Comparison of Water Relaxation Time in Serum Albumin Solution Using Nuclear Magnetic Resonance and Time Domain Reflectometry

Fukuzaki, Minoru; Miura, Norihiko; Shinyashiki, Naoki; Kurita, Daisuke; Shioya, Sumie; Haida, Munetaka; Mashimo, Satoru

doi:10.1021/j100001a064

Cited by 53 publications

(55 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, the dielectric relaxation time (τ D ) indicates a bimodal behavior with one component of 10 ps which is similar to ordinary water and another of 10 ns which is thousand times slower compared to bulk water. [11][12][13][14] Secondly, comparing the position of the steady state absorption or emission maxima of many probe molecules in the water pools with those in different solvents [26][27][28] (or comparing present data on TNS with ref. 19) the static dielectric constant (ε 0 ) of the water pool of the microemulsions seems to be close to that of alcohol (i.e.…”

Section: Time Resolved Studiesmentioning

confidence: 99%

“…Recently several groups used the time dependent Stokes shift 5-10 , pulsed NMR and dielectric relaxation techniques [11][12][13][14] to unravel the dynamics of the water molecules in various organized media such as cyclodextrins 5,6 , reverse micelle 4,7-9 and micelles. 10 The results of the dynamic Stokes shift studies indicate that while ordinary water molecules relax in the subpicosecond time scale, inside these organized assemblies the solvation dynamics becomes several thousand times slower and occurs in the nanosecond timescale.…”

mentioning

confidence: 99%

“…[1][2][3] The behavior of water molecules present at such interfaces and perturbed by local electrostatic and hydrogen bond interactions are of special interest because of the unique role played by the water molecules in many biological processes. Recently several groups used the time dependent Stokes shift [5][6][7][8][9][10] , pulsed NMR and dielectric relaxation techniques [11][12][13][14] to unravel the dynamics of the water molecules in various organized media such as cyclodextrins 5,6 , reverse micelle 4,7-9 and micelles. 10 The results of the dynamic Stokes shift studies indicate that while ordinary water molecules relax in the subpicosecond time scale, inside these organized assemblies the solvation dynamics becomes several thousand times slower and occurs in the nanosecond timescale.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Intramolecular Charge Transfer near a Hydrophobic Surface. 2,6-p-Toluidinonaphthalene Sulfonate in a Reverse Micelle

et al. 1998

View full text Add to dashboard Cite

The behavior of an organic molecule at an interface between two bulk media is often significantly different from that in either of the bulk medium. Since the polarity and viscosity at the interface are often very much different from those of the bulk media, the structure, dynamics and reactivity of an organic or biomolecule at an interface differ markedly from those in the bulk. Interestingly most natural and biological processes occur at such interfaces or confined systems, such as proteins, biomembranes and vesicles. As a result in recent years several new experimental and theoretical techniques have been used to study such systems. Several groups have used surface second harmonic and surface sum frequency generation and picosecond total internal reflection spectroscopy to study the structure and the dynamics at various interfaces.1-3 The behavior of water molecules present at such interfaces and perturbed by local electrostatic and hydrogen bond interactions are of special interest because of the unique role played by the water molecules in many biological processes. Recently several groups used the time dependent Stokes shift 5-10 , pulsed NMR and dielectric relaxation techniques [11][12][13][14] to unravel the dynamics of the water molecules in various organized media such as cyclodextrins 5,6 , reverse micelle 4,7-9 and micelles. 10 The results of the dynamic Stokes shift studies indicate that while ordinary water molecules relax in the subpicosecond time scale, inside these organized assemblies the solvation dynamics becomes several thousand times slower and occurs in the nanosecond timescale. [5][6][7][8][9][10] In hydrocarbon solvents at concentrations above ≈1 mM certain surfactants (e.g. sodium dioctyl sulfosuccinate, AOT) form so called reverse micelles with average aggregation number 20 and radius 15 Å, in which the polar headgroups point inwards. 4,7-9,21-28 On addition of water to a solution of AOT in a hydrocarbon solvent a microemulsion is formed which consists of nanometer sized water droplets ("water pool") surrounded by a layer of the surfactant molecule. In heptane the radius (r w ) of such a water pool is about 2w 0 (in Å) where w 0 is the number ratio of the water to the surfactant molecules.4,25 Such microemulsions are simple yet interesting models of biological membranes and the water molecules confined in biological systems. In such a water pool, the water molecules at the peripheries of the pool are strongly held by the polar or ionic headgroups of the surfactants and are thus "bound", while those at the center of the pool are relatively "free". The amount of free and bound water molecules present in such a microemulsion has been estimated and tabulated by many workers. 4,22,23 Recently Bright et al. 7 and our group 8,9 separately reported that in such a water pool of a microemulsion the solvent relaxation times are about 8 ns when the pool is small (r w <10 Å) and 2 ns for bigger water pools. Such nanosecond solvation times are several thousand times slower than the subpicosecond rela...

show abstract

Section: Time Resolved Studiesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Intramolecular Charge Transfer near a Hydrophobic Surface. 2,6-p-Toluidinonaphthalene Sulfonate in a Reverse Micelle

et al. 1998

View full text Add to dashboard Cite

show abstract

“…In general, for isotropic motion, the NMR correlation time is more closely related to the dielectric-relaxation time because the latter is three times the former (e.g., Carrington and McLachlan, 1967). Furthermore, the relation is applicable to the correlation time and the relaxation time for bound H:O in biopolymer solutions (Fukuzaki et al, 1992(Fukuzaki et al, , 1995.…”

Section: Introductionmentioning

confidence: 99%

Dielectric-Relaxation Spectroscopy of Kaolinite, Montmorillonite, Allophane, and Imogolite under Moist Conditions

Ishida

Makino

Wang

2000

Clays and clay miner.

100

View full text Add to dashboard Cite

Abstract--The dielectric behavior of kaolinite, montmorillonite, allophane, and imogolite samples adjusted to a water potential of 33 kPa was examined using a time-domain reflectometry method over a wide frequency range of 103-10 ~~ Hz. A dielectric relaxation peak owing to bound H20 was observed. The observation of this peak required the precise determination of the contributions of dc conductivity. The peak is located at 10 MHz, indicating that the relaxation time of the bound H20 is approximately ten times longer than the relaxation time of bound H20 with organic polymers, such as an aqueous globular-protein solution. The structure of bound H20 differs between phyllosilicates and amorphous phases, based on differences in relaxation strength and the pattern of distribution of the relaxation times. The dielectric process involving rotation of bulk H20 molecules was also observed at 20 GHz. The relaxation strength of bulk H20 increased with an increase in the water content. The interfacial polarization in the diffuse double layer occurred only in montmorillonite and kaolinite, indicating that mechanisms involving the Maxwell-Wagner and surface-polarization effects cannot be extended to include allophane and imogolite. Although these results suggest that additional work is required, a tentative conclusion is that a tangential migration of counter-ions along clay surfaces may be important.

show abstract

“…While little information, if any, is available about UHF dielectric properties of the cell nucleus, several Takashima et al [1984] (DNA 10 mg/ml), Foster et al [1984] (DNA 24, 48, and 70 mg/ml), and Fukuzaki et al [1995] (Albumin).…”

Section: Cell Nucleus Dielectric Propertiesmentioning

confidence: 99%

Theoretical evaluation of dielectric absorption of microwave energy at the scale of nucleic acids

Vanderstraeten

Vorst

2004

Bioelectromagnetics

View full text Add to dashboard Cite

A theoretical model is proposed for the evaluation of dielectric properties of the cell nucleus between 0.3 and 3 GHz, as a function of its nucleic acids (NA) concentration (CNA). It is based on literature data on dielectric properties of DNA solutions and nucleoplasm. In skeletal muscle cells, the specific absorption rate (SAR) ratio between nucleoplasm and cytoplasm is found to be larger than one for CNA above 30 mg/ml. A nearly linear relationship is found between CNA and this nucleocytoplasmic SAR ratio. Considering the nanoscale of the layer of condensed counterions and bound water molecules at the NA-solution interface, the power absorption per unit volume is evaluated at this precise location. It is found to be between one and two orders of magnitude above that in muscle tissue as a whole. Under realistic microwave (MW) exposure conditions, however, these SAR inhomogeneities do not generate any significant thermal gradient at the scale considered here. Nevertheless, the question arises of a possible biological relevance of nonnegligible and preferential heat production at the location of the cell nucleus and of the NA molecules.

show abstract

Comparison of Water Relaxation Time in Serum Albumin Solution Using Nuclear Magnetic Resonance and Time Domain Reflectometry

Cited by 53 publications

References 5 publications

Intramolecular Charge Transfer near a Hydrophobic Surface. 2,6-p-Toluidinonaphthalene Sulfonate in a Reverse Micelle

Intramolecular Charge Transfer near a Hydrophobic Surface. 2,6-p-Toluidinonaphthalene Sulfonate in a Reverse Micelle

Dielectric-Relaxation Spectroscopy of Kaolinite, Montmorillonite, Allophane, and Imogolite under Moist Conditions

Theoretical evaluation of dielectric absorption of microwave energy at the scale of nucleic acids

Contact Info

Product

Resources

About