 25 attendant bees before and after instrumental insemination. No significant differences were found i...

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Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance

Cited by 115 publications

References 66 publications

Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

Queen mandibular pheromone modulates hemolymph ecdysteroid titers in adult Apis mellifera workers

Number of spermatozoa in the spermatheca of honey bee queens inseminated with small doses of semen and kept in an incubator in cages with different numbers of workers

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