Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction

Kang, Qiaozhen; Chen, Sisi; Li, Shufang; Wang, Bo; Liu, Xin; Hao, Limin; Lu, Jike

doi:10.1016/j.ijbiomac.2018.11.215

Cited by 99 publications

(44 citation statements)

References 35 publications

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“…Fungal polysaccharides are an important class of functional biomacromolecules that exist in edible and medicinal fungi [7]. Ganoderma, one of the most popular fungi species in China and other Asian countries, has been shown to promote health and longevity [8]. Polysaccharides are the primary bioactive components in Ganoderma species, and they have been demonstrated to possess various biological activities, such as immunomodulatory [9], anti-aging [10], anti-tumor [11], and antioxidant activities [8].…”

Section: Introductionmentioning

confidence: 99%

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Guan

et al. 2021

Antioxidants

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Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma leucocontextum fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 and 14.07 kDa, respectively. Additionally, GLP-1 and GLP-2 had a similar chain conformation, crystal structure, and molecular surface morphology. Moreover, GLP-1 exhibited stronger antioxidant activities than GLP-2 in five different assays: 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical, superoxide anion radical, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). The main linkage types of GLP-1 were found to be →4)-α-D-Glcp-(1→, →4)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, →6)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, and Glcp-(1→ by methylation analysis and nuclear magnetic resonance (NMR) spectroscopy. In addition, GLP-1 could protect NIH3T3 cells against tert-butyl hydroperoxide (tBHP)-induced oxidative damage by increasing catalase (CAT) and glutathione peroxidase (GSH-Px) activities, elevating the glutathione/oxidized glutathione (GSH/GSSG) ratio, and decreasing the malondialdehyde (MDA) level. These findings indicated that GLP-1 could be explored as a potential antioxidant agent for application in functional foods.

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Section: Introductionmentioning

confidence: 99%

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Guan

et al. 2021

Antioxidants

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“…These results indicated that ultrasonic and cellulase treatment during the extraction process induced changes in the Mw distributions. The same phenomenon was also observed in previous reports (Kang et al, ; Li & Wang, ).…”

Section: Resultsmentioning

confidence: 99%

Compared extraction methods on the physicochemical properties, antioxidant activity, and optimization of enzyme‐assisted extraction of polysaccharides from Gynura medica

Li-gen

2019

J Food Process Preserv

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The effects of hot water extraction (HWE), enzyme‐assisted extraction (EAE), ultrasonic‐assisted extraction (UAE), and ultrasonic‐enzyme‐assisted extraction (UEAE) methods on the extraction yield, physicochemical properties, and antioxidant activities of Gynura medica polysaccharides (GMPs) were investigated. The GMPs extracted by four methods were designated GMPs‐H, GMPs‐E, GMPs‐U, and GMPs‐UE, respectively. Meanwhile, the EAE method was further optimized by response surface methodology (RSM). The results showed that EAE and UEAE methods both markedly enhanced the yield of GMPs. Four kinds of GMPs possessed different physicochemical properties including molecular weight distributions and surface morphology. EAE had higher scavenging abilities for DPPH radical compared to other methods. The optimal conditions for GMPs were predicted to be cellulase amount of 155 (mg/g), extraction time of 48.4 min and pH of 7.9, with a predicted yield of 11.98%. Under the optimal conditions, the yield of polysaccharides was 12.03%, which matched the predicted value well. Practical applications Enzyme‐assisted extraction (EAE) is an environmentally friendly method that can be used to improve the extraction of active ingredients from various natural sources. Our results demonstrated that EAE could enhance the extraction yield of polysaccharides which was the most important active ingredient. In addition, Gynura medica polysaccharides possess good antioxidant activity, which means that Gynura medica polysaccharides may be a novel source of natural antioxidant in functional food.

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“…A large number of reports have indicated that GLP is an active substance with functions of immunoregulation, antioxidation, antiaging, and antitumor [40][41][42]. However, the role of GLP in MODS model mice needs further research.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Hepatoprotections of Ganoderma lucidum Polysaccharides against Multiple Organ Dysfunction Syndrome in Mice

Zhang

Yuan

Wang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Background. The liver is one of the most commonly affected organs in multiple organ dysfunction syndrome (MODS). In recent years, there have been many studies on Ganoderma lucidum polysaccharides (GLP), but the role of GLP in MODS is still unclear. The purpose of this work was to explore the antioxidant, anti-inflammatory, and protective effects of GLP on the liver in MODS model mice. Methods. The characteristic properties of GLP were processed by physicochemical analysis. The MODS models were successfully established with intraperitoneal injection of zymosan in Kunming strain mice. The antioxidant, anti-inflammatory, and hepatoprotective effects of GLP were processed both in vitro and in vivo by evaluating the oxidative parameters, inflammatory factors, and liver pathological observations. Results. The characterization analysis revealed that GLP was a pyranose mainly composed of glucose with the molecular weights (Mw) of 8309 Da. The experimental results proved that GLP had potential hepatoprotection possibly by improving the antioxidant status (scavenging excessive oxygen radicals, increasing the antioxidant enzyme activities, and reducing the lipid peroxide), alleviating the inflammatory response (reducing the inflammatory factor levels), and guaranteeing the liver functions. Conclusions. This research suggested that GLP had the potential to be developed as a natural medicine for the treatment of multiple organ failure.

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Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction

Cited by 99 publications

References 35 publications

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Compared extraction methods on the physicochemical properties, antioxidant activity, and optimization of enzyme‐assisted extraction of polysaccharides from Gynura medica

Characterization and Hepatoprotections of Ganoderma lucidum Polysaccharides against Multiple Organ Dysfunction Syndrome in Mice

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