Comparison Results of Analytical Profile Index and Disc Diffusion Antimicrobial Susceptibility Test to Technical Dedicated Reasonable 300b Method

Abstract

Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibuktikan dengan pemeriksaan kulturdarah yang dilanjutkan dengan uji kepekaan antibiotika. Metode pemeriksaan dapat dilakukan secara manual atau automatis baiksemiautomatis ataupun automatis penuh. Metode manual relatif tidak memerlukan biaya yang besar dibandingkan metode automatisasi.Penelitian ini merupakan analisis observasional dengan desain potong lintang. Metode identifikasi manual memakai metode API danuji kepekaan antibiotika metode difusi cakram antibiotika Kirby Bauer. Kedua metode ini dibandingkan dengan metode semiautomatisTDR-300B. Metode automatis penuh VITEK 2 digunakan sebagai metode rujukan untuk menilai kinerja metode konvensional dansemiautomatis. Bakteri penyebab infeksi aliran darah didominasi Gram negatif kebanyakan Eschericia coli dan Klebsiella pneumonia.Ketepatan metode identifikasi API terhadap VITEK 2 sebesar 87,87%, ketepatan identifikasi metode TDR-300B terhadap metode VITEK2 adalah 90,9%. Hasil ketepatan uji kepekaan antibiotika metode konvensional difusi cakram antibiotika Kirby Bauer terhadap metodeVITEK 2 adalah 84,64%. Ketepatan uji kepekaan antibiotika metode TDR-300B terhadap metode VITEK 2 sebesar 82,5%. Ketepatanmetode API terhadap metode TDR-300B sebesar 84,84%. Ketepatan uji kepekaan antibiotika metode konvensional terhadap metodeTDR-300B sebesar 78,21%. Hasil metode identifikasi dan uji kepekaan antibiotika konvensional tidak berbeda bermakna secara statistikdengan metode semiautomatis TDR-300B. Metode identifikasi dan uji kepekaan antibiotika konvensional masih dapat dipercaya terutamauntuk daerah dengan keterbatasan biaya atau pemeriksaan masih sedikit.

Automation for the identification of Pseudomonas aeruginosa: Comparison of TDR-300B, VITEK®2 and VITEK®-MS

“…The positive predictive value (PPV) and accuracy, however, were lower in TDR-300B than VITEK ® 2 ( Table 4). The Fisher' exact value for both comparison was >0.05, so there was no significant differences in the capability of and Gram-positive bacteria species [11]. In the present study, comparison of TDR-300B and VITEK ® 2 to VITEK ® -MS as gold standard showed that VITEK ® 2 has higher accuracy (92.31%) and PPV (92.00%) than TDR-300B (80.77% and 84.00%).…”

“…Many investigations have been conducted on VITEK ® 2 and, they showed the system was to give reliable results [6][7][8][9]. On the other hand, despite having similar system that is colorimetry based on the biochemical test array TDR-300B is relatively new and its performance has not been well reported [10]; one study thus far was reported by Sugiartha et al, 2017 [11]. In the developing country like Indonesia, this colorimetry-based identification system seems appropriate.…”

A Mini-Review on Commonly used Biochemical Tests for Identification of Bacteria

“…After incubation the change in the color of all wells in noted. All the results are composed and a profile number is obtained which is then compared with profile numbers available online or in a commercial codebook for identification of bacterial species (Sugiartha et al 2018).…”