Comparison of XAD‐2 Column and Blue Cotton Batch Techniques for Isolation of Metabolites of 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline

Abstract

In studies on the metabolism of 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) in the rat, two methods were used to concentrate IQ and its metabolites in urine: XAD‐2 columns and blue‐cotton extraction. These methods were compared as to the total recovery of *14C‐label and the results of high‐performance liquid chromatography (HPLC) of the main metabolites. In the HPLC analysis, three major peaks in the polar region and two adjacent peaks in the nonpolar region having radioactivity were found in the urine from rats given *14C‐IQ. XAD‐2 columns adsorbed approximately 65% and blue cotton 23% of the applied isotope from the urinary metabolites. Both methods efficiently adsorbed and totally released the relatively nonpolar compounds in a mixture of metabolites, including the compound administered, IQ. However, they failed to retain the more polar metabolites. Thus, both the XAD‐2 column and blue cotton adsorption techniques may be useful mainly to concentrate nonpolar compounds from larger volumes of aqueous solutions.

Blue cotton, Blue Rayon and Blue Chitin in the analysis of heterocyclic aromatic amines—a review

“…Using Blue Cotton, IQ, MeIQx, DiMeIQx, PhIP have often been detected, but also some HCAs that are not commonly found in fried foods have been identified, for example 4-OH-PhIP in broiled beef [30], 4-CH 2 OH-8-MeIQx in beef extract [31] and 7,9-DiMeIgQx in beef extract [28]. Blue Cotton has been used for HA extraction from both human urine [32][33][34], and rat urine [35,36]. In addition, but not included in the table, there are also some reports on the use of Blue Cotton for the extraction of HCAs from dialysis fluids, human plasma and human cataract lenses [37][38][39].…”

Development and evaluation of “thermoelectric power-generating thread” using carbon nanotube-coated threads

“…21) Cotton dyed with C.I.Reactive Blue 21 is called blue cotton because it becomes blue, and is used to concentrate various organic substances. [22][23][24][25][26][27] C.I.Reactive Blue 21 also functions as a dispersant for CNT as described above. Therefore, we thought that it could be expected to capture CNT.…”