Comparison of δ¹⁸O and δ¹³C values between tree-ring whole wood and cellulose in five species growing under two different site conditions

Abstract

Wood material from the sapwood of the studied tree species is as useful as cellulose for studying environmental effects on tree-ring δ(18)O and δ(13)C values at a short-term scale as considered in most ecophysiological studies. The more variable response of oak may require further investigations.

Mixture reduces climate sensitivity of Douglas-fir stem growth

“…This is in line with the results of Weigt et al (2015), who also examined the resistance of European beech and Douglas-fir. But the species-specific differences must be reflected in the context of generally higher absolute increment rates of Douglas-fir, especially in mixed stands.…”

Contamination risk of stable isotope samples during milling

“…This offset was most prominent and consistent in samples that were milled with more material (40 mg), where they deviated on average by −1.053 ‰ (Table ). These values fall within the range of previously published work on wood‐cellulose comparisons of conifers . Interestingly, the offset became reduced in the samples milled with less material (10 mg) and even reversed in the treatment with the highest abrasion: the 10‐mg–3‐ball–10‐min category showed cellulose being even more lower δ 13 C values than whole wood (Fig.…”

“…These values fall within the range of previously published work on wood-cellulose comparisons of conifers. [48,49] Interestingly, the offset became reduced in the samples milled with less material (10 mg) and even reversed in the treatment with the highest abrasion: the 10-mg-3-ball-10-min category showed cellulose being even more lower δ 13 C values than whole wood ( Fig. 3(c)).…”

Oxygen isotope fractionations across individual leaf carbohydrates in grass and tree species

“…Leaf α ‐cellulose extraction and purification were performed in an ultrasonic bath and specifically described in a recent study (Weigt et al ): In brief, small pieces of plant material (~100 mg) were packed in Teflon filter bags (Ankom Technology, Macedon, NY, USA) and transferred to sodium chlorite solutions (pH 4–5) to remove lignin at 70 °C for 48 h (Fisher Scientific, Reinach, Schweiz). After a washing step with deionized water for 2 h, samples were placed in a sodium hydroxide solution without heating for 45 min to remove fats, oils, tannins and hemicellulose.…”